【摘要】：背景:同步輻射相位襯度成像憑借硬X射線光源的高準直、高相干等特性,其能大幅度提高對軟骨細胞及微血管的空間分辨能力。目的:利用高空間分辨率同步輻射相襯成像技術(shù)觀察大鼠正常及急性損傷脊髓的微血管的形態(tài)變化。方法:雄性SD大鼠分為2組:實驗組以改良ALLEN’s打擊法制作大鼠急性脊髓挫傷模型;偽手術(shù)組大鼠只行椎板減壓術(shù),不打擊脊髓作為正常對照。術(shù)后第1天取正常及傷段脊髓標本經(jīng)甲醛-水楊酸甲酯序貫處理48 h,標本于上海光源BL13W1硬X射線站進行掃描和成像。采集數(shù)據(jù)以利用VG Studio Max 2.1軟件包進行3D圖形重建及血管量化分析。結(jié)果與結(jié)論:相位襯度成像以特有的血管邊緣增強效應(yīng)直觀呈現(xiàn)脊髓微血管形態(tài),其結(jié)合CT技術(shù)(相襯斷層成像)從三維視角對脊髓微血管進行直觀成像,急性脊髓挫傷后神經(jīng)組織的破壞伴隨著嚴重的血管結(jié)構(gòu)的損毀,組織損傷及血供的缺失在髓內(nèi)由中央?yún)^(qū)向頭、尾兩側(cè)呈梭性蔓延。三維血管量化數(shù)據(jù)顯示脊髓挫傷后微血管數(shù)目及血管灌注容積急劇減少(P0.01)。結(jié)果表明無需血管造影的條件下,相襯斷層成像可作為一種新的有潛力的超高分辨率可視化技術(shù)用于脊髓微血管3D結(jié)構(gòu)成像及量化分析。
[Abstract]:Background: synchrotron radiation phase contrast imaging can greatly improve the spatial resolution of chondrocytes and microvessels by virtue of the high collimation and coherence of hard X-ray sources. Aim: to observe the morphologic changes of microvessels in normal and acute injured spinal cord by high spatial resolution synchrotron radiation contrast imaging. Methods: male SD rats were divided into two groups: the experimental group was made into acute spinal cord contusion model by modified ALLEN's attack method, and the pseudo-operation group was treated with laminar decompression only, without striking the spinal cord as the normal control. The normal and injured spinal cord samples were sequentially treated with formaldehyde-methyl salicylate for 48 hours on the first day after operation. The specimens were scanned and imaged at BL13W1 hard X-ray station of Shanghai Light Source. Data were collected for 3D graphics reconstruction and vascular quantitative analysis using VG Studio Max 2.1 software package. Results and conclusion: phase contrast imaging can visualize the morphology of spinal microvessels with the characteristic edge enhancement effect. The phase contrast imaging combined with CT (phase contrast tomography) is used to visualize the spinal microvessels from a three-dimensional perspective. The damage of nerve tissue after acute spinal cord contusion was accompanied by serious damage of vascular structure. The injury of tissue and the loss of blood supply spread from the central area to the head of the medulla and the two sides of the tail spread fusiform. Three dimensional vascular quantitative data showed that the number of microvessels and the volume of vascular perfusion decreased significantly after spinal cord contusion (P0.01). The results show that phase contrast tomography can be used as a potential ultra-high resolution visualization technique for 3D structure imaging and quantitative analysis of spinal microvessels without angiography.
【作者單位】： 中南大學湘雅醫(yī)院脊柱外科;中南大學湘雅醫(yī)院運動醫(yī)學科運動醫(yī)學研究中心;
【基金】：國家自然科學基金(81301542,81371956)~~
【分類號】：R651.2;R816.1

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