本文選題：口腔鱗狀細(xì)胞癌　切入點(diǎn)：HHLA2　出處：《安徽醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：研究目的:口腔鱗狀細(xì)胞癌的發(fā)生發(fā)展是人體內(nèi)多種因素相互作用的復(fù)雜的病理過程,其中免疫炎癥微環(huán)境的改變是重要的環(huán)節(jié),并且免疫炎癥微環(huán)境變化貫穿于發(fā)生發(fā)展過程的始終,即由黏膜到異常增生再到原位癌。HHLA2(B7H7/B7-H5)是最新發(fā)現(xiàn)的B7家族的成員,在許多正常組織中不表達(dá),而在腫瘤中呈現(xiàn)高表達(dá),并且表現(xiàn)與腫瘤的淋巴結(jié)轉(zhuǎn)移有一定相關(guān)性。本文通過免疫組化研究并分析HHLA2及體內(nèi)相關(guān)免疫炎癥指標(biāo)的變化,分析相互間相關(guān)性,探討其在OSCC發(fā)生發(fā)展過程中的可能作用機(jī)制,評測其作為病理診斷的輔助診斷的應(yīng)用價(jià)值。研究方法:選取41例口腔鱗狀細(xì)胞癌、8例異常增生和9例正�？谇火つそM織,借助組織微陣列免疫組織化學(xué)技術(shù)分析口腔正常黏膜組織,口腔異常增生組及口腔鱗狀細(xì)胞癌中HHLA-2,PD-L1,PD-L2,細(xì)胞因子Il-6,M2型巨噬細(xì)胞標(biāo)記物(CD163,CD206),T細(xì)胞(CD4,CD8),EGFR蛋白的表達(dá)情況,從而探討其在口腔異常增生及口腔鱗狀細(xì)胞癌中的相關(guān)性及其表達(dá)意義,進(jìn)一步明確其在口腔鱗狀細(xì)胞癌發(fā)病機(jī)制的影響,為口腔鱗狀細(xì)胞癌的早期診斷提供參考。結(jié)果:(1)HHLA-2,IL-6在正常黏膜、口腔異常增生,口腔鱗狀細(xì)胞癌中表達(dá)率依次增高,而正常黏膜和口腔鱗狀細(xì)胞癌組間陽性率具有統(tǒng)計(jì)學(xué)差異(P0.05),且淋巴結(jié)轉(zhuǎn)移組相對于未轉(zhuǎn)移組表達(dá)率差異有明顯統(tǒng)計(jì)學(xué)差異(P0.05)。(2)PD-L1,CD206和CD8在正常黏膜、口腔異常增生,口腔鱗狀細(xì)胞癌中表達(dá)率依次增高,但表達(dá)率并不具有統(tǒng)計(jì)學(xué)意義。PD-L2,CD-4,CD206在正常黏膜、口腔異常增生,口腔鱗狀細(xì)胞癌中表達(dá)率依次增高,口腔異常增生和口腔鱗狀細(xì)胞癌相對于正常黏膜,表達(dá)量具有統(tǒng)計(jì)學(xué)差異(P0.05)。(3)EGFR在口腔鱗狀細(xì)胞癌中表達(dá)量相對于正常黏膜,表達(dá)量具有統(tǒng)計(jì)學(xué)差異(P0.05)。CD163,CD4在正常黏膜、口腔異常增生,口腔鱗狀細(xì)胞癌中表達(dá)率依次增高,口腔異常增生和口腔鱗狀細(xì)胞癌相對于正常黏膜,表達(dá)量具有統(tǒng)計(jì)學(xué)差異(P0.05),且淋巴結(jié)轉(zhuǎn)移組相對于未轉(zhuǎn)移組表達(dá)率差異有明顯統(tǒng)計(jì)學(xué)差異(P0.05)。(4)HHLA-2的表達(dá)量與PD-L1,PD-L2,M2巨噬細(xì)胞(CD163,CD206),IL-6的表達(dá)呈正相關(guān)。(5)PD-L1,PD-L2分別與EGFR正相關(guān),而EGFR與CD4,CD8負(fù)相關(guān)。結(jié)論:HHLA-2,M2型巨噬細(xì)胞與PD-L1.PD-L2在口腔鱗狀細(xì)胞癌的發(fā)生發(fā)展中起著一定作用。HHLA-2、PD-L1、PD-L2具有抑制CD4,CD8細(xì)胞增殖作用,可能由于抑制了EGFR受體。HHLA-2與PD-L1,PD-L2,M2型巨噬細(xì)胞表達(dá)呈正相關(guān),可能在口腔黏膜發(fā)展到口腔癌這一過程中具有協(xié)同作用.
