【摘要】：1研究目的 通過對大鼠心肌能量代謝相關指標的測定,探討不同強度的間歇跑臺訓練對大鼠能量代謝代謝相關酶和氧化基質的影響,以期為運動訓練強度的制定提供理論支持。 2實驗方法 選取4周齡雄性wistar大鼠70只(由山東魯抗醫(yī)藥股份有限公司提供)，平均體重為67.99g±5.83g,隨機分成7組,每組10只，即安靜對照組和運動組，運動組按運動訓練強度分為65%組、70%組、75%組、80%組、85%組和90%組。跑臺訓練9周，，每周訓練6天，每組訓練分3次，每次不低于10分鐘，中間間隔30分鐘，最后一次運動后24h，毀脊髓法處死后解剖，迅速取出心肌，在生理鹽水中漂洗干凈，置-84℃冰箱保存待測。制備組織勻漿分別測定大鼠心肌肌糖原含量、琥珀酸脫氫酶（SDH）、蘋果酸脫氫酶（MDH）、乳酸脫氫酶（LDH）、Ca~(2+)ATP酶、乳酸含量。所有數(shù)據(jù)用SPSS17.0軟件進行統(tǒng)計學處理。 3實驗結果 3.1大鼠心肌肌糖原含量的比較 80%組和85%組的心肌肌糖原含量顯著高于對照組（P＜0.05）；80%組和85%組的心肌肌糖原含量顯著高于65%組（P＜0.05）；對照組、65%組、70%組、75%組和90%組之間心肌肌糖原含量無顯著性差異。 3.2大鼠心肌SDH活性的比較 90%組的心肌SDH活性顯著高于對照組和65%組（P＜0.05）；對照組、65%組、70%組、75%組、80%組與85%組之間心肌SDH活性無統(tǒng)計學差異。 3.3大鼠心肌MDH活性的比較 65%組、70%組、75%組、80%組、85%組與90%組的心肌MDH活性均顯著高于對照組（P＜0.05）；85%組的心肌MDH活性最高，但運動組之間心肌MDH活性無顯著性差異。3.4大鼠心肌LDH活性的比較 65%組、70%組、75%組、80%組、85%組與90%組的心肌LDH活性均顯著高于對照組（P＜0.05）；90%組的心肌LDH活性顯著高于65%組和70%組（P＜0.05）；65%組、70%組、75%組、80%組與85%組的心肌LDH活性有增大的趨勢但相互之間無顯著差異性。 3.5大鼠心肌Ca~(2+)ATP酶活性的比較65%組、70%組、75%組、80%組與85%組的心肌Ca~(2+)ATP酶活性顯著高于對照組（P＜0.05）；85%組的心肌Ca~(2+)ATP酶活性最高；90%組心肌Ca~(2+)ATP酶略高于對照組，但無顯著性差異。 3.6大鼠心肌LD含量的比較90%組LD含量顯著高于對照組（P＜0.05）；其它各組之間無顯著性差異。 4結論 4.165%VO_2max、70%VO_2max和75%VO_2max的中等強度的跑臺訓練不能顯著地增加大鼠心肌肌糖原的含量，而85%VO_2max強度的長期訓練最能顯著地增加大鼠心肌肌糖原含量。90%VO_2max的長期跑臺訓練不利于大鼠心肌肌糖原的恢復及儲備。 4.2不同強度的跑臺訓練均能顯著提高大鼠心肌有氧代謝酶SDH、MDH的數(shù)量及活性，85%VO_2max的長期跑臺訓練最能提高大鼠心肌有氧代謝酶的活性，有利于心肌有氧代謝活動的發(fā)展。 4.3不同強度的跑臺訓練均能顯著提高大鼠心肌無氧代謝酶LDH的活性，90%VO_2maxLDH活性最高。90%VO_2max的長期跑臺訓練能使大鼠心肌產(chǎn)生肌糖原的無氧酵解供能，產(chǎn)生LD，且24小時后不能完全恢復。 4.465%VO_2max、70%VO_2max、75%VO_2max、80%VO_2max和85%VO_2max的長期跑臺運動能顯著提高大鼠心肌Ca~(2+)ATP酶的活性，85%VO_2maxCa~(2+)ATP酶的活性最高。90%VO_2max的長期跑臺訓練導致大鼠心肌代謝紊亂，不利于Ca~(2+)ATP酶的恢復。
[Abstract]:1 Purpose of study In order to provide theoretical support for the development of the intensity of exercise training, the effects of the intermittent running of different intensity on the metabolism of energy metabolism and the oxidative matrix of the rats were discussed by the determination of the related indexes of energy metabolism in the myocardium of the rats. hold. 2 The average body weight was 67. 99g (5.83g), and the average body weight was 67. 99g (5.83g), which was divided into 7 groups. In group and exercise group, the exercise group was divided into 65% group, 70% group, 75% group, 80% group and 85% group according to the exercise training intensity. and training for 9 weeks, training for 6 days per week, 3 times for each group, not less than 10 minutes at a time, 30 minutes for the middle interval, 24 hours after the last exercise, after the spinal cord is killed and then dissected, the myocardium is quickly removed, rinsed in normal saline, and the ice is set at -84 DEG C. The glycogen content, the succinate dehydrogenase (SDH), the malic acid dehydrogenase (MDH), the lactate dehydrogenase (LDH), the Ca ~ (2 +) ATPase in the myocardium of the rat were measured., lactic acid content. All data is performed with the SPSS17.0 software statistical processing. 3 Experimental results 3. 