本文關(guān)鍵詞： 原發(fā)性肝癌 乙型肝炎病毒 基因型 篩查　出處：《廣西醫(yī)科大學》2017年碩士論文　論文類型：學位論文

【摘要】：目的:探討廣西貴港地區(qū)防癌篩查對象的乙型肝炎病毒(HBV)感染情況和HBV基因型的分布特征,為開展肝癌早診早治項目工作提供依據(jù)。方法:整理并分析貴港三個區(qū)(港南區(qū)、港北區(qū)、覃塘區(qū))30~59歲居民接受肝癌篩查的數(shù)據(jù),本次共有150124人接受篩查,其中HBsAg陽性者有18005人,從中對1989例HBsAg陽性者的血清進行HBV DNA定量檢測,以選取HBV DNA病毒拷貝數(shù)大于檢出限(1×103)的HBs Ag攜帶者,進一步運用聚合酶鏈反應(yīng)-限制性片段長度多態(tài)性法(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)進行HBV基因分型。結(jié)果:篩查人群中HBsAg陽性率為11.99%,男性HBsAg陽性率高于女性(P0.01),不同年齡組HBsAg陽性率差異有統(tǒng)計學意義(P0.01),HBs Ag陽性率隨著年齡的增長而呈下降趨勢。在18005例肝癌高危人群中,AFP陽性414例,陽性率為2.30%;共發(fā)現(xiàn)58例肝癌新病例,其中早期肝癌為13例,早期診斷率為22.41%,中晚期肝癌為45例。在肝癌高危人群中,HBV DNA病毒拷貝數(shù)≥1×103為755例,其中HBV A基因型6例(0.79%),B基因型337例(44.64%),C基因型293例(38.81%),D基因型18例(2.39%),UT(Untypable)基因型101例(13.37%),B和C基因型在男女性中的分布無統(tǒng)計學意義(P均0.05);各年齡組間HBV基因型的分布差異無統(tǒng)計學意義(P均0.05)。結(jié)論:廣西貴港三個區(qū)肝癌篩查人群的HBV感染率為11.99%,HBV基因型以B和C型為主。
[Abstract]:Objective: to investigate the hepatitis B virus hepatitis B virus (HBV) infection and the distribution characteristics of HBV genotypes among the cancer prevention screening subjects in Guigang, Guangxi, so as to provide the basis for the project of early diagnosis and early treatment of liver cancer. A total of 150124 people were screened for liver cancer at the age of 59 years in Qantang District, Qantang District, and 18005 of them were HBsAg positive. HBV DNA was quantitatively detected in the sera of 1989 HBsAg positive patients. In order to select HBs Ag carriers with HBV DNA virus copy number greater than the detection limit of 1 脳 103. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLPs) was further used to type HBV gene. Results: the positive rate of HBsAg was 11.99 in the screening population. The positive rate of HBsAg in males was higher than that in females, and HBsAg was positive in different age groups. The positive rate of HBs Ag decreased with the increase of age. In 18005 cases of high risk population for liver cancer, 414 cases were positive for AFP. The positive rate was 2.30%, 58 new cases of HCC were found, including 13 cases of early HCC, 22.41 cases of early diagnosis and 45 cases of advanced HCC. In the high risk population of HCC, the copy number of HBV DNA virus 鈮，

本文編號：1501432