高脂膳食對(duì)大鼠心肌的脂毒性作用及有氧運(yùn)動(dòng)、原花青素干預(yù)效應(yīng)的實(shí)驗(yàn)研究
發(fā)布時(shí)間：2018-11-05 13:01
【摘要】：目的:通過對(duì)高脂膳食聯(lián)合有氧運(yùn)動(dòng)以及補(bǔ)充原花青素大鼠血漿FFA、IL-6、TNF-α水平變化和心肌TLR4、NF-κBm RNA表達(dá)量、FFA、ICAM-1含量變化的分析,探討高脂膳食對(duì)大鼠心肌的脂毒性作用及有氧運(yùn)動(dòng)聯(lián)合補(bǔ)充原花青素對(duì)高脂膳食大鼠心肌的保護(hù)作用。方法:8周齡健康SD大鼠50只,體重180-220克。適應(yīng)性喂養(yǎng)1周后隨機(jī)分為5組:對(duì)照組(N=10,C)、高脂膳食組(N=10,H)、高脂膳食運(yùn)動(dòng)組(N=10,HE)、高脂膳食補(bǔ)充原花青素組(N=10,HG)、高脂膳食運(yùn)動(dòng)補(bǔ)充原花青素組(N=10,HEG)。HE、HEG組進(jìn)行有氧運(yùn)動(dòng),均采用0坡度跑臺(tái),第一周15m/min,第二周20m/min,以后每周增加1m/min,運(yùn)動(dòng)50min/次。運(yùn)動(dòng)時(shí)間:21:00-24:00,1次/天,6天/周,共6周。HG、HEG組每運(yùn)動(dòng)前半小時(shí)按200mg/kg體重原花青素灌胃,其余組等量等體積生理鹽水灌胃。每周固定時(shí)間測(cè)量記錄各組體重體長數(shù)據(jù)。6周實(shí)驗(yàn)結(jié)束后,ELISA法檢測(cè)各組大鼠血漿FFA、IL-6、TNF-α水平和心肌FFA、ICAM-1的含量,RT-PCR方法測(cè)試心肌NF-κBm RNA表達(dá)水平,Western blot法測(cè)TLR4蛋白表達(dá)量。運(yùn)用SPSS17.0軟件處理數(shù)據(jù)。結(jié)果:1.H組大鼠Lee’s指數(shù)、血漿FFA、TNF-α、IL-6含量和心肌TLR4、NF-κBm RNA表達(dá)水平及FFA、ICAM-1含量明顯高于C組(P0.01)。2.HE、HG、HEG組大鼠Lee’s指數(shù)、血漿FFA、TNF-ɑ含量明顯低于H組(P0.01、P0.05),血漿IL-6水平無顯著性變化(P0.05);有氧運(yùn)動(dòng)、補(bǔ)充原花青素對(duì)Lee’s指數(shù)、血漿FFA、TNF-ɑ含量的影響無顯著交互作用(P0.05)。3.HE、HG、HEG組大鼠心肌ICAM-1含量與H組相比無顯著差異(P0.05),有氧運(yùn)動(dòng)、補(bǔ)充原花青素對(duì)心肌ICAM-1含量的影響無顯著交互作用(P0.05)。4.HE、HG、HEG組大鼠心肌FFA、TLR4蛋白含量和NF-кBm RNA表達(dá)量明顯低于H組(P0.05、P0.01);有氧運(yùn)動(dòng)、補(bǔ)充原花青素對(duì)心肌FFA、TLR4蛋白含量和NF-кBm RNA表達(dá)量的影響有顯著交互作用(P0.05);在相同補(bǔ)充原花青素的條件下,運(yùn)動(dòng)可使大鼠FFA含量明顯降低(P0.05);相同運(yùn)動(dòng)條件下,補(bǔ)充原花青素可使大鼠TLR4、NF-кBm RNA表達(dá)量明顯降低(P0.01)。結(jié)論:1.6周高脂膳食可使大鼠體重和Lee’s指數(shù)增加,血漿FFA、TNF-ɑ、IL-6水平不同程度的升高,誘發(fā)機(jī)體脂毒性和炎癥反應(yīng)。2.6周高脂膳食可使大鼠心肌FFA、TLR4和ICAM-1含量增加、NF-κBm RNA表達(dá)上調(diào),誘發(fā)心肌脂毒性和炎癥反應(yīng)以及心肌血管內(nèi)皮損傷。3.6周有氧運(yùn)動(dòng)和補(bǔ)充原花青素具有減輕高脂膳食大鼠肥胖、緩解其機(jī)體和心肌脂毒性及炎癥反應(yīng)作用,從而保護(hù)心肌;有氧運(yùn)動(dòng)聯(lián)合補(bǔ)充原青花素對(duì)心肌的保護(hù)作用比較單一有氧運(yùn)動(dòng)或補(bǔ)充原青花素效果更優(yōu)。
[Abstract]:Objective: to analyze the changes of plasma FFA,IL-6,TNF- 偽 level and myocardial TLR4,NF- 魏 Bm RNA expression and FFA,ICAM-1 content in rats with high fat diet combined with aerobic exercise and procyanidin supplementation. To investigate the effects of hyperlipidemic diet on myocardial lipid toxicity in rats and the protective effects of aerobic exercise combined with procyanidin supplementation on myocardium of rats with high fat diet. Methods: 50 healthy 8-week SD rats with body weight of 180-220 g were used. After one week of adaptive feeding, the rats were randomly divided into five groups: control group (n = 10), high fat diet group (n = 10) and proanthocyanidin supplement group (n = 10 HG). High fat diet supplemented with proanthocyanidin (NC10). The HE,HEG group performed aerobic exercise with 0 slope running platform, 15 m / min in the first week, 20 m / min in the second week, and 1 m / min, 1 m / min, 50min/ times per week thereafter. Exercise time: 21: 00-24: 00 once a day, 6 days a week for 6 weeks. The HG,HEG group was gavaged with 200mg/kg proanthocyanidins half an hour before exercise, and the other groups were given the same volume of normal saline. The body weight and body length of each group were measured at a fixed time every week. At the end of the 6-week experiment, the plasma FFA,IL-6,TNF- 偽 level and myocardial FFA,ICAM-1 content were measured by ELISA method. The expression level of NF- 魏 Bm RNA in myocardium was measured by RT-PCR method. The expression of TLR4 protein was measured by, Western blot method. Use SPSS17.0 software to process data. Results: 1. The Lee's index, plasma FFA,TNF- 偽, IL-6 content, myocardial TLR4,NF- 魏 Bm RNA expression and FFA,ICAM-1 content in H group were significantly higher than those in C group (P0.01). 