CsA干預(yù)下長(zhǎng)時(shí)間耐力運(yùn)動(dòng)對(duì)大鼠Caveolin-3及相關(guān)指標(biāo)的影響
發(fā)布時(shí)間：2019-06-26 09:18
【摘要】：研究目的：在CsA干預(yù)條件下，長(zhǎng)時(shí)間大強(qiáng)度的耐力運(yùn)動(dòng)對(duì)細(xì)胞膜caveolin-3含量的影響情況。結(jié)合一些生化指標(biāo)和細(xì)胞內(nèi)離子濃度的變化，探討caveolin-3在耐力運(yùn)動(dòng)中參與對(duì)運(yùn)動(dòng)引起細(xì)胞蛋白代謝、對(duì)細(xì)胞信號(hào)通路的影響及caveolin-3自身變化機(jī)制，為耐力運(yùn)動(dòng)造成的細(xì)胞結(jié)構(gòu)的變化以及所引起的疲勞快速恢復(fù)提供理論依據(jù)。 研究方法：研究對(duì)象選用6周齡大的SD雄性大鼠40只，隨機(jī)分成安靜組（C）、耐力訓(xùn)練組（T）、環(huán)孢霉素組（S）和環(huán)孢霉素訓(xùn)練組（ST），每組10只。適應(yīng)性訓(xùn)練兩周后，以28m/s，10°坡度，每天訓(xùn)練1小時(shí)，每周休息1天的強(qiáng)度訓(xùn)練13周，11-13周ST組和S組注射CsA。腹主動(dòng)脈取血，取腓腸肌、比目魚肌、趾長(zhǎng)伸肌。免疫組化法測(cè)Caveolin-3含量；熒光指示法測(cè)定Ca~(2+)；采用速率法測(cè)定血液中CK、LDH及組織LDH的含量；硝酸還原酶法測(cè)定組織中NO、NOS的含量。 實(shí)驗(yàn)結(jié)果： （1）長(zhǎng)時(shí)間大強(qiáng)度運(yùn)動(dòng)后，環(huán)孢霉素組（S）大鼠細(xì)胞內(nèi)Ca~(2+)濃度與安靜組（C）相比無顯著性差異（p0.05）；耐力訓(xùn)練組（T）極其顯著高于安靜組（C）（p0.01）；環(huán)孢霉素耐力訓(xùn)練組（ST）顯著高于安靜組（C）（p0.05）。 （2）NO測(cè)試結(jié)果為：環(huán)孢霉素組（S）顯著高于安靜組（C）（p0.05）；耐力訓(xùn)練組（T）與安靜對(duì)照組（C）相比，無顯著性差異（p0.05）；環(huán)孢霉素耐力訓(xùn)練組（ST）極其顯著高于安靜組（C）（p0.01），顯著高于運(yùn)動(dòng)對(duì)照組（p0.05）。 （3）總NOS測(cè)得結(jié)果為：環(huán)孢霉素組（S）與安靜組（C）相比，TNOS含量極其顯著增高（p0.01）；耐力訓(xùn)練組（T）與安靜組（C）相比，TONS含量顯著升高（p0.05）；環(huán)孢耐力訓(xùn)練組（ST）與安靜組（C）相比，無顯著性差異（p0.05）。 （4）CK測(cè)試結(jié)果：各組間無顯著性差異（p0.05）。 （5）血液中LDH測(cè)試結(jié)果為環(huán)孢霉素組顯著高于安靜組（p0.05）；其它組間相比無顯著性差異（p0.05）。組織中測(cè)試結(jié)果為：各組間無顯著性差異（p0.05）。 （6）Caveolin-3的測(cè)試結(jié)果顯示：趾長(zhǎng)伸肌中的含量由高到低的順序?yàn)镃組、ST組、T組、S組；比目魚肌中的含量由高到低順序?yàn)镃組、T組、S組、ST組。 實(shí)驗(yàn)結(jié)論： 1.環(huán)孢霉素A會(huì)造成骨骼肌細(xì)胞膜的損傷。 2.長(zhǎng)時(shí)間耐力運(yùn)動(dòng)后細(xì)胞內(nèi)Ca~(2+)濃度上升，單獨(dú)CsA干預(yù)對(duì)胞漿Ca~(2+)濃度變化影響不大。耐力運(yùn)動(dòng)與CsA共同作用下，，細(xì)胞內(nèi)Ca~(2+)濃度上升。耐力運(yùn)動(dòng)干預(yù)條件對(duì)胞漿內(nèi)Ca~(2+)濃度的影響明顯高于CsA干預(yù)條件。 3.CsA干預(yù)條件下，骨骼肌組織中NOS與NO變化一致，都會(huì)升高。耐力訓(xùn)練下，肌組織中NOS會(huì)升高，NO的生成受到影響。CsA與耐力運(yùn)動(dòng)共同影響下，NOS變化不明顯，NO含量增加。 4.CsA干預(yù)下，組織中LDH含量上升，CK變化恢復(fù)到安靜時(shí)水平。與CK相比，LDH恢復(fù)的更慢，反映損傷的時(shí)間更長(zhǎng)。 5.長(zhǎng)時(shí)間耐力運(yùn)動(dòng)、CsA干預(yù)與二者協(xié)同作用都能夠造成不同纖維類型肌肉中Caveolin-3含量的降低，影響Caveolin-3介導(dǎo)的信號(hào)通路。CsA對(duì)比目魚肌為代表的慢肌纖維的影響較以趾長(zhǎng)伸肌為代表的快肌纖維的影響更為顯著。
[Abstract]:Objective: To study the effect of long-time and long-time endurance exercise on the content of caveolin-3 in cell membrane under the condition of CsA intervention. In combination with that change of the biochemical index and the ion concentration in the cell, the influence of cave1-3 on the cellular protein metabolism, the effect on the cell signal path and the mechanism of the self-change of the cave1-3 in the endurance exercise is discussed. And provides a theoretical basis for the change of the cell structure caused by the endurance exercise and the induced fatigue rapid recovery. Methods:40 SD male rats,6 weeks old, were randomly divided into a quiet group (C), a endurance training group (T), a cyclosporine group (S) and a cyclosporine training group (ST), each group 10 1-hour training at 28 m/ s,10 擄 grade,1 hour daily,1 day off-week,13-week,11-13-week ST and S-group injections A. Take blood from the abdominal aorta, and take the gastrocnemius muscle, the flounder muscle and the toe The content of serum CK, LDH and LDH in blood was determined by the method of rate, and the content of NO and NOS in the tissues was determined by the method of nitric acid reductase. Quantity. Real Results: (1) The intracellular Ca ~ (2 +) concentration in the cyclosporine group (S) was significantly higher than that of the quiet group (C) after long-time high-intensity exercise (p0.05); the