本文選題：偏頭痛 + 硝酸甘油�。� 參考：《遼寧中醫(yī)藥大學》2013年碩士論文

【摘要】：目的：通過復制皮下注射硝酸甘油偏頭痛大鼠模型，，觀察針刺對偏頭痛大鼠行為學變化的影響及對血清內(nèi)SP、CGRP含量變化的影響，探討針刺預防及治療偏頭痛的機制。 材料與方法： 1.健康雄性SD大鼠50只，體重約為250+50g,適應性喂養(yǎng)一周之后開始實驗。按照隨機數(shù)字表法將大鼠分為5組（空白對照組、模型對照組、針刺預防組、針刺治療組、針刺預防+治療組），每組10只�？瞻讓φ战M：正常飼養(yǎng)7天，不進行任何處理；模型對照組：正常飼養(yǎng)7天，第8天皮下注射硝酸甘油造模；針刺預防組：針刺治療7天，20min/d，第8天皮下注射硝酸甘油造模；針刺治療組：正常飼養(yǎng)7天，第8天皮下注射硝酸甘油造模，造模成功后，針刺20min一次；針刺預防+治療組：針刺治療7天，20min/d，第8天皮下注射硝酸甘油造模，造模成功后，針刺20min一次。 2.皮下注射硝酸甘油（10mg/kg）制備偏頭痛大鼠模型。 3.針刺取穴：百會、陽陵泉（雙側(cè)）、太沖（雙側(cè)）、外關(guān)（雙側(cè)）。 4.觀察造模后大鼠行為學變化，采用以下三項作為觀察指標：①大鼠耳紅持續(xù)時間：觀察并記錄大鼠雙耳發(fā)紅出現(xiàn)和消失的時間點并計算其持續(xù)時間；②大鼠前肢頻繁撓頭行為持續(xù)時間：觀察并記錄大鼠前肢頻繁撓頭（以連續(xù)5次以上撓頭為標準）行為出現(xiàn)時間點及消失（連續(xù)撓頭次數(shù)不及5次）時間點，并計算其持續(xù)時間；③分時間段統(tǒng)計大鼠撓頭次數(shù)：以30分鐘為一時間段，分時間段記錄自造模31分鐘后至180分鐘內(nèi)撓頭總次數(shù)。 5.采用酶聯(lián)免疫吸附法（ELISA）測定大鼠血清中SP、CGRP的含量。 結(jié)果： 1.模型對照組在皮下注射硝酸甘油后大概4min左右即出現(xiàn)耳紅、前肢頻繁撓頭、癥狀，此癥狀持續(xù)170-180分鐘不等。各組與同時間段空白對照組比較顯示在各個時間段內(nèi)均有前肢頻繁撓頭次數(shù)增多癥狀（P0.05）說明造模成功。 2.針刺對大鼠行為學變化的影響：對耳紅、前肢頻繁撓頭持續(xù)時間進行統(tǒng)計可發(fā)現(xiàn)針刺預防組、針刺治療組、針刺預防+治療組均能有效降低耳紅、前肢頻繁撓頭癥狀的持續(xù)時間，與模型對照組比較有統(tǒng)計學意義（P0.05）；以30min為一時間段，分時間段內(nèi)統(tǒng)計31-180min內(nèi)大鼠頻繁撓頭次數(shù)發(fā)現(xiàn)，針刺預防組、針刺治療組、針刺預防+治療組在各個時間段內(nèi)均能有效降低撓頭次數(shù)，與同時間段內(nèi)模型對照組比較P0.05，針刺預防+治療組與針刺預防組、針刺治療組比較療效更佳（P0.05）。 3.針刺對大鼠血清內(nèi)SP、CGRP含量變化的影響：針刺預防組與模型對照組比較：SP、CGRP含量降低，證明針刺對偏頭痛大鼠有預防作用（P0.05）；針刺治療組與模型對照組比較：SP、CGRP含量降低，證明針刺對偏頭痛大鼠有治療作用（P0.05）；針刺預防+治療組與針刺預防組、針刺治療組比較：SP、CGRP含量下降，證明針刺預防+治療組的療效優(yōu)于單純治療及單純預防（P0.05）。 結(jié)論： 1參照Cristina方法，成功復制出硝酸甘油型偏頭痛大鼠模型。 2.針刺可有效改善偏頭痛大鼠行為學變化，以針刺預防+治療組療效明顯。 3.硝酸甘油型偏頭痛大鼠模型血清內(nèi)SP、CGRP含量升高，推測偏頭痛的發(fā)生與SP介導的超敏反應與CGRP引起的血管過度擴張及神經(jīng)源性炎癥有關(guān)。 4.針刺可有效降低偏頭痛大鼠血清內(nèi)SP、CGRP含量，以針刺預防+治療組效果明顯。 5.針刺可能通過降低偏頭痛大鼠血清內(nèi)SP、CGRP的含量，進而緩解腦血管過度擴張及超敏反應，調(diào)節(jié)腦血流量，減輕神經(jīng)源性炎癥，來對偏頭痛產(chǎn)生預防及治療作用。
[Abstract]:Objective: To observe the effect of acupuncture on the behavioral changes of migraine rats and the effect of acupuncture on the changes of serum SP and CGRP contents, and to explore the mechanism of acupuncture prevention and treatment of migraine by replicating the model of migraine with subcutaneous injection of nitroglycerin.
Materials and methods:
1. of 50 healthy adult male SD rats, weighing about 250+50g, fed for one week after the start of the experiment. According to the random number table method, the rats were divided into 5 groups (blank control group, model control group, acupuncture prevention group, acupuncture group, acupuncture prevention + treatment group), 10 rats in each group. The blank control group: normal feeding for 7 days without any treatment; model control group: normal feeding for 7 days, eighth days subcutaneous injection of nitroglycerin model; acupuncture prevention group: acupuncture treatment for 7 days, 20min/d eighth days subcutaneous injection of nitroglycerin model; acupuncture treatment group: normal feeding for 7 days, eighth days subcutaneous injection Nitroglycerol., after the success of modeling, 20min acupuncture once; acupuncture prevention + treatment group: acupuncture treatment for 7 days, 20min/d eighth days, subcutaneous injection of Nitroglycerol., after the success of the model, acupuncture 20min once.
2. the rat model of migraine was prepared by subcutaneous injection of nitroglycerin (10mg/kg).
3. acupoints: Baihui, Yanglingquan (bilateral), Taichong (bilateral), Waiguan (bilateral).
