本文選題：海馬 + 神經(jīng)元　； 參考：《第四軍醫(yī)大學(xué)學(xué)報》2004年02期

【摘要】：目的 :采用動物離心機建立模擬推拉動作的動物模型 ,探討“推拉效應(yīng)”對大鼠海馬神經(jīng)元bFGF表達的影響 .方法 :健康雄性Wistar大鼠 4 0只 ,隨機分為 1 0組 (對照組 ,三種強度暴露后的 30min,6h ,2 4h組 ) ,每組 4只 ,灌注固定 ,取大腦半球 ,切片后應(yīng)用免疫組織化學(xué)染色及圖像分析技術(shù)對大鼠海馬各區(qū)神經(jīng)元堿性成纖維細(xì)胞生長因子 (bFGF)表達進行檢測 .結(jié)果 :對照組和± 2Gz暴露后的 30min組bFGF呈弱陽性表達 ;而± 6Gz和± 8Gz暴露后 30min的實驗組可見大鼠海馬神經(jīng)元細(xì)胞bFGF表達開始增加 ,6h實驗組表達明顯增強 ,2 4h后表達下降但仍較正常對照組高 .與對照組比較 ,差異有顯著性 (P 0 .0 5 ) .結(jié)論 :中、高強度的推拉動作可引起大鼠海馬神經(jīng)元bFGF表達增加
[Abstract]:Aim: to establish an animal model with animal centrifuge to simulate push-pull action and to investigate the effect of "push-pull effect" on the expression of bFGF in hippocampal neurons of rats. Methods: forty healthy male Wistar rats were randomly divided into 10 groups (control group, 30 min after exposure to three intensities for 6 h and 24 h), each group (4 rats) were infused and fixed, and the hemispheres were taken. The expression of basic fibroblast growth factor (bFGF) in hippocampal neurons of rats was detected by immunohistochemical staining and image analysis. Results: the expression of bFGF was weakly positive in control group and 鹵2Gz exposed 30min group. The expression of bFGF in hippocampal neurons of rats exposed to 鹵6Gz and 鹵8Gz showed that the expression of bFGF began to increase at 6 h after exposure, and the expression of bFGF in experimental group decreased significantly after 24 h of exposure, but it was still higher than that in normal control group. Compared with the control group, the difference was significant (P 0. 05). Conclusion: the expression of bFGF in rat hippocampal neurons can be increased by high-intensity push-pull exercise.
【作者單位】： 第四軍醫(yī)大學(xué)吉林軍醫(yī)學(xué)院醫(yī)學(xué)實驗技術(shù)教研室 第四軍醫(yī)大學(xué)吉林軍醫(yī)學(xué)院醫(yī)學(xué)實驗技術(shù)教研室 第四軍醫(yī)大學(xué)吉林軍醫(yī)學(xué)院醫(yī)學(xué)實驗技術(shù)教研室 第四軍醫(yī)大學(xué)吉林軍醫(yī)學(xué)院醫(yī)學(xué)實驗技術(shù)教研室 第四軍醫(yī)大學(xué)吉林軍醫(yī)學(xué)院醫(yī)學(xué)實驗技術(shù)教研室 第四軍醫(yī)大學(xué)吉林軍醫(yī)學(xué)院醫(yī)學(xué)實驗技術(shù)教研室
【基金】：全軍醫(yī)藥科研基金項目 (0 1MA2 0 2 )
【分類號】：R852

【參考文獻】

相關(guān)期刊論文 前3條

1 張五星,詹長錄,耿喜臣;在加速度作用中的推拉效應(yīng)[J];中華航空航天醫(yī)學(xué)雜志;1999年01期

2 李華軍,李巍,陳東,田沈;全腦缺血再灌注后遲發(fā)性神經(jīng)元壞死與細(xì)胞因子bFGF的相關(guān)性研究[J];中國老年學(xué)雜志;2002年03期

3 沈明韶,任明,邵福源;實驗性腦缺血及再灌注大鼠腦組織bFGF表達的研究[J];中國神經(jīng)免疫學(xué)和神經(jīng)病學(xué)雜志;1999年03期

【共引文獻】

相關(guān)期刊論文 前10條

1 張素,陳崇宏,鄭志，

本文編號：2092366