本文關(guān)鍵詞：EGFR、PI3K、PTEN在人腦膠質(zhì)瘤中的表達及臨床意義分析　出處：《昆明醫(yī)科大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

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【摘要】：[目的]本研究檢測PI3K信號通路相關(guān)蛋白EGFR、PI3K和PTEN在人腦膠質(zhì)瘤中的表達情況。分析其表達與腫瘤級別、患者臨床指標(biāo)的關(guān)系,以及這幾種蛋白之間的相關(guān)性,探討其臨床意義,以期對人腦膠質(zhì)瘤靶向治療的臨床應(yīng)用提供參考。[方法]收集昆明醫(yī)科大學(xué)第一附屬醫(yī)院2014年1月至2015年6月符合入選條件的人腦膠質(zhì)瘤石蠟標(biāo)本50例。采用免疫組化SP法分別檢測EGFR、PI3K和PTEN蛋白在50例標(biāo)本中的表達,記錄染色強度及陽性細胞率。采用χ2檢驗比較不同級別膠質(zhì)瘤,不同性別、年齡及腫瘤大小中各蛋白的表達差異。采用Spearman等級相關(guān)分析檢驗EGFR,PI3K及PTEN之間相關(guān)性。[結(jié)果]在本研究50例腦膠質(zhì)瘤標(biāo)本中,EGFR蛋白總的陽性率為50%,在低級別膠質(zhì)瘤中陽性率為34.62%,在高級別膠質(zhì)瘤中陽性率為66.67%,差異有統(tǒng)計學(xué)意義(P0.05)。PI3K蛋白總的陽性率為60%,在低級別膠質(zhì)瘤中陽性率為46.15%,在高級別膠質(zhì)瘤中陽性率為75%,差異有統(tǒng)計學(xué)意義(P0.05)。PTEN蛋白總的陽性率為54%,在低級別膠質(zhì)瘤中陽性率為69.23%,在高級別膠質(zhì)瘤中陽性率為37.5%,差異有統(tǒng)計學(xué)意義(P0.05)。EGFR、PI3K和PTEN在不同性別、不同年齡段及不同腫瘤大小中的表達無統(tǒng)計學(xué)差異(P0.05)。EGFR的表達與PI3K的表達呈正相關(guān)(r=0.327,P0.05),PTEN的表達與EGFR,PI3K的表達均呈負相關(guān)(r=-0.281,r=-0.344,P0.05)。[結(jié)論]1.EGFR,PI3K的表達水平隨膠質(zhì)瘤級別上升而增高,PTEN的表達水平隨膠質(zhì)瘤級別上升而下降。2.EGFR、PI3K和PTEN的表達與患者性別、年齡(50歲或≥50歲)及腫瘤大小(5cm或≥5cm)無關(guān)。3.EGFR的表達與PI3K的表達呈正相關(guān),EGFR過表達可能通過激活PI3K信號通路在膠質(zhì)瘤的發(fā)生發(fā)展中起作用。4.PETN的表達與EGFR、PI3K的表達呈負相關(guān),揭示在腦膠質(zhì)瘤中PTEN可能與PI3K信號通路拮抗,PTEN表達下降或缺失與EGFR過表達共同作用于PI3K信號通路,促進膠質(zhì)瘤的發(fā)生及惡性轉(zhuǎn)化。
[Abstract]:[Objective] to detect the expression of PI3K signaling pathway related proteins EGFRRP-PI3K and PTEN in human glioma and to analyze the relationship between the expression of EGFRP-PI3K and tumor grade and clinical index of patients. To explore the clinical significance of these proteins in order to provide reference for the clinical application of targeted therapy for human glioma. [Methods A total of 50 paraffin specimens of human glioma were collected from the first affiliated Hospital of Kunming Medical University from January 2014 to June 2015. EGFR were detected by immunohistochemical SP method. The expression of PI3K and PTEN protein in 50 samples were recorded. The staining intensity and the positive cell rate were recorded. 蠂 2 test was used to compare different grades of gliomas, different genders. Spearman grade correlation analysis was used to test the correlation between EGFR PI3K and PTEN. [Results: the total positive rate of EGFR protein was 50 in 50 gliomas and 34.62% in low grade gliomas. In high grade glioma, the positive rate was 66.67, the difference was statistically significant. The total positive rate of P0.05U. PI3K protein was 60 in high grade glioma, and the positive rate in low grade glioma was 46.15%. In high grade glioma, the positive rate was 75. The total positive rate of P0.05. PTEN protein was 54 and the positive rate of low grade glioma was 69.23%. The positive rate in high grade glioma was 37.5%, the difference was statistically significant (P 0.05). EGFRN PI3K and PTEN were different in sex. There was no significant difference in the expression of P0.05P. EGFR and the expression of PI3K in different age groups and different tumor sizes. There was a positive correlation between the expression of P0.05 and the expression of PI3K. There was a negative correlation between the expression of PTEN and EGFR PI3K. [Conclusions: 1. The expression of PI3K increased with the increase of glioma grade. The expression level of PTEN decreased with the increase of glioma grade. 2. The expression of PI3K and PTEN was not correlated with sex, age 50 years old or 鈮，

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