【摘要】：一氧化氮（NO）自由基為一小分子氣體，以往對(duì)其生物學(xué)功能認(rèn)識(shí)不清。自從發(fā)現(xiàn)能舒張血管的一種內(nèi)皮細(xì)胞松弛因子（endothelium-derivedrelaxingfactor,EDRF）的化學(xué)本質(zhì)即一氧化氮自由基以來，一氧化氮自由基的研究進(jìn)入了一個(gè)新的時(shí)期。近幾十年來，一氧化氮自由基的研究進(jìn)展影響著對(duì)許多生命活動(dòng)本質(zhì)的認(rèn)識(shí)，涉及許多學(xué)科，，包括醫(yī)學(xué)和藥學(xué)等等，近年來更是擴(kuò)展到體育學(xué)科。不同運(yùn)動(dòng)負(fù)荷對(duì)一氧化氮及一氧化氮合酶的影響研究成為其中的熱點(diǎn)。不同研究方向的學(xué)者們都共同投入到對(duì)一氧化氮的研究中來，試圖從各個(gè)角度來揭示一氧化氮的奧秘。雖然運(yùn)動(dòng)醫(yī)學(xué)者們?cè)谝谎趸⑦\(yùn)動(dòng)與骨骼肌方面做了大量工作，但存有爭議的問題仍然很多。涉及到NOS的三種亞型與運(yùn)動(dòng)關(guān)系的研究更是爭議頗大，未有一個(gè)定論存在。 現(xiàn)有研究表明，運(yùn)動(dòng)導(dǎo)致NO生成，NOS活性發(fā)生變化，而力竭運(yùn)動(dòng)導(dǎo)致NOS活性升高的具體亞型尚不清楚，并且也有研究表明，骨骼肌中含有大量的nNOS，因此，本研究推測，力竭運(yùn)動(dòng)導(dǎo)致骨骼肌NOS活性升高主要是有nNOS活性升高所致。 研究方法：將32只大鼠隨機(jī)分成對(duì)照（C組）與訓(xùn)練（E組）兩組，進(jìn)行跑臺(tái)力竭運(yùn)動(dòng)，于運(yùn)動(dòng)結(jié)束后即刻取樣。 研究結(jié)果： （1）力竭訓(xùn)練后，大鼠血清CK活性升高33.81%（P0.05），而血清LDH下降7.59%，無顯著性差異（P0.05）。 （2）力竭訓(xùn)練后，大鼠血清NO顯著增加了16%（P0.05）。 （3）力竭訓(xùn)練后，大鼠骨骼肌MDA含量非常顯著性增加（P0.01），骨骼肌SOD活性顯著下降（P0.05）。 （4）力竭訓(xùn)練后，總NOS活性增加，有顯著性差異，大鼠骨骼肌nNOS活性非常顯著性升高（P0.01），iNOS活性升高，但無顯著性差異。 結(jié)論： （1）力竭運(yùn)動(dòng)導(dǎo)血清CK活性增高。 （2）力竭運(yùn)動(dòng)導(dǎo)致NOS活性增加，其根源在于nNOS活性增加，iNOS無變化；由于NOS活性增加，導(dǎo)致NO生成也增加。 （3）力竭運(yùn)動(dòng)導(dǎo)致脂質(zhì)過氧化程度加強(qiáng)。
[Abstract]:Nitric oxide (NO) radical is a small molecule gas and its biological function was not well understood in the past. Since the discovery of the chemical nature of an endothelial cell relaxation factor (endothelium-derivedrelaxingfactor,EDRF), the nitric oxide free radical, which can relax blood vessels, the study of nitric oxide free radical has entered a new stage. In recent decades, the research progress of nitric oxide free radicals has affected the understanding of the nature of many life activities, involving many disciplines, including medicine and pharmacy, etc. In recent years, it has been extended to physical education. The effects of different exercise loads on nitric oxide (no) and nitric oxide synthase (NOS) have become a hot topic. Scholars from different research fields have been involved in the study of nitric oxide, trying to reveal the mystery of nitric oxide from various angles. Although sports medicine has done a lot of work on nitric oxide, exercise and skeletal muscle, there are still many controversial issues. The research on the relationship between the three subtypes of NOS and sport is more controversial, and there is no conclusion. Current studies have shown that exercise causes NO production and changes in NOS activity, while the specific subtypes of exhaustive exercise that lead to increased NOS activity are not clear. Some studies have also shown that skeletal muscle contains a large number of nNOS,. The increase of NOS activity in skeletal muscle caused by exhaustive exercise was mainly due to the increase of nNOS activity. Methods: 32 rats were randomly divided into two groups: control group (group C) and training group (group E). Results: (1) after exhaustive training, the serum CK activity increased by 33.81% (P0.05), while the serum LDH decreased 7.59, there was no significant difference (P0.05). (2) after exhaustive training, serum NO increased significantly by 16% (P0.05). (3) after exhaustive training, the MDA content of skeletal muscle increased significantly (P0.01), and the SOD activity of skeletal muscle decreased significantly (P0.05). (4) after exhaustive training, the total NOS activity increased significantly, and the nNOS activity of skeletal muscle increased significantly (P0.01), iNOS activity, but there was no significant difference). Conclusion: (1) the activity of CK in exhaustive exercise induced serum increased. (2) exhaustive exercise resulted in the increase of NOS activity, which was caused by the increase of nNOS activity, but no change in iNOS activity, and the increase of NOS activity led to the increase of NO production. (3) exhaustive exercise increased the degree of lipid peroxidation.
【學(xué)位授予單位】：湖南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：G804.2

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