【摘要】：目的探討肺功能分析在放射性肺損傷防治藥物藥效研究中的意義。方法采用30只C57BL/6小鼠以20 Gy的X線行小鼠胸部單次照射,照射后給予地塞米松[給藥組按4.5 mg/(kg·d)的劑量給予地塞米松磷酸鈉注射液,2周后減半維持至1個(gè)月],對(duì)照組和模型組給予等體積生理鹽水。照射后1個(gè)月,使用EMKA肺功能分析系統(tǒng)在清醒狀態(tài)下行肺功能測試,隨后活殺取材,HE染色觀察肺組織結(jié)構(gòu)變化,應(yīng)用Image-pro圖像分析軟件分析肺泡腔面積。結(jié)果給藥1個(gè)月后,模型組及地塞米松組小鼠肺功能部分指標(biāo),如50%呼氣流量、每分通氣量、呼氣峰值、吸氣峰值、潮氣量較對(duì)照組明顯降低,但地塞米松組較模型組降低程度有所減輕。肺組織病理變化示,地塞米松組小鼠肺泡腔面積減小程度、肺間質(zhì)中性粒細(xì)胞浸潤程度均較模型組有所減輕,與肺功能分析結(jié)果相符。結(jié)論地塞米松對(duì)放射性肺損傷具有一定治療效果;肺功能分析與肺組織病理變化相結(jié)合印證,可以有效評(píng)價(jià)放射性肺損傷防治藥物的藥效。
[Abstract]:Objective to explore the significance of pulmonary function analysis in the study of drug efficacy in prevention and treatment of radiation lung injury. Methods A total of 30 C57BL/6 mice were exposed to single chest irradiation for 20 Gy. After irradiation, dexamethasone [dexamethasone sodium phosphate injection was given to the control group at a dose of 4.5 mg/ (kg d), maintained by half to 1 month after 2 weeks], and the control group and the model group were given the same volume of normal saline. One month after irradiation, the lung function was tested by EMKA pulmonary function analysis system in awake state, and then the lung tissue structure was observed by HE staining. The alveolar area was analyzed by Image-pro image analysis software. Results one month after administration, some indexes of lung function in model group and dexamethasone group were significantly lower than those in control group, such as 50% expiratory flow, per minute ventilation, peak expiratory peak, inspiratory peak and tidal volume. However, the decrease of dexamethasone group was less than that of model group. The pathological changes of lung tissue showed that the area of alveolar cavity and the infiltration of interstitial neutrophil in dexamethasone group were decreased compared with those in the model group, which were consistent with the results of pulmonary function analysis. Conclusion Dexamethasone has a certain therapeutic effect on radiation-induced lung injury and the combination of lung function analysis and pathological changes of lung tissue can effectively evaluate the efficacy of radiation-induced lung injury prevention and treatment drugs.
【作者單位】： 安徽醫(yī)科大學(xué)研究生學(xué)院;軍事醫(yī)學(xué)科學(xué)院放射與輻射醫(yī)學(xué)研究所;北京諾斯蘭德生物技術(shù)股份有限公司;中國人民解放軍總參總醫(yī)院放療科;
【基金】：北京市自然科學(xué)基金資助項(xiàng)目(7132149)
【分類號(hào)】：R818

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