【摘要】：為研究γ電離輻射對水生生物重要器官發(fā)育形態(tài)學(xué)的影響,以斑馬魚(Danio rerio)作為模式生物,研究了胚胎發(fā)育早期(受精后5h,5hpf)接受急性γ輻照(累積劑量為0.25、0.5、1Gy,劑量率為47.79cGy/min)后,受精后3d(3dpf)的幼魚卵黃囊面積以及3dpf和5dpf的幼魚肝、腎和脾發(fā)育形態(tài)學(xué)的變化。結(jié)果顯示:3dpf的幼魚卵黃囊面積隨劑量的增大而增大;3dpf的幼魚肝臟中空泡增加、肝臟局部出現(xiàn)空洞;5dpf的幼魚肝臟尺寸縮減、肝細(xì)胞內(nèi)空泡增加且細(xì)胞核變形、血竇較小以及有核紅細(xì)胞變形等;但前腎和脾的發(fā)育形態(tài)并沒有出現(xiàn)異常。隨后對3dpf的幼魚肝細(xì)胞超微結(jié)構(gòu)進(jìn)行觀察,發(fā)現(xiàn)γ輻照使幼魚肝臟細(xì)胞線粒體受損、核膜間隙擴(kuò)大、粗面內(nèi)質(zhì)網(wǎng)排列混亂等。以上結(jié)果表明,γ輻照對胚胎卵黃吸收有抑制作用,并呈一定的劑量依賴性,胚胎肝臟的發(fā)育形態(tài)在顯微及亞顯微結(jié)構(gòu)上都發(fā)生了改變。
[Abstract]:In order to study the effect of 緯 ionizing radiation on the development morphology of important organs of aquatic organisms, zebrafish (Danio rerio) was used as model organism to study the acute 緯 irradiation (cumulative dose 0.25g 0.51Gy, dose rate 47.79cGy/min) in early embryonic development (5hu 5hpf after fertilization). The area of yolk sac of young fish (3dpf) and the morphologic changes of liver kidney and spleen of 3dpf and 5dpf were observed on the 3rd day after fertilization. The results showed that the area of yolk sac increased with the increase of dose, the vacuole of liver of 3 dpf increased, the size of liver of 5dpf decreased, the vacuole of liver cells increased and the nucleus of liver deformed. The antrum was small and the nucleated red blood cells deformed, but the shape of the anterior kidney and spleen was not abnormal. The ultrastructure of hepatocytes of 3dpf was observed. It was found that 緯 irradiation damaged mitochondria, enlarged nuclear membrane space and confused rough endoplasmic reticulum arrangement. The results showed that 緯 irradiation inhibited yolk absorption in a dose-dependent manner, and the developmental morphology of the embryonic liver changed in both microscopic and submicroscopic structures.
【作者單位】： 南華大學(xué)鈾礦冶生物技術(shù)國防重點(diǎn)學(xué)科實(shí)驗(yàn)室;
【基金】：國防基礎(chǔ)重點(diǎn)基金資助項(xiàng)目(B3720132001) 國家自然科學(xué)基金重點(diǎn)項(xiàng)目資助(U1401231) 國防基礎(chǔ)科研計(jì)劃資助項(xiàng)目(JCKY2016403C001)
【分類號】：R818
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本文編號：2261249