本文關(guān)鍵詞： 結(jié)直腸癌 單核苷酸多態(tài)性 生存預(yù)后 c-Jun基因　出處：《實(shí)用醫(yī)學(xué)雜志》2017年21期 　論文類型：期刊論文

【摘要】：目的探討c-Jun基因啟動(dòng)子多態(tài)性位點(diǎn)rs4646999與腸癌預(yù)后的相關(guān)性。方法采用Taq-Man探針?lè)z測(cè)436例腸癌患者rs4646999-673CT的基因型,通過(guò)Kaplan-Meier法和Cox回歸分析其生存曲線;采用免疫印跡分析多態(tài)性位點(diǎn)不同基因型的c-Jun蛋白表達(dá)水平。結(jié)果單因素分析表明攜帶TT基因型患者累積生存率顯著高于CT和CC基因型攜帶者,差異有統(tǒng)計(jì)學(xué)意義。多因素Cox回歸分析顯示分化程度、淋巴結(jié)轉(zhuǎn)移、遠(yuǎn)處轉(zhuǎn)移、TNM分期和rs4646999位點(diǎn)基因型為預(yù)后因素,CT/CC基因型與腸癌較差的生存預(yù)后相關(guān)(P0.05)。蛋白表達(dá)分析發(fā)現(xiàn)CC基因型癌組織c-Jun蛋白表達(dá)升高,而TT基因型則相反。結(jié)論 c-Jun基因啟動(dòng)子區(qū)域多態(tài)性位點(diǎn)rs4646999與腸癌生存預(yù)后相關(guān),其作用機(jī)制可能是增強(qiáng)c-Jun蛋白表達(dá)導(dǎo)致腸癌發(fā)生發(fā)展。
[Abstract]:Objective to investigate the correlation between c-Jun gene promoter polymorphism rs4646999 and prognosis of colorectal cancer. Methods rs464699 was detected by Taq-Man probe method in 436 patients with colorectal cancer. The genotype of 9-673CT. The survival curve was analyzed by Kaplan-Meier method and Cox regression analysis. Results univariate analysis showed that the cumulative survival rate of patients with TT genotype was significantly higher than that of patients with CT and CC genotypes. Multivariate Cox regression analysis showed that the degree of differentiation, lymph node metastasis, distant metastasis, TNM staging and rs4646999 locus genotype were prognostic factors. CT/CC genotypes were associated with poor survival and prognosis of colorectal cancer. Protein expression analysis showed that c-Jun protein expression was increased in CC genotype carcinoma. Conclusion the polymorphism of c-Jun gene promoter region rs4646999 is associated with the survival and prognosis of colorectal cancer. Its mechanism may be to enhance the expression of c-Jun protein, leading to the carcinogenesis and development of colorectal cancer.
【作者單位】： 中山大學(xué)附屬第六醫(yī)院腫瘤內(nèi)科;中山大學(xué)附屬第六醫(yī)院結(jié)直腸外科;廣東省結(jié)直腸盆底疾病研究重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金青年項(xiàng)目(編號(hào):81201581) 廣東省自然科學(xué)基金面上項(xiàng)目(編號(hào):S2013010013478) 廣州市科技計(jì)劃項(xiàng)目(編號(hào):201707010430)
【分類號(hào)】：R735.3
【正文快照】： 結(jié)直腸癌是最常見的惡性腫瘤之一,目前已躍居常見腫瘤第三位,全球每年新發(fā)病例近120萬(wàn)、死亡人數(shù)達(dá)60萬(wàn)[1]。在中國(guó)結(jié)直腸癌的發(fā)病率呈逐漸上升的趨勢(shì),尤其近年更以4%速度迅猛增長(zhǎng),嚴(yán)重威脅人們的健康和生命[2]。由于結(jié)直腸癌早期癥狀不明顯,無(wú)特異性,多數(shù)患者在初診 時(shí)已處

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