發(fā)布時間：2018-04-18 04:39

  本文選題：Ca + 中藥��； 參考：《中國中藥雜志》2009年09期

【摘要】：目的:研究中藥復方對模擬失重大鼠承重骨對外源鈣攝取的干預作用。方法:雄性Wistar大鼠隨機分為3組,空白組、模型組、中藥組。尾吊模擬失重,中藥復方(含熟地黃、懷牛膝、黃芪、當歸、牡蠣醋酸水解物等)704 g.L-1水煎劑2.5mL灌胃;適應期1周,模擬失重期3周,于模擬失重第11天一次性灌胃給予一定量41Ca示蹤劑。實驗結束取股骨,加速器質譜法(Accelerator Mass Spectrometry,AMS)分析骨41Ca/40Ca值,電感耦合等離子體光譜法(Inductively Coupled Plasma-AtomicEmission Spectroscopy,ICP-AES)分析骨鈣總量,計算股骨41Ca沉積量、股骨41Ca沉積率。結果:空白、模型、中藥3組股骨41Ca/40Ca豐度比分別為(5.03±0.55),(3.82±0.29),(5.73±0.40)×10-9,模型組較空白組顯著降低(P0.001),中藥組較空白組顯著升高(P0.05);空白、模型、中藥3組股骨41Ca沉積量分別為(7.39±0.90),(4.52±0.40),(7.45±0.89)×10-7mg,沉積率分別為(0.819±0.100),(0.501±0.045),(0.826±0.098)%,模型組股骨41Ca沉積量和沉積率均較空白組顯著降低(P0.001),中藥組較空白組變化不顯著,較模型組顯著增高(P0.001)。結論:尾吊模擬失重可造成大鼠承重骨外源鈣沉積障礙,中藥可較好對抗這種情況;失重骨質疏松可能存在外源鈣沉積減少以外的多種機制。
[Abstract]:Aim: to study the effect of Chinese medicine compound on exogenous calcium uptake in weight-bearing bone of simulated weightlessness rats.Methods: male Wistar rats were randomly divided into 3 groups: blank group, model group and traditional Chinese medicine group.Tail suspension simulated weightlessness, traditional Chinese medicine compound (including Radix Rehmanniae, Achyranthes bidentata, Radix Astragali, Angelica sinensis, oyster acetic acid hydrolysate, etc.) 2.5mL, the adaptation period was 1 week, and the simulated weightlessness period was 3 weeks.A certain amount of 41Ca tracer was given once on the 11th day of simulated weightlessness.At the end of the experiment, the femur was analyzed by Accelerator Mass Spectrometry (AMS), and the total bone calcium was analyzed by inductively Coupled Plasma-AtomicEmission spectroscopy (ICP-AESS-MS). The deposition of 41Ca in femur and 41Ca deposition rate in femur were calculated.Results: the 41Ca/40Ca abundance ratio of femur in the blank, model and TCM groups was 5.03 鹵0.55, 3.82 鹵0.29, 5.73 鹵0.40) 脳 10 ~ (-9), respectively. Compared with the blank group, the 41Ca/40Ca abundance ratio of the model group was significantly lower than that of the blank group, and that of the traditional Chinese medicine group was significantly higher than that of the blank group, while the blank, the model group was significantly higher than that of the blank group.The deposition amount of 41Ca in the femur of the three groups were 7.39 鹵0.90, 4.52 鹵0.40, 7.45 鹵0.89) 脳 10 ~ (-7) mg, and the deposition rates were 0.819 鹵0.100 鹵0.501 鹵0.045, 0.826 鹵0.098, respectively. The 41Ca deposition and deposition rate of the femur in the model group were significantly lower than those in the blank group (P 0.001), but the changes in the traditional Chinese medicine group were not significant compared with the blank group, and significantly higher than that in the model group.Conclusion: tail suspension simulated weightlessness can cause exogenous calcium deposition obstacle in load-bearing bone of rats, Chinese medicine can better resist this situation, and weightlessness osteoporosis may have some mechanisms other than the reduction of exogenous calcium deposition.
【作者單位】： 北京中醫(yī)藥大學基礎醫(yī)學院;天津中醫(yī)藥大學中藥學院;中國原子能科學研究院;
【基金】：國家自然科學基金項目(30500663) 2006年教育部優(yōu)秀人才支持計劃項目(NCET-06-0124) 2006年北京市科技新星(A)類計劃項目(2006A49) 國家基礎科學人才培養(yǎng)基金(20070110)
【分類號】：R85

【二級參考文獻】

相關期刊論文 前2條

1 范全春,李勇枝,白桂娥,段淵儒,謝俊水,石宏志;強骨抗萎方對模擬失重大鼠骨代謝影響的觀察[J];航天醫(yī)學與醫(yī)學工程;2003年02期

2 黃紀明,朱德兵,李志霞,李培榮,洪建武,白樹民;營養(yǎng)素合劑對模擬失重大鼠股骨的影響[J];中國骨質疏松雜志;2005年01期

【相似文獻】

相關會議論文 前1條

1 傅騫;胡素敏;周鵬;楊佳佳;李瑾;佟海英;;尾部懸吊3周及中藥干預對模擬失重大鼠全身骨密度的影響[A];中國空間科學學會第七次學術年會會議手冊及文集[C];2009年

，

本文編號：1766862