本文選題：卡波西肉瘤　切入點：基質(zhì)交感分子1(STIM1)　出處：《石河子大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:檢測卡波西肉瘤組織中基質(zhì)交感分子1(STIM1)mRNA和蛋白的表達(dá),分析其表達(dá)與卡波西肉瘤臨床病理特征的關(guān)系,初步闡述STIM1在卡波西肉瘤發(fā)生發(fā)展中的作用,為進(jìn)一步尋找卡波西肉瘤的新的治療靶點提供必要的理論基礎(chǔ)和實驗依據(jù)。方法:采用SYBR Green實時熒光定量PCR和免疫組化EnVision兩步法對卡波西肉瘤組織、血管瘤組織和正常皮膚組織的石蠟組織標(biāo)本進(jìn)行STIM1的mMRA和蛋白表達(dá)水平檢測,觀察三種組織中該基因表達(dá)的差異,并分析該基因表達(dá)水平與卡波西肉瘤斑片期、斑塊期和結(jié)節(jié)期的相關(guān)性,比較與卡波西肉瘤患者不同的性別、年齡、民族、皮損面積、HHV-8感染和HIV感染分組之間的關(guān)系。運(yùn)用SPSS17.0軟件對STIM1的表達(dá)數(shù)據(jù)進(jìn)行統(tǒng)計學(xué)分析。結(jié)果:1.34例卡波西肉瘤組織中,男性29例,女性5例;年齡27~79歲,平均(56.55±16.07)歲;維吾爾族30例,其他民族(哈薩克族和漢族)4例。病程0.5個月到240個月。經(jīng)典型卡波西肉瘤24例,艾滋病相關(guān)型卡波西肉瘤10例,皮損面積≤5%22例,皮損面積5%12例。斑片期5例,斑塊期11例,結(jié)節(jié)期18例。2.STIM1 mRNA在卡波西肉瘤組織、血管瘤組織和正常皮膚組織中的相對表達(dá)量分別為(1.290±1.215),(1.546±1.106)和(1.321±0.964),三種組織兩兩比較,表達(dá)均無明顯差異(p0.05)�？úㄎ魅饬鼋M織中STIM1的mRNA表達(dá)水平在不同性別、民族、人皰疹病毒8型(HHV-8)感染、人類免疫缺陷病毒(HIV)感染、皮損面積及病理分期間,差異均無統(tǒng)計學(xué)意義(p0.05)。3.STIM1蛋白在卡波西肉瘤、血管瘤和正常皮膚組織中表達(dá)陽性率分別為79.41%,81.81%和13.33%;卡波西肉瘤組和血管瘤組的表達(dá)水平差異無統(tǒng)計學(xué)意義(x2=0.049,p0.05),但兩者與正常皮膚組織相比,表達(dá)水平均顯著增高(p0.000);卡波西肉瘤組織中STIM1的蛋白表達(dá)水平在不同的病理分期間存在差異,結(jié)節(jié)期高于斑片期(Z=-2.323,p=0.02)和斑塊期(Z=0.068,p0.05),但在不同性別、民族、人皰疹病毒8型(HHV-8)感染、人類免疫缺陷病毒(HIV)感染、皮損面積間差異均無統(tǒng)計學(xué)意義(p0.05)。HIV合并HHV-8雙陽性和HIV合并HHV-8雙陰性的患者中STIM1蛋白的表達(dá)水平也無明顯統(tǒng)計學(xué)意義(Z=-0.444,p0.05)。4.卡波西肉瘤組織中STIM1 mRNA的表達(dá)水平與病理分期差異無統(tǒng)計學(xué)意義(F=0.289,p=0.751);而STIM1蛋白的表達(dá)水平與病理分期呈正相關(guān)(r=0.611,Z=0.000),進(jìn)一步分析STIM1 mRNA與蛋白的表達(dá)水平為負(fù)相關(guān)關(guān)系(r=-0.058)。結(jié)論:STIM1在卡波西肉瘤組織中均有不同程度的表達(dá),mRNA的表達(dá)水平與正常組織相比無明顯差異,而蛋白呈明顯的高表達(dá),初步表明STIM1的轉(zhuǎn)錄后調(diào)節(jié)可能在卡波西肉瘤發(fā)生發(fā)展中起重要作用。STIM1蛋白的表達(dá)水平在卡波西肉瘤組織和血管瘤組織中無明顯差異,與卡波西肉瘤組織的病理分期呈正相關(guān),結(jié)節(jié)期蛋白的表達(dá)水平最高,而結(jié)節(jié)期的血管增生最為明顯,表明STIM1可能促進(jìn)卡波西肉瘤血管的增生。
[Abstract]:Objective: to detect the expression of matrix sympathetic molecule 1(STIM1)mRNA and protein in Kaposi's sarcoma, to analyze the relationship between the expression and clinicopathological features of Kaposi's sarcoma, and to explore the role of STIM1 in the development of Kaposi's sarcoma. Methods: SYBR Green real-time fluorescence quantitative PCR and immunohistochemical EnVision method were used to detect Kaposi's sarcoma tissues in order to find new therapeutic targets for Kaposi's sarcoma. The mMRA and protein expression levels of STIM1 were detected in paraffin tissues of hemangioma and normal skin tissues. The difference of the expression of mMRA and protein between the three tissues was observed, and the expression level of the gene was compared with that of Kaposi's sarcoma. Correlation between plaque stage and nodular stage. Sex, age, nationality of patients with Kaposi's sarcoma were compared. The relationship between HHV-8 infection and HIV infection was analyzed by SPSS17.0 software. Results among 1.34 cases of Kaposi's sarcoma, 29 cases were male, 5 cases were female, the age was 2779 years (mean 56.55 鹵16.07) years; There were 30 cases of Uygur nationality and 4 cases of other nationalities (Kazak and Han nationality). The course of disease ranged from 0.5 months to 240 months. There were 24 cases of classical Kaposi sarcoma, 10 cases of AIDS-associated Kaposi sarcoma, and 5 cases of skin lesion area 鈮，

本文編號：1652341