2. The level of plasma FFA,TNF- was significantly lower than that of group H (P 0.01 P 0.05), but the level of plasma IL-6 had no significant change (P 0.05). There was no significant interaction between aerobic exercise and procyanidin supplementation on Lee's index and plasma FFA,TNF- content (P0.05). 3. There was no significant difference in myocardial ICAM-1 content between HGG group and H group (P0.05). The effects of aerobic exercise and proanthocyanidin supplementation on myocardial ICAM-1 content were not significant (P0.05). 4. The content of FFA,TLR4 protein and the expression of NF- Bm RNA in myocardium of HEHG group were significantly lower than that of H group (P0.05). P0.01); The effects of aerobic exercise and proanthocyanidin supplementation on myocardial FFA,TLR4 protein content and NF- Bm RNA expression were significant (P0.05). Under the same condition of supplementation of proanthocyanidins, the content of FFA in rats was significantly decreased by exercise (P0.05), and the expression of TLR4,NF- Bm RNA in rats was significantly decreased by supplementation of proanthocyanidins under the same exercise conditions (P0.01). Conclusion: the high fat diet of 1.6 weeks can increase the body weight and Lee's index of rats, and increase the levels of plasma FFA,TNF- and IL-6 in varying degrees, which can induce lipid toxicity and inflammatory reaction. 2.6-week high fat diet can induce myocardial FFA, in rats. The contents of TLR4 and ICAM-1 were increased, the expression of NF- 魏 Bm RNA was upregulated, myocardial lipotoxicity and inflammation were induced, and myocardial vascular endothelial injury was induced. Aerobic exercise and proanthocyanidin supplementation at 3.6 weeks could reduce obesity in high-fat diet rats. Alleviating the lipid toxicity and inflammatory reaction of the body and myocardium, thereby protecting the myocardium; The protective effect of aerobic exercise combined with procyanidin on myocardium was better than that of single aerobic exercise or proanthocyanidin supplementation.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R87
[Abstract]:Objective: to analyze the changes of plasma FFA,IL-6,TNF- 偽 level and myocardial TLR4,NF- 魏 Bm RNA expression and FFA,ICAM-1 content in rats with high fat diet combined with aerobic exercise and procyanidin supplementation. To investigate the effects of hyperlipidemic diet on myocardial lipid toxicity in rats and the protective effects of aerobic exercise combined with procyanidin supplementation on myocardium of rats with high fat diet. Methods: 50 healthy 8-week SD rats with body weight of 180-220 g were used. After one week of adaptive feeding, the rats were randomly divided into five groups: control group (n = 10), high fat diet group (n = 10) and proanthocyanidin supplement group (n = 10 HG). High fat diet supplemented with proanthocyanidin (NC10). The HE,HEG group performed aerobic exercise with 0 slope running platform, 15 m / min in the first week, 20 m / min in the second week, and 1 m / min, 1 m / min, 50min/ times per week thereafter. Exercise time: 21: 00-24: 00 once a day, 6 days a week for 6 weeks. The HG,HEG group was gavaged with 200mg/kg proanthocyanidins half an hour before exercise, and the other groups were given the same volume of normal saline. The body weight and body length of each group were measured at a fixed time every week. At the end of the 6-week experiment, the plasma FFA,IL-6,TNF- 偽 level and myocardial FFA,ICAM-1 content were measured by ELISA method. The expression level of NF- 魏 Bm RNA in myocardium was measured by RT-PCR method. The expression of TLR4 protein was measured by, Western blot method. Use SPSS17.0 software to process data. Results: 1. The Lee's index, plasma FFA,TNF- 偽, IL-6 content, myocardial TLR4,NF- 魏 Bm RNA expression and FFA,ICAM-1 content in H group were significantly higher than those in C group (P0.01). 2. The level of plasma FFA,TNF- was significantly lower than that of group H (P 0.01 P 0.05), but the level of plasma IL-6 had no significant change (P 0.05). There was no significant interaction between aerobic exercise and procyanidin supplementation on Lee's index and plasma FFA,TNF- content (P0.05). 3. There was no significant difference in myocardial ICAM-1 content between HGG group and H group (P0.05). The effects of aerobic exercise and proanthocyanidin supplementation on myocardial ICAM-1 content were not significant (P0.05). 4. The content of FFA,TLR4 protein and the expression of NF- Bm RNA in myocardium of HEHG group were significantly lower than that of H group (P0.05). P0.01); The effects of aerobic exercise and proanthocyanidin supplementation on myocardial FFA,TLR4 protein content and NF- Bm RNA expression were significant (P0.05). Under the same condition of supplementation of proanthocyanidins, the content of FFA in rats was significantly decreased by exercise (P0.05), and the expression of TLR4,NF- Bm RNA in rats was significantly decreased by supplementation of proanthocyanidins under the same exercise conditions (P0.01). Conclusion: the high fat diet of 1.6 weeks can increase the body weight and Lee's index of rats, and increase the levels of plasma FFA,TNF- and IL-6 in varying degrees, which can induce lipid toxicity and inflammatory reaction. 2.6-week high fat diet can induce myocardial FFA, in rats. The contents of TLR4 and ICAM-1 were increased, the expression of NF- 魏 Bm RNA was upregulated, myocardial lipotoxicity and inflammation were induced, and myocardial vascular endothelial injury was induced. Aerobic exercise and proanthocyanidin supplementation at 3.6 weeks could reduce obesity in high-fat diet rats. Alleviating the lipid toxicity and inflammatory reaction of the body and myocardium, thereby protecting the myocardium; The protective effect of aerobic exercise combined with procyanidin on myocardium was better than that of single aerobic exercise or proanthocyanidin supplementation.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R87
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