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  本文選題：氧驚厥　切入點：原花青素　出處：《第二軍醫(yī)大學》2005年碩士論文　論文類型：學位論文

【摘要】：急性氧中毒是當機體暴露在氧壓大于200 kPa(2 ATA)的環(huán)境中時,可能發(fā)生的一種疾病,主要表現(xiàn)為中樞神經(jīng)系統(tǒng)的癥狀,類似于癲癇大發(fā)作。近年來,隨著高壓氧治療在臨床上日益廣泛的應用,以及人們在進軍海洋過程中潛水活動逐漸增多,接觸高壓氧的機會也越來越多。由于人體的氧耐受性、儀器設備、操作方法等因素,發(fā)生急性氧中毒的可能性有所增加,所以做好急性氧中毒的預防工作十分必要。藥物預防是一種必要的輔助方式。雖然也對一些藥物進了研究,但是由于某些藥物在預防效果上存在問題,或者是其在使用過程中所帶來的毒副作用令人困擾,因此到目前為止,尚沒有找到較好的預防藥物。 我們的實驗對原花青素(procyanidins,PC)的預防作用進行了觀察。結(jié)合急性氧中毒致病機理的研究,提出了PC的預防作用主要是與它的抗氧化特性和對腦血管的保護作用有關的假設。圍繞這一假設,我們觀察了PC對高壓氧暴露導致小鼠驚厥潛伏期、驚厥大發(fā)作時間和脫離高壓氧環(huán)境恢復期的影響,為了研究其具體的預防機制,我們同時測定了接受高壓氧暴露后的小鼠腦組織中活性氧(reactive oxygen species,ROS)、丙二醛(malonaldehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)等與機體氧化-抗氧化系統(tǒng)有關指標的含量變化以及腦內(nèi)重要的抑制性神經(jīng)遞質(zhì)γ-氨基丁酸(γ-GABA)的含量變化。 研究結(jié)果顯示,PC可以明顯延長5個大氣壓的純氧暴露下小鼠氧驚厥的潛伏期,同時可明顯減少經(jīng)上述處理的小鼠皮層和海馬中ROS、MDA的含量,增加SOD的含量,以及增加腦組織中GABA的含量。 通過研究我們認為,PC具有肯定的預防氧驚厥的作用,這種預防作用可能與以下兩個因素有關:一是它有較強的氧自由基清除活性,從而增強機體的抗氧化能力;二是通過抗氧化作用,間接調(diào)節(jié)腦內(nèi)的神經(jīng)遞質(zhì)的平衡及穩(wěn)定腦組織內(nèi)環(huán)境等作用對腦組織產(chǎn)生保護作用。
[Abstract]:Acute oxygen poisoning is a disease that may occur when the body is exposed to an oxygen pressure greater than 200 kPa(2 ATAs, mainly manifested as symptoms of the central nervous system, similar to seizures in recent years. With the increasing clinical application of hyperbaric oxygen therapy, and the increasing number of diving activities in the process of entering the ocean, the chances of exposure to hyperbaric oxygen are also increasing. The possibility of acute oxygen poisoning has increased, so it is necessary to do a good job in the prevention of acute oxygen poisoning. Drug prevention is a necessary auxiliary method. Although some drugs have also been studied, However, because some drugs have some problems in the preventive effect, or because of the harmful side effects in the course of their use, so far, no good preventive drugs have been found. The preventive effect of procyanidins PCS was observed in our experiment. The prophylaxis of PC is mainly related to its antioxidant properties and its protective effect on cerebral vessels. Around this hypothesis, we observed the latent period of convulsion induced by hyperbaric oxygen exposure in mice. The effect of convulsion time and recovery from hyperbaric oxygen environment, in order to study its specific prevention mechanism, We also measured the contents of reactive oxygen species (oxygen), malondialdehyde (MDA) and superoxide dismutase (SOD) in the brain tissues of mice exposed to hyperbaric oxygen (HBO), and the changes of the contents of the indexes related to the oxidation-antioxidant system and the important inhibition in the brain. Changes of 緯-aminobutyric acid (緯 -GABA), a neurotransmitter. The results showed that PC could significantly prolong the incubation period of oxygen convulsion in mice exposed to pure oxygen at 5 atmospheres, at the same time, it could significantly reduce the content of SOD in cortex and hippocampus of mice treated above, and increase the content of SOD. And increase the content of GABA in brain tissue. Through the study, we think that PC can prevent oxygen convulsion, this preventive effect may be related to the following two factors: first, it has strong oxygen free radical scavenging activity, thereby enhancing the antioxidant capacity of the body; The other is to indirectly regulate the balance of neurotransmitters in brain and stabilize the environment of brain tissue through antioxidation.
【學位授予單位】：第二軍醫(yī)大學
【學位級別】：碩士
【學位授予年份】：2005
【分類號】：R82

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相關期刊論文 前1條

1 李峻,肖衛(wèi)兵,張慶陵,任利蕙;連續(xù)腦電圖振幅譜在高氣壓環(huán)境下應用的方法探討[J];中華航海醫(yī)學與高氣壓醫(yī)學雜志;2003年04期

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本文編號：1650361