發(fā)布時間：2018-03-28 12:34

  本文選題：宮頸癌　切入點：Oct4　出處：《青島大學(xué)》2017年碩士論文

【摘要】：目的宮頸癌組織中Nanog與Oct4表達及其臨床意義。方法選取江蘇省淮安市婦幼保健院婦科2015年5月-2016年4月期間收治的宮頸癌患者40例作為觀察組研究對象,選取同期我院收治的宮頸上皮內(nèi)瘤變III級(cervical intraepithelial neoplasia,CIN III)患者30例,因子宮肌瘤患者行子宮切除術(shù)獲得的正常宮頸組織10例為研究對照組。采用SP免疫組化染色法檢測宮頸癌組織、CINIII組織及正常宮頸組織中Nanog、Oct4的表達情況,分析宮頸癌組織中Nanog、Oct4表達與疾病臨床病理特征間存在的關(guān)系,進行Nanog與Oct4表達之間相關(guān)性的分析。結(jié)果(1)免疫組化染色法檢測結(jié)果顯示,Nanog與Oct4在宮頸組織中均有表達,Nanog陽性表達呈現(xiàn)點狀,在細胞核上少有灶狀聚集,多數(shù)存在于間質(zhì)和癌巢交界的邊緣。Oct4陽性表達則呈現(xiàn)棕黃色顆粒狀,多表達于細胞核。通過對比發(fā)現(xiàn),宮頸癌組織中Nanog與Oct4的陽性表達率分別為92.50%、92.50%,而CIN組織中陽性表達率分別為70.00%、76.67%,正常宮頸組織中的陽性表達率最低,僅為20.00%、20.00%,三組兩兩比較,均存在統(tǒng)計學(xué)差異(P0.05)。(2)Nanog表達量在宮頸癌組織、CIN組織及正常宮頸組織中分別為(49.57±15.24)×102、(32.31±4.85)×102及(8.46±1.28)×102,三者的表達量IOD比較存在統(tǒng)計學(xué)差異(P0.05);Oct4的表達量IOD在宮頸癌組織、CINIII組織及正常宮頸組織中分別為(15.84±3.25)、(10.39±2.98)及(1.35±0.52)三者的表達量IOD比較存在統(tǒng)計學(xué)差異(P0.05)。(3)探討宮頸癌組織中Nanog與Oct4的表達情況與疾病病理參數(shù)之間的關(guān)系發(fā)現(xiàn),Nanog與Oct4的表達水平同臨床分期和淋巴結(jié)轉(zhuǎn)移之間無相關(guān)性(P0.05);Nanog與Oct4的表達水平在宮頸癌高分化、低分化、中分化組織中存在顯著性差異,兩者的表達水平均與腫瘤的分化程度呈現(xiàn)負相關(guān)(P0.05)。(4)對宮頸癌組織中Oct4及Nanog表達情況采用Spearman等級相關(guān)分析,結(jié)果表明兩者在宮頸癌組織中的表達呈現(xiàn)正相關(guān)性(rs=0.5315,P=0.0026)。結(jié)論在宮頸癌組織中,Nanog與Oct4均存在過量表達;Nanog與Oct4的表達水平與宮頸癌組織的分化程度呈現(xiàn)負相關(guān),分化程度越高,表達水平越低;Nanog與Oct4的表達水平與宮頸癌組織臨床分期和淋巴結(jié)轉(zhuǎn)移無相關(guān)性;宮頸癌組織中Nanog與Oct4的高表達之間存在正相關(guān)關(guān)系。
[Abstract]:Objective to investigate the expression of Nanog and Oct4 in cervical carcinoma and its clinical significance. Methods 40 patients with cervical cancer admitted from May 2015 to April 2016 in Huaian City Maternal and Child Health Hospital in Jiangsu Province were selected as the observation group. Thirty patients with cervical intraepithelial neoplasia (III grade III cervical intraepithelial neoplasia neoplasia cin III) treated in our hospital during the same period were selected. The expression of Nanog-Oct4 in cervical carcinoma tissue (CINIII) and normal cervix cervix was detected by SP immunohistochemical staining. 10 cases of normal cervix cervix tissues obtained by hysterectomy were used as the control group, and the expression of Nanog-Oct4 in cervical carcinoma tissues was detected by SP immunohistochemical staining. To analyze the relationship between the expression of Nanog-Oct4 and the clinicopathological features of cervical cancer. The correlation between Nanog and Oct4 expression was analyzed. Results 1) Immunohistochemical staining showed that