本文選題：長鏈非編碼RNA　切入點：食管鱗癌　出處：《現(xiàn)代腫瘤醫(yī)學(xué)》2017年15期

【摘要】：目的:篩查及分析食管鱗癌中長鏈非編碼RNA(lncRNA)及mRNA的差異表達譜,以及這些差異lncRNAs的功能、互作分子。方法:通過對3對食管鱗癌/癌旁組織的基因芯片篩選及生物信息學(xué)分析,預(yù)測這些差異表達的lncRNAs潛在靶點、通路,及其與臨近編碼基因、轉(zhuǎn)錄因子的相關(guān)性。結(jié)果:按FC值≥2.0且P值≤0.05的標(biāo)準(zhǔn),共篩選得到癌及癌旁組織差異表達的lncRNA 182個,mRNA 108個,GO聚類和KEGG分析發(fā)現(xiàn)差異lncRNAs潛在靶標(biāo)基因與細(xì)胞周期調(diào)控、細(xì)胞分化、細(xì)胞外基質(zhì)等活性密切相關(guān),進一步生物分析得到lncRNA NONHSAT015779、NONHSAT08197、NONHSAT112918和NONHSAT115190等17個lncRNAs可能與食管鱗癌發(fā)生發(fā)展密切相關(guān)。結(jié)論:與癌旁組織相比,lncRNAs在食管鱗癌組織中顯示不同的表達模式,這些差異表達的lncRNAs可能在食管鱗癌發(fā)生和發(fā)展中發(fā)揮重要作用。
[Abstract]:Objective: to screen and analyze the differential expression profiles of long chain noncoding RNAs and mRNA in esophageal squamous cell carcinoma, and the function and interaction of these lncRNAs. Methods: three pairs of esophageal squamous cell carcinoma / paracancerous tissues were screened by microarray and analyzed by bioinformatics. Potential targets, pathways, and their correlation with adjacent coding genes and transcription factors were predicted for these differentially expressed lncRNAs. Results: according to the criteria of FC 鈮，

本文編號：1655174