本文關鍵詞： 胃癌 GPR124 臨床意義　出處：《南華大學》2015年碩士論文　論文類型：學位論文

【摘要】：目的:檢測GPR124在胃癌組織中的表達情況,分析其臨床病理意義,為胃癌的早期診斷和分子靶向藥物治療提供有價值的參考。方法:收集20例胃癌、癌旁組織和正常組織的新鮮標本,提取組織蛋白和總RNA,分別運用Western blot和RT-PCR檢測GPR124蛋白和RNA在上述不同組織中的表達情況。同時收集不同分化腺癌、非典型增生、正常胃組織石蠟標本共99例,運用免疫組織化學染色檢測不同胃組織中GPR124蛋白的陽性率。分析GPR124蛋白在不同胃組織中表達的差別及意義。結(jié)果:1、Western blot檢測結(jié)果:GPR124蛋白在胃癌組織中的表達明顯高于癌旁組織和正常組織(P0.05),癌旁組織也高于正常組織(P0.05)。2、RT-PCR檢測結(jié)果:GPR124在胃癌組織中的表達明顯高于癌旁組織和正常組織(P0.05),癌旁組織也高于正常組織(P0.05)。3、免疫組化染色檢測結(jié)果:GPR124蛋白主要表達于胞膜,在胃癌組織、非典型增生組織、正常組織的陽性表達率分別為77.78%(49/63)、30.77%(4/13)、4.35%(1/23)。GPR124蛋白在癌組織中的陽性表達率顯著高于非典型增生組織和正常組織(P0.05),非典型增生組織的陽性表達率表達高于正常組織(P0.05);另外,GPR124蛋白在高分化、中分化、低分化胃癌組織中的陽性表達率分別為33.33%(4/12)、75.00%(15/20)、96.77%(30/31),三組比較均有統(tǒng)計學意義(P0.05);III~IV期胃癌的陽性表達率明顯高于I~II期,伴淋巴結(jié)轉(zhuǎn)移胃癌的陽性表達率也明顯高于無轉(zhuǎn)移者。結(jié)論:1、GPR124蛋白在胃癌組織中表達上調(diào)。2、GPR124蛋白的表達水平與胃癌的分化程度、臨床病理分期及是否伴淋巴結(jié)轉(zhuǎn)移有關。
[Abstract]:Objective: to detect the expression of GPR124 in gastric cancer and analyze its clinicopathological significance, and to provide valuable reference for early diagnosis and molecular targeted drug therapy of gastric cancer. Methods: 20 cases of gastric cancer were collected. Tissue proteins and total RNA were extracted from fresh specimens of paracancerous tissues and normal tissues. Western blot and RT-PCR were used to detect the expression of GPR124 protein and RNA in different tissues, and different differentiated adenocarcinoma and atypical hyperplasia were collected. There were 99 cases of paraffin wax in normal gastric tissue. Immunohistochemical staining was used to detect the positive rate of GPR124 protein in different gastric tissues. The difference and significance of the expression of GPR124 protein in different gastric tissues were analyzed. Results: 1. The results of Western blot analysis showed that the expression of GPR124 protein in gastric cancer was significantly higher than that in paracancerous tissues and normal tissues (P0.05). The expression of TGPR124 in gastric cancer tissues was significantly higher than that in adjacent tissues and normal tissues (P0.05). The results of immunohistochemical staining showed that the protein of 1% GPR124 was mainly expressed in the cell membrane, and in gastric cancer tissue and atypical hyperplasia tissue. The positive expression rates in normal tissues were 77.78% and 30.77%, respectively. The positive expression rate of GPR124 protein in cancer tissues was significantly higher than that in atypical hyperplasia tissues and normal tissues (P0.05). The positive expression rate in atypical hyperplasia tissues was higher than that in normal tissues. In addition, the positive expression rates of GPR124 protein in well-differentiated, moderately differentiated and poorly differentiated gastric cancer tissues were 33.33 / 12 / 75.00 / 20 respectively. 96.7777% 30% 31%, all the three groups had statistical significance (P 0.05). The positive expression rate of gastric cancer in III~IV stage was significantly higher than that in stage II, and the positive expression rate of gastric carcinoma with lymph node metastasis was significantly higher than that in patients without metastasis. Conclusion: 1. The expression of GPR124 protein in gastric carcinoma was related to the degree of differentiation, clinicopathologic stage and lymph node metastasis.
【學位授予單位】：南華大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R735.2

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