【摘要】：目的觀察4周跑臺(tái)運(yùn)動(dòng)對(duì)慢性應(yīng)激致去卵巢大鼠抑郁、焦慮行為及空間學(xué)習(xí)記憶能力和前額葉皮質(zhì)(Prefrontal Cortex,PFC)單胺類神經(jīng)遞質(zhì)水平及腦源性神經(jīng)營養(yǎng)因子(Brain-Derived Neurotrophic Factor,BDNF)表達(dá)的影響。方法 40只SD大鼠隨機(jī)分為假手術(shù)組、去卵巢組、去卵巢應(yīng)激組及去卵巢應(yīng)激運(yùn)動(dòng)組,除了假手術(shù)組大鼠,其余3組大鼠采用卵巢摘除建立大鼠圍絕經(jīng)期模型,再通過慢性不可預(yù)知性應(yīng)激(chronic unpredictable stress,CUMS)建立抑郁模型,同時(shí),應(yīng)激運(yùn)動(dòng)組大鼠進(jìn)行4周跑臺(tái)運(yùn)動(dòng)。運(yùn)動(dòng)及應(yīng)激結(jié)束后通過曠場(chǎng)實(shí)驗(yàn)、高架迷宮實(shí)驗(yàn)、明暗箱探索實(shí)驗(yàn)及水迷宮實(shí)驗(yàn)等方法測(cè)試大鼠行為學(xué)指標(biāo)變化,采用高效液相-電化學(xué)檢測(cè)法檢測(cè)前額葉皮質(zhì)去甲腎上腺素(norepinephrine,NE)、多巴胺(dopamine,DA)及5-羥色胺(5-hydroxy trptamine,5-HT)的含量,用免疫組織化學(xué)結(jié)合圖像半定量方法對(duì)前額葉皮質(zhì)BDNF神經(jīng)元的數(shù)量、面積進(jìn)行測(cè)量和分析。結(jié)果卵巢摘除及慢性不可預(yù)知應(yīng)激增強(qiáng)了大鼠的抑郁行為及焦慮行為,學(xué)習(xí)記憶能力下降,行為學(xué)表現(xiàn)為曠場(chǎng)試驗(yàn)中大鼠穿越格數(shù)及直立次數(shù)均顯著減少,高架迷宮實(shí)驗(yàn)中OE%、OT%及(開放臂+閉合臂)均顯著下降,明暗箱實(shí)驗(yàn)中大鼠明箱時(shí)間縮短,水迷宮實(shí)驗(yàn)中學(xué)習(xí)記憶能力下降;血清皮質(zhì)醇(cortisol,Cort)水平增加,前額葉皮質(zhì)單胺類神經(jīng)遞質(zhì)DA、NE、5-HT含量均顯著下降,BDNF表達(dá)減弱。而長(zhǎng)期跑臺(tái)運(yùn)動(dòng)可以顯著改善去卵巢抑郁模型大鼠的抑郁樣行為及增強(qiáng)學(xué)習(xí)記憶能力,血清Cort水平下降,前額葉皮質(zhì)單胺類神經(jīng)遞質(zhì)DA、NE、5-HT含量增加,BDNF表達(dá)增強(qiáng)。結(jié)論跑臺(tái)運(yùn)動(dòng)可改善去卵巢抑郁模型大鼠的抑郁及焦慮行為,增強(qiáng)大鼠學(xué)習(xí)記憶能力,機(jī)理可能與長(zhǎng)期跑臺(tái)運(yùn)動(dòng)降低去卵巢抑郁大鼠Cort水平、拮抗HPA軸功能亢進(jìn)、上調(diào)前額葉皮質(zhì)BDNF的表達(dá)、有效調(diào)整中樞神經(jīng)遞質(zhì)紊亂有關(guān)。
[Abstract]:Objective to observe the effects of treadmill exercise on depression, anxiety behavior, spatial learning and memory ability, monoamine neurotransmitter level in Prefrontal Cortexa cortex and the expression of Brain-Derived Neurotrophic factorial neurotrophic factor (BDNF) in ovariectomized rats induced by chronic stress. Methods Forty SD rats were randomly divided into three groups: ovariectomized stress group, ovariectomized stress group and ovariectomized exercise group. The depression model was established by chronic unpredictable stress (chronic unpredictable stress CUMS). At the same time, the rats in the stress exercise group underwent treadmill exercise for 4 weeks. After exercise and stress, the behavioral indexes of rats were measured by open field experiment, elevated maze test, open and dark box exploration experiment and water maze test. The contents of norepinephrine (NE), dopamine (DA) and 5-hydroxy trptamine 5-HT (5-HT) in prefrontal cortex were detected by high performance liquid chromatography (HPLC) and electrochemical method. The number of BDNF neurons in prefrontal cortex was determined by immunohistochemistry and image semi-quantitative method. The area is measured and analyzed. Results Ovariectomized and chronic unpredictable stress enhanced the depression behavior and anxiety behavior, decreased learning and memory ability, and significantly decreased the number of traversals and the number of upright rats in open field test. In the elevated labyrinth experiment, the OEO% and open arm closed arm decreased significantly, the open box time shortened, the learning and memory ability decreased and the serum cortisol level increased. The expression of BDNF decreased significantly in prefrontal cortex monoamine neurotransmitters. Long-term treadmill exercise could significantly improve the depressive behavior and enhance the learning and memory ability of ovariectomized depression rats. The serum Cort level decreased and the prefrontal cortex monoamine neurotransmitter DANE-5-HT increased. Conclusion treadmill exercise can improve the depression and anxiety behavior of ovariectomized depression rats and enhance the learning and memory ability of the rats. The mechanism may be associated with long-term treadmill exercise to reduce the level of Cort and antagonize the hyperfunction of HPA axis in ovariectomized depression rats. The up-regulation of BDNF expression in prefrontal cortex is related to the regulation of central neurotransmitter disorder.
【作者單位】： 山西醫(yī)科大學(xué)體育系;山西醫(yī)科大學(xué)第一附屬醫(yī)院;
【基金】：山西省衛(wèi)生廳科研基金項(xiàng)目(2011025)
【分類號(hào)】：R87

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本文編號(hào)：2159275