本文選題：光譜學(xué)　切入點(diǎn)：表面增強(qiáng)拉曼散射　出處：《鄭州大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：胃癌是常見惡性腫瘤之一,南美、東歐和東亞是胃癌的高發(fā)地區(qū),全世界每年有70多萬人死于胃癌。在中國胃癌的發(fā)病率位居各類腫瘤第三,發(fā)病有明顯的地域性差異,西北青海地區(qū)與東部沿海地區(qū)胃癌發(fā)病率相比南方廣西廣東地區(qū)明顯高出許多,男女發(fā)病比例近似為2:1,好發(fā)年齡在54歲以上。雖然胃癌發(fā)病率頗高,但鑒別起來還是比較困難,常規(guī)的胃癌診斷過程包含體檢,組織檢查和影像學(xué)檢查,但這些方法都存在著浸入性、耗時等特點(diǎn),當(dāng)確診是胃癌為時已晚。從而早期診斷對胃癌患者的治療具有重要意義,本文采用表面增強(qiáng)拉曼光譜技術(shù)和紫外-可見吸收光譜技術(shù)對胃癌患者和健康人的血液樣本進(jìn)行分析和統(tǒng)計。結(jié)果顯示,紫外-可見吸收光譜所包含的信息量太少,很難對健康人和胃癌患者的血液樣品進(jìn)行很好區(qū)分。而血紅蛋白拉曼光譜是在血氧飽和度定量測定,血液代用品的高鐵血紅蛋白含量檢測及疾病狀態(tài)下異常血紅蛋白檢測中優(yōu)勢都較為明顯。本文采用微波加熱方法制備銀膠體粒子,該方法簡單、快捷。分別對20例胃癌患者和11例健康人的血紅蛋白進(jìn)行表面增強(qiáng)拉曼散射,得到重復(fù)性很高和特征峰突出的拉曼譜。通過SERS譜峰歸屬分析顯示胃癌患者血紅蛋白中的吡咯環(huán)、酪氨酸和苯丙氨酸都低于正常人。對血紅素結(jié)構(gòu)進(jìn)行分析與討論,當(dāng)血紅蛋白與氧結(jié)合之后,Fe2+離子處于低自旋態(tài),離子半徑因縮小而位移了0.75?從而落入血紅素卟啉環(huán)平面中央的孔隙之中。這種空間變化使與鐵相連的F8 His受到牽動,會使珠蛋白中的兩股螺旋之間空隙縮小,導(dǎo)致將HC2酪氨酸排擠出空隙。利用這一機(jī)理,通過1560 cm-1的吸收峰進(jìn)一步證實(shí)胃癌患者酪氨酸含量低于正常人。結(jié)合主成分分析(PCA)的方法,對所得的拉曼散射光譜建立多元統(tǒng)計模型并進(jìn)行分析與統(tǒng)計,得到的光譜峰值的降維三維散點(diǎn)圖,這張圖可以非常好的區(qū)分健康人與胃癌患者。利用判別分析方法得出該算法診斷胃癌的靈敏度和特異性分別為90.0%和90.9%,總判別正確率為90.3%。研究結(jié)果表明,氧合血紅蛋白表面增強(qiáng)拉曼(SERS)的光譜統(tǒng)計分析是可以作為一種新型胃癌的診斷方法。
[Abstract]:Gastric cancer is one of the most common malignant tumors. The incidence of gastric cancer is high in South America, Eastern Europe and East Asia. More than 700,000 people die of gastric cancer every year in the world. The incidence of gastric cancer in China ranks third among all kinds of tumors, and there are significant regional differences in the incidence of gastric cancer. The incidence of gastric cancer in northwest Qinghai and eastern coastal areas is much higher than that in southern Guangxi and Guangdong. The incidence ratio of male and female is approximately 2: 1, and the predilection age is over 54. Although the incidence of gastric cancer is quite high, it is still difficult to distinguish. The routine diagnosis of gastric cancer includes physical examination, tissue examination and imaging examination, but these methods have the characteristics of immersion, time consuming and so on. When the diagnosis of gastric cancer is too late, the early diagnosis is of great significance for the treatment of gastric cancer patients. Surface enhanced Raman spectroscopy and UV-Vis absorption spectroscopy were used to analyze and statistics the blood samples of patients with gastric cancer and healthy people. The results showed that the UV-Vis absorption spectra contained too little information. It is difficult to distinguish the blood samples of healthy people from those with gastric cancer, and the hemoglobin Raman spectroscopy is measured quantitatively by oxygen saturation. The advantages of methemoglobin content in blood substitutes and abnormal hemoglobin in the condition of disease are obvious. In this paper, silver colloid particles were prepared by microwave heating. Fast. Surface enhanced Raman scattering of hemoglobin in 20 patients with gastric cancer and 11 healthy persons was carried out, and the Raman spectra with high reproducibility and prominent characteristic peaks were obtained. The pyrrole ring in hemoglobin of gastric cancer patients was revealed by SERS spectrum analysis. Both tyrosine and phenylalanine are lower than normal people. The heme structure is analyzed and discussed. When hemoglobin is combined with oxygen, Fe _ 2 ion is in a low spin state, and the ionic radius is shifted from 0.75 to 0.75? This spatial change in the center of the heme porphyrin ring affects the iron-linked F8 His, which reduces the gap between the two helices in globin, leading to the extrusion of HC2 tyrosine out of the void. The absorption peak of 1560 cm-1 further confirmed that the tyrosine content of gastric cancer patients was lower than that of normal persons. The reduced dimension 3D scatter plot of the peak value of the spectrum is obtained, The sensitivity and specificity of the algorithm for the diagnosis of gastric cancer were 90.0% and 90.9, respectively, and the total discriminant accuracy was 90.3%. Spectral statistical analysis of surface enhanced Raman SERS is a new diagnostic method for gastric cancer.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R735.2;O657.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前5條

1 謝濟(jì)運(yùn),蔣治良,鐘福新,馮忠偉,郭秀姣;血細(xì)胞的光譜特性研究[J];分析測試技術(shù)與儀器;2000年03期

2 郝雅瓊,吳玉清,劉俊秋,任曉君,羅貴民;半胱氨酸在銀基底表面吸附機(jī)理的拉曼光譜研究[J];光散射學(xué)報;2002年03期

3 涂麗云;鄒永p，

本文編號：1590770