電針對骨質(zhì)疏松癥大鼠骨密度和TNF-α、OPG的影響
發(fā)布時間：2018-07-31 20:40
【摘要】：目的：以骨質(zhì)疏松癥大鼠為研究對象,觀察電針療法對骨質(zhì)疏松癥大鼠骨密度、血清TNF-α、OPG的影響,探討電針療法治療骨質(zhì)疏松癥大鼠的機理,為臨床應用電針療法治療骨質(zhì)疏松癥提供科學依據(jù)。方法：40只SD大鼠隨機分為4組,每組10只：空白組(A組)、模型組(B組)、電針組(C組)、藥物組(D組)。所有大鼠在造模之前采用雙能X線測定儀檢測各組大鼠左側(cè)股骨、L4、L5的骨密度�？瞻捉M除外,其余3組均摘除雙側(cè)卵巢行去勢手術,術后喂養(yǎng)90天,后再次采用雙能X線測定儀檢測各組大鼠左側(cè)股骨、L4、L5的骨密度�？瞻捉M及模型組除外,其余2組分別每天予以電針、藥物方法處理4個療程,共計52天。療程結(jié)束后采用雙能X線測定儀檢測各組大鼠左側(cè)股骨、L4、L5的骨密度,取血液采用ELISE法測定血清中TNF-α、OPG的濃度。結(jié)果：1.與空白組比較,模型組大鼠的骨密度值明顯減少,差異有統(tǒng)計學意義(P0.05)；2.與模型組比較,電針組、藥物組大鼠的骨密度值明顯增加,差異有統(tǒng)計學意義(P0.05)；3.與藥物組比較,電針組骨密度值有統(tǒng)計學意義(P0.05)；4.與空白組比較,模型組大鼠的TNF-α值明顯增加,差異有統(tǒng)計學意義(P0.05)；5.與模型組比較,電針組、藥物組大鼠的TNF-α值明顯減少,差異有統(tǒng)計學意義(P0.05)；6.與藥物組比較,電針組大鼠的TNF-α值有統(tǒng)計學意義(P0.05)；7.與空白組相比,模型組大鼠的OPG值明顯減少,差異有統(tǒng)計學意義(P0.05)；8.與模型組比較,電針組、藥物組大鼠的OPG值明顯增加,差異有統(tǒng)計學意義(P0.05)；9.與藥物組比較,電針組大鼠的OPG值有統(tǒng)計學意義(P0.05)。結(jié)論：1.電針治療與藥物治療均能提高骨質(zhì)疏松癥大鼠模型的骨密度值,電針治療提高骨質(zhì)疏松癥大鼠模型的骨密度值效果更佳。2.電針治療與藥物治療均能降低骨質(zhì)疏松癥大鼠模型血清TNF-α濃度,電針治療降低骨質(zhì)疏松癥大鼠模型血清TNF-α濃度效果更佳。3.電針治療與藥物治療均能升高骨質(zhì)疏松癥大鼠模型血清OPG濃度,電針治療升高骨質(zhì)疏松癥大鼠模型血清OPG濃度效果更佳。
[Abstract]:Objective: To observe the effect of electroacupuncture therapy on bone density, serum TNF- alpha and OPG in osteoporotic rats, and to explore the mechanism of electroacupuncture therapy for osteoporotic rats, and to provide a scientific basis for clinical application of electroacupuncture in the treatment of osteoporosis. Methods: 40 rats were randomly divided into 4 groups of 10 rats in each group, 10 rats in each group. The blank group (group A), the model group (group B), the electroacupuncture group (group C) and the drug group (group D). The bone mineral density of the left femur, L4, L5 in all rats was detected by double energy X ray instrument before making the model. The other 3 groups were removed by bilateral ovariectomy and were fed for 90 days after the operation, and then the double energy X ray instrument was used to detect each group again. The bone density of the left femur of the rat, L4, L5 bone density, except in the blank group and the model group, the other 2 groups were treated with electroacupuncture every day and the drug method was treated for 4 courses for a total of 52 days. The bone mineral density of the left femur, L4, L5, and the concentration of TNF- A and OPG in the serum were measured by the double energy X-ray instrument after the course of treatment, and the concentration of TNF- A and OPG in the serum was measured by ELISE. Results: 1. Compared with the blank group, the bone mineral density of the model rats decreased significantly (P0.05). 2. compared with the model group, the bone mineral density of the electroacupuncture group and the drug group increased significantly (P0.05). Compared with the drug group, the bone mineral density value of the electroacupuncture group was statistically significant (P0.05); 4. compared with the blank group, the model was compared with the blank group. The TNF- alpha value of the group rats increased significantly (P0.05). 5. compared with the model group, the TNF- alpha value of the electroacupuncture group and the drug group was significantly decreased, the difference was statistically significant (P0.05). Compared with the drug group, the TNF- alpha value of the electroacupuncture group was statistically significant (P0.05); 7. compared with the blank group, the OPG value of the model group was clear. The difference was statistically significant (P0.05). 8. compared with the model group, the OPG value of the electroacupuncture group and the drug group increased significantly (P0.05). 9. compared with the drug group, the OPG value of the electroacupuncture group was statistically significant (P0.05). Conclusion: 1. electroacupuncture treatment and drug treatment can improve the model of osteoporosis rats. Bone mineral density value, electroacupuncture treatment improved bone mineral density value of osteoporosis rat model better.2. electroacupuncture treatment and drug treatment can reduce the concentration of serum TNF- alpha in osteoporosis rat model, electroacupuncture treatment decreased the concentration effect of TNF- alpha in osteoporosis rat model, better.3. electroacupuncture treatment and drug treatment can increase bone thinning. The serum concentration of OPG in the pine rat model is better than that in the electroacupuncture treatment, which can increase the serum OPG concentration in the osteoporosis rat model.
