發(fā)布時(shí)間：2018-12-09 19:53

【摘要】：骨骼是人體的重要器官,具有重要的生理功能。與其他組織相同,骨組織處在一個(gè)動(dòng)態(tài)三維環(huán)境,其內(nèi)部的流體及溶質(zhì)運(yùn)輸對(duì)維持組織功能及本體活性具有不可替代的作用。目前光脫色熒光恢復(fù)技術(shù)(FRAP)是研究骨內(nèi)流體及溶質(zhì)運(yùn)輸?shù)闹饕侄?但由于光學(xué)成像技術(shù)的局限性,只能針對(duì)骨膜以下50μm區(qū)域進(jìn)行觀察分析。本文從流固耦合理論和有限元法出發(fā),對(duì)成年鼠脛骨進(jìn)行有限元仿真研究,并結(jié)合FRAP實(shí)驗(yàn)驗(yàn)證有限元法應(yīng)用于骨內(nèi)流體及溶質(zhì)運(yùn)輸研究的可行性。本文研究的主要內(nèi)容及結(jié)論包括：(1)利用二維CT斷層圖像及Mimics軟件平臺(tái),實(shí)現(xiàn)成年鼠脛骨的三維重建。在Hypermesh下對(duì)脛骨三維模型進(jìn)行網(wǎng)格劃分,三維重建后的網(wǎng)格模型與脛骨實(shí)物具有很高的相似度。(2)建立成年鼠脛骨的兩相-溶質(zhì)模型,獲得包括彈性模量、孔隙度、滲透率在內(nèi)的成年鼠脛骨模型相關(guān)參數(shù)。利用FEBio生物有限元仿真軟件,獲得了不同溶質(zhì)擴(kuò)散率以及載荷刺激下的骨陷窩-骨小管系統(tǒng)(LCS)內(nèi)溶質(zhì)輸運(yùn)特性。論述了輸運(yùn)增強(qiáng)度的概念,討論了擴(kuò)散率以及載荷對(duì)于輸運(yùn)增強(qiáng)度的影響。(3)結(jié)合成年鼠脛骨骨膜50μm以下區(qū)域的有限元分析及光脫色熒光恢復(fù)實(shí)驗(yàn)(FRAP)數(shù)據(jù),討論實(shí)驗(yàn)條件下載荷大小對(duì)于LCS系統(tǒng)內(nèi)溶質(zhì)輸運(yùn)現(xiàn)象的影響。比對(duì)同樣載荷條件下兩種方法得到的骨陷窩-骨小管系統(tǒng)內(nèi)溶質(zhì)輸運(yùn)增強(qiáng)度,驗(yàn)證生物有限元法在骨內(nèi)流體以及溶質(zhì)輸運(yùn)研究的可行性。(4)利用有限元法,進(jìn)一步研究骨內(nèi)深層區(qū)域流場(chǎng)分布信息,得到了不同載荷強(qiáng)度下脛骨表面以及橫斷面的流體壓力、流體流量、溶質(zhì)流量分布；并進(jìn)一步探討,孔隙度增大對(duì)于骨內(nèi)LCS系統(tǒng)流動(dòng)性影響。
[Abstract]:Bone is an important organ of human body and has important physiological function. Like other tissues, bone tissue is in a dynamic three-dimensional environment, and its internal fluid and solute transport plays an irreplaceable role in maintaining tissue function and bulk activity. At present, (FRAP) is the main method to study the transport of fluid and solute in bone. However, due to the limitation of optical imaging technology, only 50 渭 m below the periosteum can be observed and analyzed. Based on the fluid-solid coupling theory and finite element method, the finite element simulation of adult rat tibia is carried out, and the feasibility of applying the finite element method to the study of intraosseous fluid and solute transport is verified by FRAP experiment. The main contents and conclusions of this paper are as follows: (1) 3D reconstruction of adult rat tibia is realized by using two-dimensional CT tomography and Mimics software platform. The three-dimensional model of tibia was meshed by Hypermesh. The mesh model after 3D reconstruction had a high similarity with the tibia. (2) the two-phase solute model of adult rat tibia was established, which included elastic modulus and porosity. The related parameters of tibial model of adult rats included permeability. The transport characteristics of solute in bone lacunae bone tubule system (LCS) stimulated by different solute diffusivity and load were obtained by using FEBio biological finite element simulation software. The concept of transport enhancement is discussed, and the effects of diffusivity and load on transport enhancement are discussed. (3) combined with finite element analysis of tibial periosteum below 50 渭 m in adult rats and (FRAP) data of photochromic fluorescence recovery experiment, The effect of load on solute transport in LCS system is discussed. Comparing the enhancement degree of solute transport between the two methods under the same load conditions, the feasibility of the biological finite element method in the study of intraosseous fluid and solute transport is verified. (4) the finite element method is used to study the transport of solute in bone. The distribution of fluid pressure, fluid flow and solute flux on tibia surface and cross section under different loading intensity were obtained by further studying the distribution information of flow field in deep bone region. Furthermore, the effect of increased porosity on the flow of LCS system in bone was discussed.
【學(xué)位授予單位】：南京理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R816.8;R68

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本文編號(hào)：2369939