endurance training group (T) was significantly higher than that of the quiet group (C) (p 0.01); the cyclosporine endurance training group (ST) was significantly higher than that of the quiet group (C) (p (2) The result of NO test was that the group of cyclosporine (S) was significantly higher than that of the quiet group (C) (p0.05); the endurance training group (T) was significantly higher than that of the quiet group (C) (p0.05); the cyclosporine endurance training group (ST) was significantly higher than that of the quiet group (C). (p0.01), significantly higher than the control group (p0.05). (3) The total NOS was found to be significantly higher than that of the quiet group (C) (p0.01), and the content of TONS was significantly higher in the endurance training group (T) than in the quiet group (C). (p0.05); There was no significant difference in the endurance training group (ST) and the quiet group (C). Difference (p0.05). (4) CK test results: no significant difference between groups (5) The results of LDH in blood were significantly higher in the group than in the quiet group (p0.05); the other groups were compared with other groups. There was no significant difference (p0.05). The results of the test in the tissue were: There was no significant difference (p0.05). (6) The test results of CaveI-3 showed that the content in the extensor digitorum longus was from high to low in group C, ST group, T group, S group, and the content of soleus muscle was from high to low. The order is Group C T, S and ST. 1. Cyclosporin A may cause damage to the cell membrane of the skeletal muscle.2. The concentration of Ca ~ (2 +) in the cells increased after the long-time endurance exercise. The effect of A-intervention on the concentration of Ca ~ (2 +) in the cytoplasm is not significant. The concentration of Ca ~ (2 +) in the cells increased with the effect of A. The effect of concentration of ~ (2 +) was significantly higher than that of CsA. The changes of NOS and NO in skeletal muscle tissue will increase. Endurance training In practice, the NOS in the muscle tissue will increase and the production of NO is affected. CsA and endurance Under the influence of exercise, the change of NOS was not obvious and the content of NO increased.4. CsA intervention in that next group, the LDH content in the tissue increase and the CK change is restored to a quiet time . LDH recovery is slower than CK, and the time to reflect the damage is longer.5. Long-term endurance exercise, CsA intervention and the synergistic effect of both can cause Caveolin in the muscle of different fiber types. the reduction of the content of -3, affecting the signaling pathway mediated by the Caveolin-3. CsA is a slow representative of the soleus muscle
【學(xué)位授予單位】：遼寧師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：G804.2
本文編號(hào)：2506085
[Abstract]:Objective: To study the effect of long-time and long-time endurance exercise on the content of caveolin-3 in cell membrane under the condition of CsA intervention. In combination with that change of the biochemical index and the ion concentration in the cell, the influence of cave1-3 on the cellular protein metabolism, the effect on the cell signal path and the mechanism of the self-change of the cave1-3 in the endurance exercise is discussed. And provides a theoretical basis for the change of the cell structure caused by the endurance exercise and the induced fatigue rapid recovery. Methods:40 SD male rats,6 weeks old, were randomly divided into a quiet group (C), a endurance training group (T), a