本文關(guān)鍵詞：胚胎蛋白誘導(dǎo)多種腫瘤細(xì)胞凋亡的比較研究　出處：《東北師范大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 胚胎蛋白 MTT 腫瘤 凋亡 小鼠模型

【摘要】：腫瘤與細(xì)胞的異常分化直接相關(guān)。胚胎發(fā)育,是細(xì)胞不斷增殖、誘導(dǎo)正常細(xì)胞分化、并伴有凋亡的過程,而在這其間出現(xiàn)的異常細(xì)胞分化則會(huì)被誘導(dǎo)修正。胚胎在發(fā)育過程中能夠產(chǎn)生促分化因子,誘導(dǎo)胚胎細(xì)胞分化、凋亡,決定胚胎發(fā)育的方向。近年來促分化因了的應(yīng)用,尤其在誘導(dǎo)腫瘤細(xì)胞凋亡方面,引起了人們的廣泛關(guān)注。經(jīng)本實(shí)驗(yàn)室前期研究發(fā)現(xiàn),動(dòng)物的胚胎蛋白在誘導(dǎo)腫瘤細(xì)胞凋亡方面作用顯著,并且具有普遍性。本文進(jìn)一步驗(yàn)證,胚胎提取蛋白對(duì)于多種腫瘤細(xì)胞的體外培養(yǎng)過程中,均具有抑制細(xì)胞增殖、誘導(dǎo)凋亡的作用,并初步驗(yàn)證胚胎蛋白對(duì)小鼠體內(nèi)腫瘤細(xì)胞同樣具有誘導(dǎo)凋亡的作用。本文選用雞胚胎提取蛋白以及蛙胚胎提取蛋白作為誘導(dǎo)物,以不同濃度梯度分別誘導(dǎo)多同腫瘤細(xì)胞,以比對(duì)其誘導(dǎo)凋亡差異性。選用的腫瘤細(xì)胞主要有：RM1、HepG2、NLT、MCF7、Hela、B16等。通過光學(xué)顯微鏡、電子顯微鏡、激光共聚焦顯微鏡,以及MTT,流式細(xì)胞儀等檢測(cè),證實(shí)胚胎蛋白對(duì)各種腫瘤細(xì)胞均具有抑制增殖、誘導(dǎo)凋亡的效果,且具有誘導(dǎo)物濃度影響誘導(dǎo)效果的差異。將B16細(xì)胞接種到小鼠皮下,建立體內(nèi)腫瘤動(dòng)物模型,采用原位注射法,將胚胎蛋白注入小鼠體內(nèi),結(jié)果證實(shí),在實(shí)驗(yàn)組小鼠體內(nèi),腫瘤的體積,與對(duì)照組相比明顯減小。初步驗(yàn)證了胚胎蛋白對(duì)生長(zhǎng)在小鼠體內(nèi)的腫瘤細(xì)胞同樣具有誘導(dǎo)凋亡的作用。
[Abstract]:Tumor is directly related to abnormal differentiation of cells. Embryonic development is the process of cell proliferation, induction of normal cell differentiation and apoptosis. However, the abnormal cell differentiation will be induced and corrected during the process of embryo development, which can induce differentiation and apoptosis of embryonic cells. In recent years, the application of differentiation promotion, especially in inducing apoptosis of tumor cells, has attracted wide attention. Animal embryonic proteins play a significant role in inducing apoptosis of tumor cells, and they are universal. In this paper, we further verify that embryonic proteins can be used in the culture of various tumor cells in vitro. Both of them can inhibit cell proliferation and induce apoptosis. It was also proved that embryonic protein could induce apoptosis in mouse tumor cells. Chicken embryo extract protein and frog embryo extract protein were selected as inducers. Different concentrations of gradient were used to induce the same tumor cells, and to compare the difference of inducing apoptosis. The main tumor cells selected were: RM1, HepG2, NLTG2NLTMCF7 and Hela. B16 et al. By optical microscope, electron microscope, laser confocal microscope, MTT, flow cytometry and other detection, it was proved that embryonic protein can inhibit the proliferation of various tumor cells. B16 cells were inoculated subcutaneously into mice to establish tumor animal model in vivo, and in situ injection method was used. Embryonic proteins were injected into mice and the results showed that the tumor volume was measured in mice of experimental group. Compared with the control group, it was proved that embryonic protein could induce apoptosis of tumor cells in mice.
【學(xué)位授予單位】：東北師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R73-36

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