the positive expression of Nanog and Oct4 in cervical tissues were punctate, and there were few focal aggregations in the nucleus. The majority of the positive expression of .Oct4 at the edge of the stroma and the edge of the cancer nest was brown granular, mostly expressed in the nucleus. The positive expression rates of Nanog and Oct4 in cervical cancer tissues were 92.50 and 92.50, respectively, while those in CIN tissues were 70.000.76. 677.The positive rates of normal cervix tissues were the lowest, 20.000.00 and 20.000.00, respectively. There was statistical difference in the expression of P0.05, P0.05, P0. 05 ~ (2 +) Nanog in cin and normal cervix tissues, respectively, which were 49.57 鹵15.24 脳 10 ~ 2 脳 10 ~ 2 脳 10 ~ 2 and 8.46 鹵1.28 脳 10 ~ 2 脳 10 ~ 2, respectively. There was a significant difference in IOD expression of IOD in cervical carcinoma tissue and normal cervix. There was a statistical difference among them in the expression of P0.05OT4 in cervical carcinoma tissue and normal cervix tissue. The expression levels of IOD in cervical tissues were 15.84 鹵3.25 (10.39 鹵2.98) and 1.35 鹵0.52 (respectively). There was a statistical difference between the expression of Nanog and Oct4 in cervical carcinoma tissues (P0.05) and the relationship between the expression of Nanog and Oct4 and the pathological parameters of cervical cancer. It was found that the expression levels of Nanog and Oct4 were related to the clinical stages of cervical cancer. There was no correlation between the expression of P0.05 and Oct4 and lymph node metastasis in cervical carcinoma. The expression of Oct4 and Nanog was negatively correlated with the degree of tumor differentiation. Spearman grade correlation analysis was used to analyze the expression of Oct4 and Nanog in cervical carcinoma. The results showed that there was a positive correlation between the expression of Oct4 and Oct4 in cervical carcinoma tissues. Conclusion the overexpression of Oct4 and Oct4 in cervical carcinoma tissues were negatively correlated with the differentiation degree of cervical carcinoma tissues, and the higher the degree of differentiation was, the higher the degree of differentiation was. There was no correlation between the expression level of Nanog and Oct4 and the clinical stage and lymph node metastasis of cervical cancer, but there was a positive correlation between the high expression of Nanog and Oct4 in cervical carcinoma.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R737.33

