目的:研究黃芪多糖對(duì)A549/DDP肺腺癌細(xì)胞順鉑耐藥的影響及可能機(jī)制。方法:將A549/DDP順鉑耐藥肺腺癌細(xì)胞分為CON組（未行黃芪多糖及順鉑處理）、APS組（僅用黃芪多糖處理）、DDP組（僅用順鉑處理）及A+D組（黃芪多糖聯(lián)合順鉑處理）,比較四組細(xì)胞增殖、細(xì)胞周期及凋亡率和耐藥基因表達(dá)的差異。結(jié)果:A+D組細(xì)胞d₁-d₇吸光度、S期、G₂/M期和PI均顯著低于CON組、APS組和DDP組細(xì)胞（均P<0.05）,G₀/G₁期和凋亡率均顯著高于CON組、APS組和DDP組細(xì)胞（均P<0.05）;APS組和A+D組細(xì)胞MDR1和MRP基因mRNA表達(dá)無明顯差異（均P>0.05）,且均顯著低于CON組和DDP組（均P<0.05）。結(jié)論:黃芪多糖可顯著改善A549/DDP肺腺癌細(xì)胞對(duì)順鉑耐藥性,可能與黃芪多糖顯著降低MDR1和MRP有關(guān)。 

【文章來源】：現(xiàn)代腫瘤醫(yī)學(xué). 2020,28(13)

【文章頁數(shù)】：4 頁

【部分圖文】：

CON組、APS組、DDP組和A+D組細(xì)胞MDR1基因mRNA對(duì)比

CON組和DDP組A549/DDP順鉑耐藥肺腺癌細(xì)胞MDR1和MRP基因mRNA表達(dá)的差異均無統(tǒng)計(jì)學(xué)意義(均P>0.05);APS組和A+D組細(xì)胞MDR1和MRP基因mRNA表達(dá)的差異均無統(tǒng)計(jì)學(xué)意義(均P>0.05),且均顯著低于CON組和DDP組細(xì)胞(均P<0.05)(圖2和圖3)。表2 CON組、APS組、DDP組和A+D組細(xì)胞周期、增殖指數(shù)和凋亡率對(duì)比 (%, x ˉ ±s) Tab.2 Cell cycle,PI and apoptosis rate in group CON,group APS,group DDP and group A+D?(%, x ˉ ±s) Group G0/G1 phase S phase G2/M phase PI Apoptosis rate CON 53.1±2.1 31.2±1.3 6.9±0.9 40.6±1.6 9.8±1.4 APS 53.1±2.4 31.1±1.5 6.8±0.8 40.9±1.7 9.7±1.3 DDP 53.2±2.2 31.3±1.6 7.1±1.1 40.7±1.8 9.8±1.3 A+D 70.6±3.7* 20.5±1.2* 1.9±0.7* 23.5±1.2* 33.6±2.5* 注:A+D組vs CON組、APS組和DDP組,*表示P<0.05。Note:A+D group vs CON group,APS group and DDP group,*indicated P<0.05.

CON組、APS組、DDP組和A+D組細(xì)胞MRP基因mRNA對(duì)比

【參考文獻(xiàn)】：
期刊論文
[1]Astragalus polysaccharide attenuates rat experimental colitis by inducing regulatory T cells in intestinal Peyer’s patches[J]. Hai-mei Zhao,Yan Wang,Xiao-Ying Huang,min-fang Huang,Rong Xu,Hai-Yang Yue,Bu-gao Zhou,Hong-Yan Huang,Qi-meng Sun,Duan-Yong Liu.  World Journal of Gastroenterology. 2016(11)



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