本文選題：賁門癌　切入點(diǎn)：Rictor　出處：《河南科技大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:檢測(cè)Rictor及Fox O1基因在賁門癌組織及對(duì)應(yīng)癌旁組織中的表達(dá)差異,分析其與賁門癌臨床病理特征之間的關(guān)系,判斷其作為賁門癌早期診斷的可行性。方法:于2012年2月到2013年10月收集30例河南科技大學(xué)第一附屬醫(yī)院病理確診并行賁門癌根治術(shù)的賁門癌患者,取其癌組織及對(duì)應(yīng)癌旁組織,所有病例術(shù)前未經(jīng)放療和化療。(1)賁門癌組織中Rictor及Fox O1基因的m RNA檢測(cè)設(shè)計(jì)并合成了檢測(cè)Rictor及Fox O1基因的引物,運(yùn)用RT-PCR方法對(duì)賁門癌和對(duì)應(yīng)癌旁組織中的Rictor及Fox O1 m RNA表達(dá)進(jìn)行檢測(cè)。(2)賁門癌組織中Rictor及Fox O1基因的蛋白檢測(cè)運(yùn)用免疫組化和Western blot的方法對(duì)賁門癌及其對(duì)應(yīng)癌旁組織中Rictor及Fox O1基因的蛋白表達(dá)進(jìn)行檢測(cè)。運(yùn)用SPSS17.0統(tǒng)計(jì)學(xué)軟件對(duì)數(shù)據(jù)進(jìn)行統(tǒng)計(jì)學(xué)處理,分析Rictor及Fox O1的表達(dá)與賁門癌患者的臨床病理特征的關(guān)系。結(jié)果:(1)RT-PCR結(jié)果顯示,在m RNA水平Rictor分子的表達(dá)在賁門癌組織及對(duì)應(yīng)癌旁組織中均呈陽(yáng)性,其中26例在賁門癌組織中的表達(dá)低于賁門癌對(duì)應(yīng)癌旁組織中的表達(dá)(26/30),占86.7%,4例在賁門癌組織中的表達(dá)高于賁門癌相應(yīng)癌旁組織中的表達(dá)(4/30),占13.3%,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。在m RNA水平Fox O1分子的表達(dá)在30例賁門癌組織及其對(duì)應(yīng)癌旁組織中的表達(dá)均呈陽(yáng)性,其中有25例在賁門癌組織中的表達(dá)低于賁門癌對(duì)應(yīng)癌旁組織中的表達(dá)(25/30),占83.3%,5例在賁門癌組織中的表達(dá)高于對(duì)應(yīng)癌旁組織中的表達(dá)(5/30),占16.7%,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。(2)免疫組化結(jié)果顯示,在蛋白水平Rictor分子的表達(dá)在賁門癌組織及對(duì)應(yīng)癌旁組織中的陽(yáng)性率分別為46.7%(14/30)、76.7%(23/30),差異具有統(tǒng)計(jì)學(xué)意義(P0.05);相對(duì)于在對(duì)應(yīng)癌旁組織中的表達(dá),賁門癌組織中Rictor低表達(dá)與患者的年齡、性別、TNM分期及淋巴結(jié)轉(zhuǎn)移均無(wú)關(guān)(P0.05),而與腫瘤分化程度密切相關(guān)(P0.05)。Fox O1蛋白在賁門癌組織、對(duì)應(yīng)癌旁組織中的陽(yáng)性率分別為53.3%(16/30)、86.7%(26/30),差異具有統(tǒng)計(jì)學(xué)意義(P0.05);相對(duì)于在對(duì)應(yīng)癌旁組織中的表達(dá),賁門癌組織中Fox O1低表達(dá)與患者的年齡、性別、腫瘤分化程度及淋巴結(jié)轉(zhuǎn)移均無(wú)關(guān)(P0.05),與腫瘤TNM分期相關(guān)(P0.05)。(3)Western blot結(jié)果顯示,Rictor蛋白在賁門癌組織及其對(duì)應(yīng)癌旁組織中均呈陽(yáng)性表達(dá),其中24例在賁門癌組織中的表達(dá)低于賁門癌對(duì)應(yīng)癌旁組織中的表達(dá)(24/30),占80.0%;6例在賁門癌組織的表達(dá)高于賁門癌對(duì)應(yīng)癌旁組織中的表達(dá)(6/30),占20.0%,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。Fox O1蛋白在賁門癌組織及其對(duì)應(yīng)癌旁組織中均呈陽(yáng)性表達(dá),其中24例在賁門癌組織中的表達(dá)低于賁門癌對(duì)應(yīng)癌旁組織中的表達(dá)(24/30)占80.0%;6例在賁門癌組織中的表達(dá)高于賁門癌對(duì)應(yīng)癌旁組織中的表達(dá)(6/30),占20.0%,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。(4)分別對(duì)Rictor和Fox O1的Western blot、RT-PCR結(jié)果進(jìn)行一致性分析,結(jié)果為:r=0.609和r=0.429。說(shuō)明Rictor和Fox O1蛋白表達(dá)和m RNA表達(dá)是一致的。(5)對(duì)Rictor和Fox O1進(jìn)行相關(guān)性分析,Western blot及RT-PCR結(jié)果相關(guān)系數(shù)分別為:r=0.429和r=0.870。說(shuō)明Rictor和Fox O1呈正相關(guān)。結(jié)論:(1)Rictor在賁門癌組織中的表達(dá)低于其在對(duì)應(yīng)癌旁組織中的表達(dá),提示Rictor可能參與了賁門癌發(fā)生、發(fā)展和侵襲轉(zhuǎn)移的過(guò)程。(2)Fox O1在賁門癌組織中的表達(dá)低于其在對(duì)應(yīng)癌旁組織中的表達(dá),提示Fox O1可能與賁門癌的進(jìn)程和轉(zhuǎn)移相關(guān),從側(cè)面應(yīng)證了賁門癌癌旁組織中Fox O1的高表達(dá)可能是賁門黏膜發(fā)生癌變的重要過(guò)程。(3)Rictor和Fox O1的表達(dá)呈正相關(guān),可能是在PI3K/AKT/m TOR信號(hào)傳導(dǎo)通路中,上游基因Rictor激活了下游基因Fox O1的表達(dá),提示Rictor和Fox O1基因可能在賁門癌的發(fā)生發(fā)展中起著協(xié)同作用。
[Abstract]:Objective: to detect Rictor and Fox expression differences of O1 gene in gastric cancer tissues and corresponding adjacent to the analysis of its relationship with the clinicopathological features of cardiac carcinoma, as judge the feasibility of early diagnosis of cardiac cancer. Methods: from February 2012 to October 2013 collected 30 cases of the First Affiliated Hospital of Henan University of Science and Technology of pathological diagnosis of parallel radical resection of cardiac carcinoma in patients with cardia cancer, the cancer tissues and corresponding adjacent tissues, all cases without preoperative radiotherapy and chemotherapy. (1) m RNA Rictor and Fox O1 gene detection of cardiac carcinoma and synthesized primers to detect Rictor and Fox of O1 gene, using RT-PCR method to detect the expression of cardiac cancer and the tumor tissues of Rictor Fox and O1 m RNA. (2) was detected by immunohistochemistry and Western blot Rictor and Fox O1 gene in gastric cardiac carcinoma of cardia cancer and non cancerous To detect the expression of Rictor and Fox O1 gene proteins in the tissues. Using SPSS17.0 statistical software for statistical processing of data, the relationship between the clinicopathological features and expression analysis of Rictor Fox O1 and cardiac cancer patients. Results: (1) the results of RT-PCR showed that the expression level of RNA in M of Rictor molecules in the tissue of cardiac cancer and corresponding tumor tissues showed positive expression, the expression in 26 cases of cardiac carcinoma and cardia cancer below the tumor tissues (26/30), accounting for 86.7%, 4 cases expressed in cardiac carcinoma tissues was higher than that of cardia cancer adjacent tissue of the expression group (4/30), accounting for 13.3%, with statistical significant differences (P0.05). The expression of M RNA level Fox O1 molecule expression in 30 cases of cardia carcinoma and corresponding adjacent tissues were positive, the expression in 25 cases of cardiac carcinoma and cardia cancer below the tumor tissues (25/ 30), accounting for 83.3%, 5 cases of expression in gastric cardia carcinoma was higher than that of the corresponding tumor adjacent tissues (5/30), accounting for 16.7%, the difference was statistically significant (P0.05). (2) the results of immunohistochemistry showed that the positive rate of expression of Rictor at the protein level of molecules in cardiac cancer tissues and corresponding adjacent tissues were 46.7% (14/30), 76.7% (23/30), the difference was statistically significant (P0.05); compared with the expression in adjacent to the low expression of Rictor in gastric cardia carcinoma patients with age, gender, TNM stage and lymph node metastasis (P0.05), which is closely related with the tumor the degree of differentiation (P0.05) of.Fox O1 protein in cardiac carcinoma tissues, the positive rate of the tumor tissues were 53.3% (16/30), 86.7% (26/30), the difference was statistically significant (P0.05); relative to the organization in adjacent in the expression of Fox and low expression of O1 in patients with gastric cardia carcinoma age sex, The degree of tumor differentiation and lymph node metastasis (P0.05), associated with the stage of tumor TNM (P0.05). (3) Western blot results showed that the positive expression of Rictor protein in cardiac carcinoma tissues and corresponding adjacent tissues, the expression of the expression in 24 cases of cardiac carcinoma and cardia carcinoma adjacent below in the organization (24/30), accounting for 80%; the expression in 6 cases of cardiac cancer was higher than that of cardia cancer the tumor tissues (6/30), accounting for 20%, the difference was statistically significant (P0.05).Fox O1 protein group were positive in the fabric in the tissue of cardiac cancer and corresponding para carcinoma, the expression of expression in 24 cases of cardiac carcinoma and cardia cancer below the tumor tissues (24/30) accounted for 80%; the expression in 6 cases of cardiac carcinoma and cardia cancer than the tumor tissues (6/30), accounting for 20%, the difference was statistically significant (P0.05). (4) respectively Rictor and F Ox O1 Western blot RT-PCR, the consistency analysis, results are as follows: r=0.609 and r=0.429. Rictor and expression of Fox O1 protein and M Expression of RNA is consistent. (5) to analyze the correlation between the Rictor and Fox O1, Western blot and RT-PCR the correlation coefficient were r= 0.429 and r=0.870. Rictor and positively Fox O1. Conclusion: (1) the expression of Rictor in gastric cardia carcinoma tissues than that in adjacent tissues and the expression of Rictor may be involved in the process of cardia cancer, development and metastasis of Fox. (2) the expression of O1 in gastric cardia carcinoma is lower than its expression in adjacent tissues the Fox O1 may be associated with cardiac carcinoma and metastasis process, from the side should permit a high expression of gastric cardia adenocarcinoma tissues Fox O1 may be an important process in the cardia mucosa carcinogenesis. (3) the expression of Rictor was positively related to O1 and Fox, may be in P In the I3K/AKT/m TOR signaling pathway, the upstream gene Rictor activated the downstream gene Fox O1 expression, suggesting that Rictor and Fox O1 gene may play a synergistic role in the occurrence and development of gastric cardia cancer.

【學(xué)位授予單位】：河南科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R735.2

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