【參考文獻】

相關(guān)期刊論文 前10條

1 蔡文娟;郭麗萍;葉靖坤;鄭麗嬌;王麗婷;;Nanog、Oct4與子宮內(nèi)膜異位癥的研究進展[J];實用醫(yī)學(xué)雜志;2015年18期

2 Huai?Wu Lu;Jing Li;Yun?Yun Liu;Chang?Hao Liu;Guo?Cai Xu;Ling?Ling Xie;Miao?Fang Wu;Zhong?Qiu Lin;;Can radical parametrectomy be omitted in occult cervical cancer after extrafascial hysterectomy?[J];Chinese Journal of Cancer;2015年09期

3 Yanchun Wang;Shan Hu;Xuemei Hu;Jianjun Li;Yaqi Shen;Xiaoyu Liu;Zhi Wang;Xiaoyan Meng;Zhen Li;Daoyu Hu;;Intravoxel incoherent motion magnetic resonance imaging for diagnosis of cervical cancer and evaluation of response of uterine cervical cancer to radiochemotherapy: A pilot study[J];Oncology and Translational Medicine;2015年04期

4 YU Xiao-li;ZHAO Xiao-e;WANG Hua-yan;MA Bao-hua;;Expression patterns of OCT4, NANOG, and SOX2 in goat preimplantation embryos from in vivo and in vitro[J];Journal of Integrative Agriculture;2015年07期

5 楊征宇;劉德純;;OCT4、SOX2和NANOG在膽囊癌中的表達及臨床意義[J];河南科技大學(xué)學(xué)報(醫(yī)學(xué)版);2015年01期

6 單紫龍;干曉蓉;;Nanog在腫瘤細胞中表達的Meta分析[J];價值工程;2015年07期

7 YANG Yi;LANG Jing He;WANG You Fang;CHENG Xue Mei;CAI Yu Pin;LI Hui;ZHU Bao Li;ZHANG Rui Fen;;Assessing the Effectiveness of a Cervical Cancer Screening Program in a Hospital-based Study[J];Biomedical and Environmental Sciences;2015年01期

8 丁妍;王治校;王小莉;郭興榮;袁雅紅;李成林;李東升;;Nanog過表達對宮頸癌細胞分化程度的影響[J];湖北醫(yī)藥學(xué)院學(xué)報;2014年06期

9 王菊;熊承良;李紅剛;王苗;趙凱;周宗瑤;;宮頸癌HeLa細胞株GCNF和Oct-4誘導(dǎo)分化表達[J];中華腫瘤防治雜志;2014年15期

10 鄧彥飛;劉慶友;鄧�，�;羅嬋;楊素芳;石德順;;水牛Oct4、Nanog基因的克隆及其在水牛胎兒成纖維細胞中的表達[J];中國農(nóng)業(yè)科學(xué);2014年02期

相關(guān)博士學(xué)位論文 前4條

1 陳錦鵬;胰腺癌干細胞Oct4、Nanog基因生物學(xué)特性的研究[D];蘇州大學(xué);2013年

2 馬衣努爾·買提托合提;上皮間質(zhì)化（EMT）在宮頸癌發(fā)生發(fā)展中誘導(dǎo)腫瘤干細胞的作用及其機制研究[D];華中科技大學(xué);2013年

3 劉東擘;宮頸癌細胞中Oct4及PinX1基因的表達調(diào)控機制研究[D];第三軍醫(yī)大學(xué);2012年

4 潘光錦;多能性基因-Oct4，Nanog維持胚胎干細胞多能性機制的研究[D];清華大學(xué);2005年

相關(guān)碩士學(xué)位論文 前10條

1 姜新娣;Oct4、Sox2、Nanog在舌鱗狀細胞癌中的表達及臨床意義[D];南昌大學(xué)醫(yī)學(xué)院;2015年

2 熱孜宛古麗·約麥爾;宮頸癌發(fā)生及HPV感染與腫瘤干細胞標(biāo)記物表達調(diào)控的關(guān)系研究[D];新疆醫(yī)科大學(xué);2015年

3 劉瑩瑩;Foxm1對Nanog基因的調(diào)控作用[D];西北農(nóng)林科技大學(xué);2014年

4 左超;MACC1和OCT4在子宮內(nèi)膜樣腺癌中的表達及臨床意義[D];吉林大學(xué);2014年

5 閆鳳潔;OCT4在SKOV3和SKOV3/DDP卵巢癌細胞中的表達差異分析[D];河北醫(yī)科大學(xué);2014年

6 張璐;干性基因Oct-4和SOX-2在宮頸癌中的表達[D];吉林大學(xué);2014年

7 王苗;生殖細胞核因子和OCT-4在宮頸癌中的表達研究[D];石河子大學(xué);2013年

8 王海景;SOX-2及OCT-4在卵巢漿液性囊腺癌中的表達及臨床意義[D];南昌大學(xué)醫(yī)學(xué)院;2013年

9 單海燕;沉默Oct4、Nanog基因抑制胰腺癌干細胞干性特性的研究[D];南通大學(xué);2012年

10 張明;肝細胞癌中OCT4、SOX2和NANOG的表達及其臨床意義[D];河北醫(yī)科大學(xué);2012年

，

本文編號：1676348