【摘要】：目的探討體液中鈣離子濃度作為早期評(píng)價(jià)骨丟失間接指標(biāo)的可行性。方法采用尾懸吊模擬失重性骨丟失為動(dòng)物模型,懸吊組和對(duì)照組各8只3月齡SD雌性大鼠,懸吊時(shí)間為3周。大鼠每2 d收集1次尿液;每周麻醉1次稱重,并使用骨密度儀測(cè)量股骨和脛骨處的骨密度;實(shí)驗(yàn)結(jié)束大鼠麻醉后動(dòng)脈取血,分離血清,采用離子敏感電極技術(shù)檢測(cè)尿液和血清中鈣離子濃度。分離大鼠右側(cè)股骨和脛骨,烘烤后稱量骨干質(zhì)量和骨灰質(zhì)量。結(jié)果大鼠尾懸吊1周后體重明顯下降(P0.01);尿液中鈣離子濃度顯著升高(P0.01,P0.001);股骨的骨密度在第2周和第3周有明顯下降(P0.001);股骨骨干和骨灰質(zhì)量也有明顯變化(P0.05)。結(jié)論尿液中鈣離子濃度可以作為評(píng)價(jià)骨丟失的間接指標(biāo)。
[Abstract]:Objective to investigate the feasibility of calcium ion concentration in body fluid as an indirect index for early evaluation of bone loss. Methods tail suspension was used to simulate weightlessness bone loss in rats. There were 8 female SD rats aged 3 months in the suspension group and 8 female rats in the control group. The suspension time was 3 weeks. Urine was collected once every 2 days, weighed once a week under anesthesia, and bone mineral density (BMD) of femur and tibia was measured by BMD. At the end of the experiment, the blood was taken from the artery of the rats after anesthesia, and the serum was isolated, and the concentration of calcium in urine and serum was detected by ion sensitive electrode technique. The right femur and tibia were separated, and the bone mass and bone ash mass were measured after baking. Results after one week of tail suspension, the weight of rats decreased significantly (P0.01), the concentration of calcium in urine increased significantly (P0.01 P0.001), the bone mineral density of femur decreased significantly at week 2 and week 3 (P0.001). The quality of femur diaphysis and ashes also changed significantly (P0.05). Conclusion the calcium concentration in urine can be used as an indirect index to evaluate bone loss.
【作者單位】： 西北工業(yè)大學(xué)生命學(xué)院特殊環(huán)境生物物理學(xué)研究所空間生物模擬技術(shù)國(guó)防重點(diǎn)學(xué)科實(shí)驗(yàn)室;中國(guó)航天員科研訓(xùn)練中心航天醫(yī)學(xué)基礎(chǔ)與應(yīng)用國(guó)家重點(diǎn)實(shí)驗(yàn)室;
【基金】：載人航天領(lǐng)域第一批預(yù)先研究項(xiàng)目(010201) 西北工業(yè)大學(xué)研究生創(chuàng)業(yè)種子基金(Z2010089)資助
【分類號(hào)】：R852.22
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本文編號(hào)：2296393