[Abstract]:Objective: the occurrence and development of oral squamous cell carcinoma (OSCC) is a complicated pathological process of interaction of many factors in human body, in which the change of immune inflammation microenvironment is an important link. And the changes of immune inflammation microenvironment throughout the process of occurrence and development, that is, from mucous membrane to abnormal proliferation to cancer in situ. H2HLA-B7H7 / B7-H) is a member of the newly discovered B7 family, which is not expressed in many normal tissues, but is highly expressed in tumors. The expression was correlated with lymph node metastasis. The changes of HHLA2 and related immune inflammation indexes in vivo were analyzed by immunohistochemistry, and the correlation between them was analyzed. To explore the possible mechanism of OSCC and evaluate its application value as auxiliary diagnosis of pathological diagnosis. Methods: 41 cases of oral squamous cell carcinoma (OSCC) with abnormal hyperplasia and 9 cases of normal oral mucosal tissue were selected. The expression of HHLA-2PD-L1pPD-L1 PD-L2 and the cytokine Il-6M2-type macrophage marker, CD163C / CD206 ~ (+) T cell, in normal oral mucosa, dysplasia of oral cavity and oral squamous cell carcinoma (OSCC) were analyzed by microarray immunohistochemical technique, and the expression of CD4 / CD8EGFR protein was detected in normal oral mucosa, dysplasia of oral cavity and oral squamous cell carcinoma (OSCC). To explore the correlation and significance of its expression in oral dysplasia and oral squamous cell carcinoma (OSCC), and to further clarify its role in the pathogenesis of oral squamous cell carcinoma (OSCC). Results in normal mucosa, abnormal proliferation of oral cavity and oral squamous cell carcinoma, the expression rate of HHLA-2C6 in oral squamous cell carcinoma increased in turn. The positive rates of CD206 and CD8 in normal mucosa and oral squamous cell carcinoma group were significantly different compared with those in non-metastasis group (P 0.05), and the expression of CD206 and CD8 in normal mucosa and oral cavity were abnormal in lymph node metastasis group. In oral squamous cell carcinoma, the expression rate increased in turn, but the expression rate was not statistically significant in normal mucosa, oral dysplasia, oral squamous cell carcinoma and oral squamous cell carcinoma. Compared with the normal mucosa, the expression of P0.05U. 3EGFR in oral squamous cell carcinoma and oral squamous cell carcinoma was significantly different from that in normal mucosa. The expression level of P0.05 + CD163T CD4 in normal mucosa was significantly different from that in normal mucosa, and the expression of CD163T CD4 in oral squamous cell carcinoma was significantly higher than that in normal mucosa, and the expression of CD163T CD4 in oral squamous cell carcinoma was significantly higher than that in oral squamous cell carcinoma. The expression rate of oral squamous cell carcinoma (OSCC) increased in turn, and the abnormal proliferation of oral cavity and oral squamous cell carcinoma (OSCC) were relative to normal mucosa. The expression of HHLA-2 in lymph node metastasis group was significantly different from that in non-metastasis group. The expression of HHLA-2 in lymph node metastasis group was positively correlated with the expression of EGFR in macrophages of PD-L1 and PD-L2M2.The expression of IL-6 was positively correlated with the expression of CD163mCD206, and the expression of PD-L1PD-L2 was positively correlated with the expression of EGFR in the lymph node metastasis group, and the positive correlation was found between the expression of HHLA-2 in the lymph node metastasis group and that in the macrophage of PD-L1 and PD-L2m-2 macrophages. Conclusion EGFR and PD-L1.PD-L2 play a role in the development of oral squamous cell carcinoma. HHLA-2pPD-L1PD-L2 can inhibit the proliferation of CD4 / CD8 cells, which may be due to the inhibition of the expression of EGFR receptor. HHLA-2 and PD-L1 / M 2 macrophages are positively correlated with the expression of PD-L1PD-L2 type M 2 macrophages. May have a synergistic effect in the development of oral mucosa to oral cancer.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R739.8

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相關(guān)期刊論文 前1條

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本文編號：1637086