【摘要】：目的建立27個(gè)常染色體SNPs復(fù)合檢測(cè)體系,用于未知個(gè)體種族來源推斷。方法通過對(duì)Hap Map數(shù)據(jù)庫中描述祖先(非洲、歐洲、東亞)的遺傳標(biāo)記信息分析,選出27個(gè)SNPs位點(diǎn),構(gòu)建27個(gè)SNPs復(fù)合擴(kuò)增體系;采用該體系對(duì)17個(gè)不同祖先人群的1 164份樣本進(jìn)行測(cè)試,得到的分型數(shù)據(jù)和在Hap Map數(shù)據(jù)庫查詢到的11個(gè)相關(guān)人群的數(shù)據(jù);采用據(jù)聚類分析方法(K=3)進(jìn)行祖先成分和匹配率計(jì)算,分析推算樣品9947A的祖先來源,并進(jìn)行體系性能驗(yàn)證。結(jié)果該體系可以進(jìn)行單一和混合人群的種族來源和種族成分推斷,來自新疆的人群遺傳成分呈現(xiàn)在歐洲與東亞祖先之間連續(xù)分布,樣品9947A祖先成分和匹配率與相關(guān)文獻(xiàn)分析結(jié)果一致。濃度最低為0.1ng/μL時(shí)27個(gè)等位基因均可正確判型。結(jié)論本文構(gòu)建的27-plex SNPs復(fù)合體系可以精確推斷非洲、歐洲、東亞血統(tǒng)的個(gè)體祖先起源,且對(duì)歐亞混合人群(歐洲/東亞)有較好的推斷能力,可在相關(guān)研究和實(shí)踐中選擇使用。
[Abstract]:Objective to establish 27 autosomal SNPs complex detection systems for ethnic origin inference of unknown individuals. Methods 27 SNPs loci were selected by analyzing the genetic markers in Hap Map database describing ancestors (Africa, Europe, East Asia), and 27 SNPs multiplex amplification systems were constructed. Using this system, 1 164 samples from 17 different ancestor populations were tested, and 11 related population data were obtained from Hap Map database. The ancestral composition and matching rate were calculated by cluster analysis method (KK3). The ancestral origin of the calculated sample 9947A was analyzed and the system performance was verified. Results the system can infer the ethnic origin and ethnic composition of a single and mixed population, and the genetic components of the population from Xinjiang are continuously distributed between the ancestors of Europe and East Asia. Sample 9947A ancestor composition and matching rate are consistent with the results of related literature analysis. The 27 alleles with the lowest concentration of 0.1ng/ 渭 L could be correctly identified. Conclusion the 27-plex SNPs composite system constructed in this paper can accurately infer the origin of individual ancestors of African, European and East Asian ancestry, and has a good ability to infer the Eurasian mixed population (Europe / East Asia). Can be used in relevant research and practice.
【作者單位】： 公安部物證鑒定中心法醫(yī)遺傳學(xué)公安部重點(diǎn)實(shí)驗(yàn)室;公安部物證鑒定中心北京市現(xiàn)場(chǎng)物證檢驗(yàn)工程技術(shù)研究中心;山西醫(yī)科大學(xué);天津醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院免疫系;
【基金】：中央級(jí)公益性基本科研業(yè)務(wù)費(fèi)項(xiàng)目(2015JB023) 十二五課題支撐項(xiàng)目(2012BAK02B01)
【分類號(hào)】：D919.4
，

本文編號(hào)：2295789