【摘要】：目的：研究不同劑量C060γ射線照射大鼠涎腺組織后Rad50蛋白的表達變化情況及其對涎腺組織凋亡的影響。 方法：選取近交系雄性Wistar大鼠60只,按照隨機數(shù)字表法分成6組：對照組(未進行放射,即0Gy組),放射組3Gy組、6Gy組、9Gy組、12Gy組、15Gy組,每組10只。每次3Gy,隔天一次,累積劑量分別為3Gy組、6Gy組、9Gy組、12Gy組、15Gy。并在2h后處死大鼠,收集大鼠腮腺及頜下腺組織。使用免疫印跡法檢測Rad50蛋白在大鼠腮腺、頜下腺的表達情況；用TUNNEL法檢測放射后大鼠涎腺組織的細胞凋亡情況。 結果：免疫印跡法檢測顯示,放射后大鼠在腮腺組織及頜下腺組織中的Rad50蛋白的表達量隨著放射劑量的不同而改變；放射組與正常組比較,腮腺組織Rad50蛋白在9Gy(P0.05),頜下腺組織在12Gy(P0.05)達到最高。隨著放射劑量的增加,Funel檢測法檢出陽性率增加。當放射劑量為9Gy(P0.05)時,腮腺組織中細胞陽性率最高；放射劑量為12Gy(P0.05)時,頜下腺組織中的陽性率最高。放射劑量對細胞凋亡陽性率有顯著的影響,陽性率的變化與劑量相關。 結論：Rad50蛋白的表達水平受放射劑量和組織類型的影響,可能參與了涎腺放射損傷修復的機制。涎腺組織中腮腺的放射敏感性高于頜下腺組織。
[Abstract]:Aim: to study the changes of Rad50 protein expression in salivary gland of rats irradiated with different doses of C060 緯-ray and its effect on salivary gland apoptosis. Methods: sixty inbred male Wistar rats were randomly divided into 6 groups: control group (no radiation, 0Gy group), 3Gy group, 3Gy group, 12 Gy group, 15 Gy group, 10 rats in each group. The cumulative doses were 15 Gy in 3Gy group, 9 Gy in 9 Gy group and 12 Gy in 12 Gy group, respectively. The parotid gland and submandibular gland of the rats were collected after 2 hours. The expression of Rad50 protein in parotid gland and submandibular gland was detected by Western blot, and apoptosis in salivary gland was detected by TUNNEL assay. Results: Western blot assay showed that the expression of Rad50 protein in parotid and submandibular gland tissues changed with the dose of radiation, and the expression of Rad50 protein in the radiation group was higher than that in the normal group. Rad50 protein in parotid tissue was highest in 9Gy (P0.05), and in submandibular gland in 12Gy (P0.05). With the increase of radiation dose, the positive rate of Funel test increased. When the radiation dose was 9Gy (P0.05), the positive rate of cells in parotid gland was the highest; when the radiation dose was 12Gy (P0.05), the positive rate of submandibular gland was the highest. Radiation dose had a significant effect on the positive rate of apoptosis, and the change of the positive rate was correlated with the dose. Conclusion the expression of the protein is affected by radiation dose and tissue type, which may play a role in the repair of salivary gland injury. The radiosensitivity of parotid gland in salivary gland was higher than that in submandibular gland.
【學位授予單位】：廣西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R818.74

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