本文選題：食管癌　切入點：外泌體　出處：《解放軍醫(yī)學(xué)雜志》2017年04期

【摘要】：目的探討食管癌KYSE410細(xì)胞來源的外泌體對腫瘤細(xì)胞遷移及侵襲能力的影響及其機制。方法超速離心法分離食管癌KYSE410細(xì)胞培養(yǎng)上清中的外泌體;采用透射電鏡及Western blotting觀察所獲得的外泌體的形態(tài)及其標(biāo)志物;共聚焦顯微鏡觀察KYSE410、KYSE510和YES2細(xì)胞攝取熒光染料標(biāo)記的KYSE410外泌體;采用Transwell小室實驗分析3種食管癌細(xì)胞的遷移及侵襲能力以及KYSE410外泌體對3種食管癌細(xì)胞遷移及侵襲能力的影響;Western blotting檢測Wnt/β-catenin和PI3K/Akt信號通路相關(guān)蛋白的變化。結(jié)果透射電鏡下觀察KYSE410外泌體具有膜結(jié)構(gòu),直徑分布在30~100nm;3種食管癌細(xì)胞的遷移及侵襲能力從大到小依次為KYSE410、KYSE510、YES2;KYSE410外泌體能夠促進KYSE410、KYSE510、YES2細(xì)胞遷移及侵襲能力并增強3種食管癌細(xì)胞中β-catenin和p-Akt的表達(dá)。結(jié)論來源于高遷移及侵襲能力的食管癌KYSE410細(xì)胞的外泌體能夠促進自身和低遷移及侵襲能力的食管癌KYSE510、YES2細(xì)胞的遷移和侵襲,并且可能通過激活Wnt/β-catenin和PI3K/Akt信號通路發(fā)揮上述作用。
[Abstract]:Objective to investigate the effect of exocrine derived from esophageal carcinoma KYSE410 cells on the migration and invasion ability of tumor cells and its mechanism. Methods the exocrine from the supernatant of esophageal carcinoma KYSE410 cells was isolated by ultracentrifugation. The morphology of exocrine and its markers were observed by transmission electron microscopy and Western blotting, and the KYSE410 exosomes of KYSE410KYSE510 and YES2 cells were observed by confocal microscope. The migration and invasion ability of three kinds of esophageal cancer cells and the effect of KYSE410 exosomes on the migration and invasion of three esophageal cancer cells were analyzed by Transwell chamber assay. The changes of Wnt/ 尾 -catenin and PI3K/Akt signaling pathway related proteins were detected by Western blotting. The membrane structure of KYSE410 exocrine was observed under radio microscope. The migration and invasion ability of three kinds of esophageal carcinoma cells distributed in the diameter of 30 ~ 100nmm ~ (-1) is KYSE410Nm ~ (10) KYSE _ 510N ~ (10) KYSE _ 510A KYSE410 exosome can promote the migration and invasion ability of KYSE410KYSE510nmm2 cells and enhance the expression of 尾 -catenin and p-Akt in three kinds of esophageal carcinoma cells. The exocrine of esophageal carcinoma KYSE410 cells with migration and invasion ability can promote the migration and invasion of KYSE510YES2 cells with low migration and invasion ability. These effects may be played by activating Wnt/ 尾-catenin and PI3K/Akt signaling pathways.
【作者單位】： 國家癌癥中心/中國醫(yī)學(xué)科學(xué)院北京協(xié)和醫(yī)學(xué)院腫瘤醫(yī)院分子腫瘤學(xué)國家重點實驗室;
【基金】：國家自然科學(xué)基金(81572842、81672459) 中國醫(yī)學(xué)科學(xué)院醫(yī)學(xué)與健康科技創(chuàng)新工程項目(2016-12M-1-001)~~
【分類號】：R743.3

【參考文獻】

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