cyclosporine group (S) and a cyclosporine training group (ST), each group 10 1-hour training at 28 m/ s,10 擄 grade,1 hour daily,1 day off-week,13-week,11-13-week ST and S-group injections A. Take blood from the abdominal aorta, and take the gastrocnemius muscle, the flounder muscle and the toe The content of serum CK, LDH and LDH in blood was determined by the method of rate, and the content of NO and NOS in the tissues was determined by the method of nitric acid reductase. Quantity. Real Results: (1) The intracellular Ca ~ (2 +) concentration in the cyclosporine group (S) was significantly higher than that of the quiet group (C) after long-time high-intensity exercise (p0.05); the endurance training group (T) was significantly higher than that of the quiet group (C) (p 0.01); the cyclosporine endurance training group (ST) was significantly higher than that of the quiet group (C) (p (2) The result of NO test was that the group of cyclosporine (S) was significantly higher than that of the quiet group (C) (p0.05); the endurance training group (T) was significantly higher than that of the quiet group (C) (p0.05); the cyclosporine endurance training group (ST) was significantly higher than that of the quiet group (C). (p0.01), significantly higher than the control group (p0.05). (3) The total NOS was found to be significantly higher than that of the quiet group (C) (p0.01), and the content of TONS was significantly higher in the endurance training group (T) than in the quiet group (C). (p0.05); There was no significant difference in the endurance training group (ST) and the quiet group (C). Difference (p0.05). (4) CK test results: no significant difference between groups (5) The results of LDH in blood were significantly higher in the group than in the quiet group (p0.05); the other groups were compared with other groups. There was no significant difference (p0.05). The results of the test in the tissue were: There was no significant difference (p0.05). (6) The test results of CaveI-3 showed that the content in the extensor digitorum longus was from high to low in group C, ST group, T group, S group, and the content of soleus muscle was from high to low. The order is Group C T, S and ST. 1. Cyclosporin A may cause damage to the cell membrane of the skeletal muscle.2. The concentration of Ca ~ (2 +) in the cells increased after the long-time endurance exercise. The effect of A-intervention on the concentration of Ca ~ (2 +) in the cytoplasm is not significant. The concentration of Ca ~ (2 +) in the cells increased with the effect of A. The effect of concentration of ~ (2 +) was significantly higher than that of CsA. The changes of NOS and NO in skeletal muscle tissue will increase. Endurance training In practice, the NOS in the muscle tissue will increase and the production of NO is affected. CsA and endurance Under the influence of exercise, the change of NOS was not obvious and the content of NO increased.4. CsA intervention in that next group, the LDH content in the tissue increase and the CK change is restored to a quiet time . LDH recovery is slower than CK, and the time to reflect the damage is longer.5. Long-term endurance exercise, CsA intervention and the synergistic effect of both can cause Caveolin in the muscle of different fiber types. the reduction of the content of -3, affecting the signaling pathway mediated by the Caveolin-3. CsA is a slow representative of the soleus muscle
【學(xué)位授予單位】：遼寧師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：G804.2
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