4. to observe the behavior changes of rats after modeling, using the following three items as observation index: rat ear red duration: observe and record the rat red ears appear and disappear time points and calculate the duration; the rat forelimb duration: frequent scratching behavior were observed and recorded rat forelimb frequent scratching (for more than 5 consecutive head as the standard) appeared time and disappeared (continuous scratching number is less than 5 times) time point, and calculate the duration; the time statistics of rats: scratching frequency of 30 minutes for a period of time, the time period of record from the model after 31 minutes to 180 minutes. The total number of scratching.
5. the content of SP and CGRP in the serum of rats was determined by enzyme linked immunosorbent assay (ELISA).
Result:
The 1. model control group in the subcutaneous injection of nitroglycerin after about 4min appears red ear, forelimb frequent scratching, symptoms, the symptoms lasted 170-180 minutes. From the same time group and blank control group shows in different periods have increased the frequency of symptoms of forelimb frequent scratching (P0.05) indicated that the model was successful.
2. effect of acupuncture on rat behavior change: the red ear, frequent scratching duration of forelimb statistics can be found in acupuncture prevention group, acupuncture group, acupuncture prevention + treatment group can effectively reduce ear red, duration of symptoms and forelimb frequent scratching, compared with the model control group was statistically significant (P0.05) for a period of time; in 30min, divided time statistics 31-180min rats found frequent scratching frequency, acupuncture prevention group, acupuncture group, acupuncture prevention + treatment group can effectively reduce the scratching frequency in each time, with the same period in the model control group P0.05 prevention + treatment group and Acupuncture Acupuncture prevention group, acupuncture treatment group has better curative effect (P0.05).
3. acupuncture on rats serum SP, CGRP content effects: Acupuncture prevention group compared with the control group: SP, CGRP content decreased, that acupuncture has a preventive effect on migraine rats (P0.05); acupuncture treatment group compared with the control group: SP, CGRP content decreased, evidence of acupuncture therapy in migraine rats (P0.05); acupuncture prevention + treatment group and acupuncture prevention group, acupuncture treatment group comparison: SP, CGRP content decreased, showed that acupuncture efficacy in the prevention + treatment group was superior to that of simple treatment and Prevention (P0.05).
Conclusion:
1 the nitroglycerin type migraine rat model was successfully replicated with reference to the Cristina method.
2. acupuncture can effectively improve the behavioral changes of migraine rats, and the curative effect of acupuncture prevention + treatment group is obvious.
3. the nitroglycerin type migraine rat model increased serum SP and CGRP levels. It is speculated that the occurrence of migraine and SP mediated hypersensitivity are related to vascular overexpansion and neurogenic inflammation caused by CGRP.
4. acupuncture can effectively reduce the content of SP and CGRP in the serum of migraine rats, and the effect of acupuncture prevention + treatment group is obvious.
5. acupuncture can reduce the content of SP and CGRP in the serum of migraine rats, relieve cerebral vascular overexpansion and hypersensitivity reaction, regulate cerebral blood flow and reduce neurogenic inflammation, so as to prevent and treat migraine.

【學位授予單位】：遼寧中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R245

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