發(fā)布時間：2018-03-11 09:11

  本文選題：分泌型卷曲相關(guān)蛋白　切入點(diǎn)：肺腺癌　出處：《腫瘤防治研究》2017年05期 　論文類型：期刊論文

【摘要】：目的探討SFRP1基因甲基化及其蛋白在肺腺癌組織中的表達(dá)。方法收集手術(shù)切除的肺腺癌標(biāo)本60例和癌旁組織30例,采用甲基化特異性PCR法、實(shí)時熒光定量PCR法和免疫組織化學(xué)染色法分別檢測SFPR1基因甲基化、m RNA及蛋白的表達(dá)情況。結(jié)果 SFRP1基因甲基化率在肺腺癌中(58.3%)表達(dá)明顯高于癌旁組織(20.0%)(P=0.001)。SFRP1 m RNA在肺腺癌(0.847±0.297)中的表達(dá)明顯低于癌旁組織(2.019±0.445)(P0.01)。SFRP1蛋白在肺腺癌中的陽性表達(dá)率為38.3%(23/60),明顯低于癌旁組織中的表達(dá)率83.3%(25/30)(P0.001);SFRP1蛋白表達(dá)與肺腺癌組織分化程度、臨床分期及淋巴結(jié)轉(zhuǎn)移情況有關(guān)(均P0.05)。SFRP1基因甲基化與其蛋白表達(dá)缺失存在相關(guān)性(r=-0.585,P0.001)。結(jié)論 SFRP1蛋白的表達(dá)降低可能是由于SFRP1基因甲基化所致,SFRP1基因甲基化及其蛋白表達(dá)與肺腺癌組織分化程度及淋巴結(jié)轉(zhuǎn)移情況密切相關(guān)。
[Abstract]:Objective to investigate the expression of SFRP1 gene methylation and its protein in lung adenocarcinoma. Methods 60 cases of lung adenocarcinoma and 30 cases of adjacent tissues were collected by methylation specific PCR. The expression of methylated m RNA and protein of SFPR1 gene was detected by real-time fluorescence quantitative PCR and immunohistochemical staining. Results the methylation rate of SFRP1 gene in lung adenocarcinoma was significantly higher than that in paracancerous tissue (P 0.001). SFRP1 m RNA was higher in lung adenocarcinoma than that in paracancerous tissue. The expression of SFRP1 protein in adenocarcinoma was significantly lower than that in paracancerous tissue (2.019 鹵0.445), and the positive rate of SFRP1 protein in lung adenocarcinoma was 38.3%. It was significantly lower than that in paracancerous tissue (83.3% 30%) and the degree of differentiation of lung adenocarcinoma, and the expression of SFRP1 protein was significantly lower than that in paracancerous tissue (P 0.001). Clinical stage and lymph node metastasis were related to the methylation of SFRP1 gene and its protein deletion. Conclusion the decreased expression of SFRP1 protein may be due to the methylation of SFRP1 gene and its protein. The expression was closely related to the degree of differentiation and lymph node metastasis of lung adenocarcinoma.
【作者單位】： 南方醫(yī)科大學(xué)附屬武漢臨床醫(yī)學(xué)院;中國人民解放軍武漢總醫(yī)院心胸外科;
【基金】：中國博士后科學(xué)基金(201150M1579)
【分類號】：R734.2

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