【學位授予單位】：湖南中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R245;R259
[Abstract]:Objective: To observe the effect of electroacupuncture therapy on bone density, serum TNF- alpha and OPG in osteoporotic rats, and to explore the mechanism of electroacupuncture therapy for osteoporotic rats, and to provide a scientific basis for clinical application of electroacupuncture in the treatment of osteoporosis. Methods: 40 rats were randomly divided into 4 groups of 10 rats in each group, 10 rats in each group. The blank group (group A), the model group (group B), the electroacupuncture group (group C) and the drug group (group D). The bone mineral density of the left femur, L4, L5 in all rats was detected by double energy X ray instrument before making the model. The other 3 groups were removed by bilateral ovariectomy and were fed for 90 days after the operation, and then the double energy X ray instrument was used to detect each group again. The bone density of the left femur of the rat, L4, L5 bone density, except in the blank group and the model group, the other 2 groups were treated with electroacupuncture every day and the drug method was treated for 4 courses for a total of 52 days. The bone mineral density of the left femur, L4, L5, and the concentration of TNF- A and OPG in the serum were measured by the double energy X-ray instrument after the course of treatment, and the concentration of TNF- A and OPG in the serum was measured by ELISE. Results: 1. Compared with the blank group, the bone mineral density of the model rats decreased significantly (P0.05). 2. compared with the model group, the bone mineral density of the electroacupuncture group and the drug group increased significantly (P0.05). Compared with the drug group, the bone mineral density value of the electroacupuncture group was statistically significant (P0.05); 4. compared with the blank group, the model was compared with the blank group. The TNF- alpha value of the group rats increased significantly (P0.05). 5. compared with the model group, the TNF- alpha value of the electroacupuncture group and the drug group was significantly decreased, the difference was statistically significant (P0.05). Compared with the drug group, the TNF- alpha value of the electroacupuncture group was statistically significant (P0.05); 7. compared with the blank group, the OPG value of the model group was clear. The difference was statistically significant (P0.05). 8. compared with the model group, the OPG value of the electroacupuncture group and the drug group increased significantly (P0.05). 9. compared with the drug group, the OPG value of the electroacupuncture group was statistically significant (P0.05). Conclusion: 1. electroacupuncture treatment and drug treatment can improve the model of osteoporosis rats. Bone mineral density value, electroacupuncture treatment improved bone mineral density value of osteoporosis rat model better.2. electroacupuncture treatment and drug treatment can reduce the concentration of serum TNF- alpha in osteoporosis rat model, electroacupuncture treatment decreased the concentration effect of TNF- alpha in osteoporosis rat model, better.3. electroacupuncture treatment and drug treatment can increase bone thinning. The serum concentration of OPG in the pine rat model is better than that in the electroacupuncture treatment, which can increase the serum OPG concentration in the osteoporosis rat model.
【學位授予單位】：湖南中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R245;R259
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相關期刊論文 前10條
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