本文選題：-氟尿嘧啶　切入點：骨髓基質(zhì)細胞　出處：《中國實驗血液學雜志》2017年04期 　論文類型：期刊論文

【摘要】：目的:探討抑瘤濃度下的5-氟尿嘧啶(5-fluorouracil,5-FU)對人骨髓基質(zhì)細胞是否有損傷作用以及該作用對造血細胞的影響。方法:應(yīng)用CCK-8法測定乳腺癌細胞株MCF-7、結(jié)腸癌細胞株HCT-116及人骨髓基質(zhì)細胞株HS-5對不同濃度5-FU的敏感性。5-FU作用HS-5后,結(jié)晶紫染色計數(shù)成纖維細胞集落;流式細胞術(shù)分析細胞周期;Annexin V/PI雙染及Hoechest染色檢測細胞凋亡;DCFH-DA法檢測胞內(nèi)活性氧(reactive oxygen species,ROS)水平;ELISA及免疫熒光法檢測細胞因子KL、GM-CSF、RANTS、SDF水平。人臍血單個核細胞(human umbilical cord blood mononuclear cell,h UCB-M NC)與HS-5共培養(yǎng)后,臺盼藍染色計數(shù)h UCB-M NC;流式細胞術(shù)檢測細胞周期、ROS水平、CD34+細胞百分率;酶學法檢測谷胱甘肽過氧化物酶(Glutathione peroxidase,GSH-Px)和超氧化物歧化酶(Superoxide dismutase,SOD)含量;β-半乳糖苷酶染色檢測衰老的h UCB-MNC。結(jié)果:5-FU 12.5-100μg/ml對M CF-7、HCT-116和HS-5均有增殖抑制作用,且該作用具有濃度依賴性和時間依賴性,其中HS-5對5-FU更為敏感。5-FU作用后HS-5細胞周期阻滯,凋亡率上升,胞內(nèi)ROS含量顯著升高,造血生長因子分泌降低,炎性趨化因子升高。與經(jīng)5-FU作用的HS-5共培養(yǎng)后,h UCB-MNC數(shù)量及CD34+細胞比例降低,G1期阻滯,細胞抗氧化能力降低,胞內(nèi)ROS含量顯著上升,衰老的造血細胞增多。結(jié)論:5-FU可導(dǎo)致骨髓基質(zhì)細胞氧化損傷、分泌生物活性物質(zhì)改變,誘發(fā)造血細胞氧化應(yīng)激性早衰。
[Abstract]:Objective: to investigate whether 5-fluorouraciline 5-FU (5-fluorouraciline 5-FU) can damage human bone marrow stromal cells (BMSCs) and the effect of 5-fluorouraciline 5-FU (5-FU) on hematopoietic cells. Methods: breast cancer cell line MCF-7, colon cancer cell line HCT-116, and colon cancer cell line HCT-116 were determined by CCK-8 assay. The sensitivity of human bone marrow stromal cell line HS-5 to different concentrations of 5-FU. 5-FU treated HS-5. The colony of fibroblasts was counted by crystal violet staining. Flow cytometry analysis of cell cycle Annexin V/PI double staining and Hoechest staining for detection of apoptosis; Detection of intracellular reactive oxygen species (Ros) by DCFH-DA; Elisa; immunofluorescence assay; detection of the level of cytokine KLGM-CSFRANTSU SDF. Human umbilical cord blood mononuclear cells in human umbilical cord blood mononuclear cells. UCB-M NCC) co-cultured with HS-5, Trypan blue staining counted h UCB-M NC, and flow cytometry was used to detect the percentage of CD34 cells at Ros level. The contents of glutathione peroxidase (Glutathione peroxidase) and superoxide dismutase (SOD) and 尾 -galactosidase (尾 -galactosidase) were detected by enzymatic method. Results the proliferation of MCF-7HCT-116 and HS-5 was inhibited by 5-FU 12.5-100 渭 g/ml. The effect was concentration-dependent and time-dependent. HS-5 was more sensitive to 5-FU. 5-FU could block the cell cycle, increase the apoptosis rate, increase the content of intracellular ROS and decrease the secretion of hematopoietic growth factor. The number of UCB-MNC and the proportion of CD34 cells decreased in G 1 phase after co-culture with 5-FU, and the antioxidant ability of cells decreased, and the content of intracellular ROS increased significantly after co-culture with 5-FU. Conclusion 5-FU can induce oxidative damage of bone marrow stromal cells, secretion of bioactive substances, and induce oxidative stress premature senescence of hematopoietic cells.
【作者單位】： 重慶醫(yī)科大學干細胞與組織工程學研究室組織學胚胎學教研室;
【基金】：國家自然科學基金資助項目(81173398) 重慶市科委基礎(chǔ)與前沿研究資助項目(cstc2014jcyj A10001)
【分類號】：R730.5

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