發(fā)布時間：2018-08-26 20:06

【摘要】：目的　篩選大鼠經(jīng)正加速度 (Gositiveacceleration ,+Gz)重復(fù)暴露后腦的差異表達(dá)基因 ,探討 +Gz重復(fù)暴露致腦損傷的分子機(jī)制。方法　應(yīng)用抑制性消減雜交技術(shù) ,構(gòu)建高消減效率的 +Gz重復(fù)暴露大鼠腦cDNA消減文庫 ;用差異篩選技術(shù)篩選陽性克隆 ;用序列分析確定陽性克隆的性質(zhì) ;用RT -PCR證實(shí)陽性克隆的可靠性。結(jié)果　從消減庫中獲得70個陽性克隆 ;經(jīng)差異篩選后 ,選取其中 10個陽性克隆 ,序列分析表明 ,7個克隆與已知基因高度同源 ,3個為新的候選基因。RT -PCR證實(shí)了差異表達(dá)基因在 +Gz重復(fù)暴露大鼠腦中高表達(dá)。結(jié)論　用抑制性消減雜交技術(shù)篩選發(fā)現(xiàn)的 3個新的cDNA可能在 +Gz腦損傷的病理過程中起重要作用
[Abstract]:Objective to screen differentially expressed genes in rat brain after repeated exposure to positive acceleration (Gositiveacceleration, Gz), and to explore the molecular mechanism of brain injury induced by repeated Gz exposure. Methods the suppression subtractive hybridization technique was used to construct the cDNA subtractive library with high subtractive efficiency for repeated exposure of Gz in rat brain, the positive clones were screened by differential screening technique, the properties of the positive clones were determined by sequence analysis. The reliability of positive clones was confirmed by RT-PCR. Results 70 positive clones were obtained from subtractive library and 10 of them were selected after differential screening. Sequence analysis showed that 7 clones were highly homologous to the known genes, and 3 were new candidate genes. RT-PCR confirmed the overexpression of differentially expressed genes in the brain of rats exposed to repeated Gz exposure. Conclusion three new cDNA detected by suppression subtractive hybridization may play an important role in the pathological process of Gz brain injury.
【作者單位】： 第三軍醫(yī)大學(xué)附屬西南醫(yī)院病理學(xué)研究所 空軍總醫(yī)院臨床分子生物學(xué)研究中心 第三軍醫(yī)大學(xué)附屬西南醫(yī)院病理學(xué)研究所 航空病研究中心 空軍總醫(yī)院臨床分子生物學(xué)研究中心
【基金】：空軍后勤部科研基金資助項(xiàng)目 (KH99190 76 )
【分類號】：R82

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 孫喜慶;反復(fù)高G值暴露對腦的潛在影響及其機(jī)制[J];航天醫(yī)學(xué)與醫(yī)學(xué)工程;1998年01期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 郝唯蔚,俞啟福,趙云濤;飛行因素對人體血漿甘丙素的影響及其意義[J];海軍總醫(yī)院學(xué)報;2000年02期

2 蔡慶,劉紅巾,占志,朱美財;+Gz重復(fù)暴露大鼠海馬細(xì)胞凋亡及相關(guān)基因bcl-2和p53表達(dá)的觀察[J];航天醫(yī)學(xué)與醫(yī)學(xué)工程;2000年04期

3 劉紅巾,蔡慶,紀(jì)桂英,占志,姜建東,朱美財;+Gz重復(fù)暴露大鼠腦組織中IL-1β和TNF-αmRNA表達(dá)變化[J];航天醫(yī)學(xué)與醫(yī)學(xué)工程;2000年05期

4 劉紅巾,蔡慶,紀(jì)桂英,王U，

本文編號：2206016