本文關(guān)鍵詞： 海水浸泡 疼痛 超微結(jié)構(gòu) 自噬　出處：《解放軍醫(yī)學(xué)雜志》2016年07期 　論文類型：期刊論文

【摘要】：目的評價局部海水浸泡對皮膚/肌肉切口牽拉痛(SMIR)模型大鼠疼痛行為學(xué)及繼發(fā)神經(jīng)病理改變的影響。方法選擇健康成年雄性SD大鼠48只,隨機(jī)分為模型對照組(MC組,只建立SMIR模型,不予浸泡處理)、生理鹽水浸泡組(NS組,建立模型后局部傷口給予生理鹽水浸泡1h)、海水浸泡組(SW組,建立模型后局部傷口給予海水浸泡1h),于術(shù)前1d及術(shù)后1、3、5、7、14、21、28d測定機(jī)械縮足反射閾值(MWT),于術(shù)后3、28d各組分別隨機(jī)取8只大鼠,取術(shù)側(cè)隱神經(jīng)行HE染色、取術(shù)側(cè)L3及L4背根神經(jīng)節(jié)應(yīng)用電鏡技術(shù)觀察其超微結(jié)構(gòu)變化。結(jié)果 SW組術(shù)后各時點MWT值均明顯低于NS組和MC組,且至術(shù)后28d仍明顯低于術(shù)前基礎(chǔ)水平;NT組和NS組術(shù)后各時點MWT值無明顯差異,兩組術(shù)后28d的MWT值與術(shù)前基礎(chǔ)水平相當(dāng)。SW組術(shù)后3、28d隱神經(jīng)HE染色顯示均有不同程度的神經(jīng)損傷表現(xiàn),而MC組和NS組未見異常;術(shù)后3、28d電鏡觀察見SW組背根神經(jīng)節(jié)神經(jīng)元內(nèi)有大量空泡樣線粒體和自噬小體,MC組和NS組未見明顯病理改變。結(jié)論海水浸泡局部傷口可加重SMIR模型大鼠的急性疼痛,并可能導(dǎo)致其疼痛向慢性化轉(zhuǎn)變,海水浸泡導(dǎo)致的外周神經(jīng)損傷、背根神經(jīng)節(jié)神經(jīng)元超微結(jié)構(gòu)改變可能參與了其疼痛行為學(xué)改變的機(jī)制。
[Abstract]:Objective to evaluate the effects of local seawater immersion on pain behavior and secondary neuropathological changes in skin / muscle incision stretch pain rats. Methods 48 healthy adult male SD rats were randomly divided into model control group and control group. The SMIR model was established only. The saline immersion group was treated with NS group. After the model was established, the local wound was given saline immersion for 1 hour, and the seawater immersion group was given the SW group. After the model was established, the local wound was treated with seawater immersion for 1 hour. The mechanical foot reflex threshold (MWTT) was measured at 1 day before operation and 1 day after operation. Eight rats were randomly selected from each group on the 3rd and 28th days after operation. The saphenous nerve of the operative side was stained with HE. The ultrastructural changes of L3 and L4 dorsal root ganglion were observed by electron microscopy. Results the MWT values in SW group were significantly lower than those in NS group and MC group at all time points after operation. At 28 days after operation, the MWT values of NT group and NS group were still significantly lower than those of preoperative basic level NT group and NS group, and there was no significant difference at each time point between NT group and NS group. The MWT values of the two groups on the 28th day after operation were similar to the basic level before operation. The HE staining of the saphenous nerve in the SW group showed different degrees of nerve injury on the 38th day after operation, but there was no abnormality in the MC group and NS group. A large number of vacuolated mitochondria and autophagy bodies in the neurons of dorsal root ganglion neurons in group SW were observed by electron microscope on the 328th day after operation. There were no obvious pathological changes in MC group and NS group. Conclusion the acute pain of SMIR model rats can be aggravated by seawater immersion in local wound. It may lead to the transformation of pain to chronic, the peripheral nerve injury caused by seawater immersion, and the ultrastructural changes of dorsal root ganglion neurons may be involved in the mechanism of pain behavior changes.
【作者單位】： 廣州軍區(qū)廣州總醫(yī)院麻醉科全軍臨床麻醉中心;
【基金】：全軍醫(yī)學(xué)科研“十二五”科技攻關(guān)項目(BWS11J016)~~
【分類號】：R82

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 王海峰;丁宏霞;胡灃;洪鶴;李葛巍;符來想;方健;;海水浸泡對大鼠坐骨神經(jīng)損傷后促炎因子表達(dá)的影響及早期干預(yù)效果觀察[J];解放軍醫(yī)學(xué)雜志;2015年10期

2 寧浩勇;孟宇宏;劉肖;王大鵬;虞積耀;;火器傷合并海水浸泡后的愈合過程觀察[J];中國醫(yī)藥導(dǎo)刊;2015年01期

3 浦江;崔立紅;劉超群;吳姍珊;李欣;李輝;羅哲;;黃體酮對海水浸泡腹部開放傷大鼠胃黏膜損傷的保護(hù)作用[J];解放軍醫(yī)學(xué)雜志;2013年01期

【共引文獻(xiàn)】

相關(guān)期刊論文 前5條

1 曹國麗;劉倩倩;王敏;易廷莊;湯紹輝;;適量飲酒與脂肪性肝病或非酒精性脂肪性肝病發(fā)病風(fēng)險的Meta分析[J];解放軍醫(yī)學(xué)雜志;2016年07期

2 鄧榮芳;黃希照;溫雪飛;屠偉峰;;局部海水浸泡對SMIR模型大鼠疼痛行為學(xué)和繼發(fā)神經(jīng)病理改變的影響[J];解放軍醫(yī)學(xué)雜志;2016年07期

3 王代勇;徐先鋒;唐玉娟;王瑜;王烈;張再重;;瀕海戰(zhàn)創(chuàng)傷動物模型腹腔感染細(xì)菌學(xué)特點及藥敏試驗研究[J];中華災(zāi)害救援醫(yī)學(xué);2015年10期

4 Hai Jin;Li-Jun Hou;Xiao-Bing Fu;;Medical rescue of naval combat: challenge and future[J];Military Medical Research;2015年02期

5 高娟;鄭國榮;王艷;李e，

本文編號：1518504