1 rat The glycogen content of the myocardium in the 80% and 85% group was significantly higher than that in the control group (P <0.05), and the glycogen content of the myocardial muscle in the 80% and the 85% group was significantly higher than that of the control group (P <0.05). Myocardium in 5% group (P <0.05); control group, 65% group, 70% group, 75% group and 90% group There was no significant difference in the content of glycogen in the muscle.. Compared with the control group and the 65% group (P <0.05), the activity of SDH in the myocardium of the rats was significantly higher than that in the control group (P <0.05), the control group, the 65% group, the 70% group, the 75% group, the 80% group and the 85% group. There was no statistical difference between the activity of SDH and SDH between the two groups.. 3. Compared with the control group (P <0), the activity of MDH in the myocardium of the rats in 65%, 70%, 75%, 80%, 85% and 90% were significantly higher than those in the control group (P <0).. 05) The MMDH activity in the 85% group was the highest, but the activity of MDH between the groups was not significant. A comparison of the activity of LDH in 3. 4 rats was 65%, 70%, 75%, 80%, 85% and 90% of the myocardium. The activity of LDH was significantly higher than that in the control group (P <0.05); the activity of LDH in the 90% group was significantly higher than that in the 65% group and 70% group (P <0.05); 65% group, 70% group, 75% group, 80% group and 85% group of myocardium L Compared with the control group (P <0.05), the activity of Ca ~ (2 +) ATPase in the myocardium of the rats was significantly higher than that in the control group (P <0.05). The activity of Ca ~ (2 +) ATPase in the group was higher than that in the control group (P <0.05). The activity of Ca ~ (2 +) ATPase in the group of 85% was the highest, and the Ca ~ (2 +) ATPase activity in the group was higher than that of the control group (P <0.05). ~ (2 +) ATPase was slightly higher than that in the control group, but there was no significant difference. in control 4. The long-term training of the intensity of 85% VO _ 2max could significantly increase the glycogen content of the rat cardiac muscle, while the long-term training of 85% VO _ 2max could increase the glycogen content of the myocardium of the rat significantly. The long-term training of O _ 2max is not conducive to the recovery and storage of the glycogen in the myocardium of the rat. The activity of the aerobic metabolizing enzyme in the myocardium of the rat can be improved, which is beneficial to the development of the activity of the aerobic metabolism of the myocardium. The results showed that the activity of Ca ~ (2 +) ATPase and the activity of 85% VO _ 2maxCa ~ (2 +) ATPase were the highest. The activity of Ca ~ (2 +) ATPase in rat myocardium was significantly increased by the long-term treadmill exercise of 4. 465% VO _ 2max, 70% VO _ 2max, 75% VO _ 2max, 80% VO _ 2max, and 85% VO _ 2max. The activity of 85% VO _ 2maxCa ~ (2 +) ATPase was the highest. 90% VO _ 2max
【學位授予單位】：曲阜師范大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R87

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