本文關(guān)鍵詞： 低劑量輻射 卵巢癌 耐藥 ERCC1 Bcl-2　出處：《青島大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:卵巢癌是所有女性惡性腫瘤中容易引起死亡的疾病。而卵巢癌的主要治療方法是手術(shù)治療加上術(shù)后的多藥聯(lián)合化療,其中鉑類是多藥聯(lián)合化療中的基礎(chǔ)藥物。然而對(duì)于卵巢癌來說,其主要的挑戰(zhàn)來自癌細(xì)胞對(duì)于化療藥物抵抗性的發(fā)生。所以本試驗(yàn)的目的在于證實(shí)低劑量輻射有增強(qiáng)耐藥卵巢癌細(xì)胞化療敏感性的作用,并探究其機(jī)制,然后為耐藥性卵巢癌的臨床治療提供新的策略。方法:CCK8法檢測(cè)細(xì)胞增殖。采用流式細(xì)胞儀(FCM)的方法,通過Annexin V-FITC和PI的雙染色來量化耐順鉑卵巢癌細(xì)胞(SKOV3/DDP)的凋亡率。實(shí)時(shí)熒光定量PCR(q PCR)分析SKOV3/DDP細(xì)胞中ERCC1和Bcl-2的m RNA表達(dá)水平。結(jié)果:對(duì)照組,常規(guī)劑量組和低劑量組的IC50值分別為9.367±0.16,9.289±0.16和3.847±0.15分別(P0.05)。經(jīng)過流式細(xì)胞儀檢測(cè),低劑量輻射組較其他組具有更顯著的凋亡率(p0.05)。低劑量組的ERCC1和Bcl-2的m RNA相對(duì)表達(dá)量比對(duì)照組及常規(guī)劑量組顯著降低(p0.05)。結(jié)論:低劑量輻射具有增加SKOV3/DDP細(xì)胞對(duì)化療藥物(順鉑)敏感性的作用,而其這一作用可能是通過減弱SKOV3/DDP細(xì)胞的DNA損傷修復(fù)能力和加強(qiáng)腫瘤細(xì)胞的凋亡來實(shí)現(xiàn)的。
[Abstract]:Objective: ovarian cancer is one of the leading causes of death in all female malignancies. The main treatment for ovarian cancer is surgical treatment plus postoperative multidrug chemotherapy. Among them, platinum is the basic drug in multidrug combination chemotherapy. However, for ovarian cancer. The main challenge is the development of cancer cell resistance to chemotherapeutic drugs, so the aim of this study is to confirm that low dose radiation enhances chemosensitivity of drug-resistant ovarian cancer cells and to explore its mechanism. Then it provided a new strategy for the clinical treatment of drug-resistant ovarian cancer. Methods the cell proliferation was detected by the method of: CCK8. Flow cytometry (FCM) was used. The apoptosis rate of cisplatin resistant ovarian cancer cell line SKOV3 / DDP was quantified by double staining of Annexin V-FITC and Pi. Real-time fluorescence quantitative PCR(q). The expression of m RNA in ERCC1 and Bcl-2 in SKOV3/DDP cells was analyzed. Results: control group. The IC50 values of routine and low dose groups were 9.367 鹵0.16, respectively. 9.289 鹵0.16 and 3.847 鹵0.15, respectively. The relative expression of m RNA in ERCC1 and Bcl-2 in the low dose group was significantly lower than that in the control group and the normal dose group (P < 0. 05), and the relative expression of m RNA in the low dose group was significantly lower than that in the control group and the normal dose group (P < 0. 05). Conclusion: low dose radiation can increase the sensitivity of SKOV3/DDP cells to chemotherapeutic drugs (cisplatin). This effect may be achieved by weakening the DNA damage and repair ability of SKOV3/DDP cells and enhancing the apoptosis of tumor cells.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R737.31

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