本文關(guān)鍵詞：ZEB-1在胃癌組織中的表達(dá)及與胃癌生物學(xué)行為的關(guān)系　出處：《安徽醫(yī)科大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 胃腫瘤 上皮-間質(zhì)轉(zhuǎn)化 ZEB-1 腫瘤侵襲 免疫組織化學(xué)

【摘要】：目的探討胃癌組織中ZEB-1和c-jun蛋白的表達(dá)及對(duì)腫瘤發(fā)生、發(fā)展的影響。建立人胃癌細(xì)胞株BGC-823中ZEB-1基因沉默模型,研究其對(duì)胃癌細(xì)胞增殖、遷移、侵襲的影響及機(jī)制。方法采用免疫組化(IHC)法檢測100例胃癌及癌旁組織中ZEB-1c-jun和E-cadherin的表達(dá)。構(gòu)建沉默ZEB-1基因的si RNA干擾序列,轉(zhuǎn)染到人胃癌細(xì)胞BGC-823,使用RT-PCR檢測轉(zhuǎn)染前后ZEB-1 m RNA表達(dá)的情況,挑選出沉默效率最高的si RNA干擾片段。使用CCK-8法檢測細(xì)胞增殖情況,使用Transwell小室檢測細(xì)胞的遷移和侵襲能力。結(jié)果胃癌組織ZEB-1、c-jun和E-cadherin的陽性表達(dá)率分別為81%、70%、35%,癌旁組織陽性表達(dá)率分別為17%、25%、100%。ZEB-1和c-jun蛋白在胃癌組織中的表達(dá)均顯著高于癌旁組織(P0.05)。ZEB-1陽性表達(dá)與腫瘤浸潤深度、分化程度、淋巴結(jié)轉(zhuǎn)移、TNM分期有關(guān)(P0.05),而與年齡、性別、腫塊大小無明顯相關(guān)性。c-jun陽性表達(dá)與腫瘤分化程度有關(guān)(P0.05),與其它臨床病理特征無明顯相關(guān)。ZEB-1與E-cadherin在胃癌組織中表達(dá)存在負(fù)相關(guān)(P0.05),ZEB-1與c-jun表達(dá)存在正相關(guān)(P0.05)。ZEB-1與c-jun高表達(dá)患者術(shù)后5年生存率明顯低于低表達(dá)患者。經(jīng)RT-PCR檢測,BGC-823細(xì)胞轉(zhuǎn)染干擾si RNA后,ZEB-1-si RNA3表達(dá)在m RNA水平受到抑制最明顯。CCK-8法檢測細(xì)胞增殖能力,實(shí)驗(yàn)組、空白對(duì)照組、陰性對(duì)照組細(xì)胞的增殖能力無明顯差異(P0.05)。細(xì)胞侵襲實(shí)驗(yàn)中,實(shí)驗(yàn)組細(xì)胞穿膜細(xì)胞數(shù)顯著低于空白對(duì)照組和陰性對(duì)照組(P0.05)。細(xì)胞徖移實(shí)驗(yàn)中,實(shí)驗(yàn)組細(xì)胞穿膜細(xì)胞數(shù)顯著低于空白對(duì)照組和陰性對(duì)照組(P0.05)。結(jié)論ZEB-1與c-jun在胃癌中的高表達(dá)與胃癌發(fā)生、發(fā)展密切相關(guān),可作為判斷胃癌預(yù)后的指標(biāo)。ZEB-1在促進(jìn)胃癌的侵襲轉(zhuǎn)移過程中起到重要作用,可以成為控制胃癌浸潤、轉(zhuǎn)移的新靶點(diǎn)。
[Abstract]:Objective to investigate the expression of ZEB-1 and c-jun protein in gastric carcinoma and its effect on the occurrence and development of tumor. To establish the ZEB-1 gene silencing model in human gastric cancer cell line BGC-823, and to study the effect and mechanism of the model on the proliferation, migration and invasion of gastric cancer cells. Methods the expression of ZEB-1? C-jun and E-cadherin in 100 cases of gastric cancer and adjacent tissues were detected by immunohistochemistry (IHC). The Si RNA interference sequence of silencing ZEB-1 gene was constructed and transfected into human gastric cancer cell line BGC-823. The expression of ZEB-1 m RNA before and after transfection was detected by RT-PCR, and the Si RNA interference fragment with the highest silencing efficiency was selected. CCK-8 assay was used to detect cell proliferation, and Transwell cells were used to detect cell migration and invasiveness. Results the positive expression rates of ZEB-1, c-jun and E-cadherin in gastric cancer tissues were 81%, 70% and 35% respectively. The positive expression rates of para cancerous tissues were 17%, 25% and 100%, respectively. The expression of ZEB-1 and c-jun protein in gastric cancer tissues was significantly higher than that of para cancerous tissue (P0.05). The positive expression of ZEB-1 was related to tumor invasion depth, differentiation degree, lymph node metastasis and TNM stage (P0.05), but not related to age, sex and size of tumor. The positive expression of c-jun was associated with the degree of tumor differentiation (P0.05), and had no significant correlation with other clinicopathological features. There was a negative correlation between the expression of ZEB-1 and E-cadherin in gastric cancer tissues (P0.05), and there was a positive correlation between ZEB-1 and c-jun expression (P0.05). The 5 year survival rate of patients with high expression of ZEB-1 and c-jun was significantly lower than that of low expression patients. After RT-PCR detection, BGC-823 cells transfected with Si RNA, the expression of ZEB-1-si RNA3 was most inhibited at the level of M RNA. The proliferation of cells in the experimental group, the blank control group and the negative control group had no significant difference (P0.05) by CCK-8 assay. In the experiment of cell invasion, the number of cell transmembrane cells in the experimental group was significantly lower than that in the blank control group and the negative control group (P0.05). In the experimental group, the number of cell transmembrane cells in the experimental group was significantly lower than that in the blank control group and the negative control group (P0.05). Conclusion the high expression of ZEB-1 and c-jun in gastric cancer is closely related to the occurrence and development of gastric cancer, and can be used as an indicator to judge the prognosis of gastric cancer. ZEB-1 plays an important role in promoting the invasion and metastasis of gastric cancer, and can be a new target to control the invasion and metastasis of gastric cancer.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R735.2

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