【摘要】：目的:應用1H NMR代謝組學方法對優(yōu)秀龍舟運動員尿液代謝特征進行分析,結合尿常規(guī)進行全面、系統(tǒng)的訓練監(jiān)控。方法:于集訓期間及比賽前后連續(xù)5次收集運動員晨尿,采用1H NMR的代謝組學方法結合尿常規(guī)檢測,運用多元統(tǒng)計分析和t檢驗對實驗數據進行分析。結果:(1)與安靜時相比,男、女運動員尿液p H值、尿蛋白(PRO)和紅細胞(BLD)陽性檢出率分別在訓練期和比賽后具有顯著性變化(P0.05),特別是集訓1周和集訓2周時。(2)與安靜時相比,男運動員在不同訓練期和比賽后尿液中乳酸、谷氨酸、丙氨酸、谷氨酰胺、二甲胺和檸檬酸含量均顯著增加,而苯乙酰甘氨酸則明顯下降(P0.05);女運動員尿液中�；撬岷匡@著增加,苯乙酰甘氨酸同樣顯著下降(P0.05)。運動員尿液代謝組學指標主要在集訓1周、比賽次日和恢復1周時變化明顯。不同性別運動員尿液共有7種代謝物含量存在顯著性差異(P0.05)。結論:(1)龍舟運動能夠引起運動員機體內與糖、氨基酸代謝相關的代謝物發(fā)生顯著改變,運用1H NMR的代謝組學技術實現對運動員無損傷的訓練監(jiān)控可行。(2)不同性別運動員尿液代謝組學信息存在差異,可能由于訓練負荷安排和運動員機能狀態(tài)不同所致。(3)龍舟運動員尿液代謝組學信息比尿常規(guī)信息能更加敏感和全面地反映運動員機能狀態(tài),對運動性疲勞和過度訓練的監(jiān)控和早期診斷可能更有優(yōu)勢。
[Abstract]:Aim: to analyze the urine metabolic characteristics of elite dragon boat athletes by 1H NMR metabonomics, and to conduct comprehensive and systematic training monitoring combined with urine routine. Methods: the morning urine of athletes was collected 5 times during the training period and before and after the competition. The metabonomics method of 1H NMR combined with urine routine test was used to analyze the experimental data by multivariate statistical analysis and t test. Results: (1) there were significant changes in urine pH value, urine protein (PRO) and erythrocyte (BLD) positive rate between male and female athletes during training and after competition (P0.05). (2) the contents of lactic acid, glutamic acid, alanine, glutamine, dimethylamine and citric acid in urine of male athletes increased significantly in different training and post-match periods. However, phenylacetyl glycine decreased significantly (P0.05). Taurine content in urine of female athletes increased significantly, and phenylacetyl glycine also decreased significantly (P0.05). The urine metabonomics indexes of athletes mainly changed in one week of training, the second day of competition and one week of recovery. There were significant differences in the contents of seven metabolites in urine of athletes of different sexes (P0.05). Conclusion: (1) Dragon boat exercise can cause significant changes in metabolism of glucose and amino acids in athletes. It is feasible to use the metabonomics technology of 1H NMR to monitor the athletes without injury. (2) there are differences in the information of urine metabonomics among athletes of different sexes. (3) the information of urine metabonomics of dragon boat athletes can reflect the athletes' functional status more sensitively and comprehensively than the routine information of urine. Monitoring and early diagnosis of exercise-induced fatigue and overtraining may be more advantageous.
【作者單位】： 山西大學體育學院;
【基金】：中國博士后科學基金資助(2014M560197,2015T80234) 山西省教育廳“省高校重點實驗室”專項基金資助
【分類號】：R87

【參考文獻】

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1 冒海蕾;徐e，

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