【摘要】：目的:探討耐力運(yùn)動、NAD合成酶相關(guān)基因CG9940以及耐力運(yùn)動聯(lián)合CG9940基因過表達(dá)對中老齡果蠅心臟功能、運(yùn)動能力和壽命的影響。方法:y[1]w[67c23]P{y[+m8]=Mae-UAS.6.11}CG9940[GG01267]雌性果蠅分別與W1118雄性果蠅、arm-Gal4雄性果蠅雜交取F1代分為對照組、運(yùn)動組、過表達(dá)對照組和過表達(dá)運(yùn)動組,4組均收集400只處女蠅,飼養(yǎng)至30天齡開始訓(xùn)練,連續(xù)訓(xùn)練5天,次日取材。實(shí)時熒光定量PCR測定CG9940基因m RNA表達(dá)量,M-mode心動圖檢測心管收縮時間、收縮直徑、舒張直徑、舒張功能不全指數(shù)、射血分?jǐn)?shù)和心律失常指數(shù),測量攀爬指數(shù),統(tǒng)計生命周期。結(jié)果:(1)過表達(dá)對照組CG9940 m RNA過表達(dá)率為155.2%,過表達(dá)運(yùn)動組的過表達(dá)率為152.0%。(2)與對照組相比,運(yùn)動組心率降低(P0.01),射血分?jǐn)?shù)增大(P0.01),舒張功能不全指數(shù)降低(P0.05);過表達(dá)對照組心率降低(P0.05),射血分?jǐn)?shù)增大(P0.01);過表達(dá)運(yùn)動組心率降低(P0.01),心臟平均收縮速度增大(P0.05),射血分?jǐn)?shù)增大(P0.01),舒張功能不全指數(shù)減小(P0.01),心律失常指數(shù)降低(P0.05)。與運(yùn)動組相比,過表達(dá)運(yùn)動組心率降低(P0.05),射血分?jǐn)?shù)增大(P0.01),舒張功能不全指數(shù)減小(P0.05)。與過表達(dá)對照組相比,過表達(dá)運(yùn)動組心率降低(P0.05),射血分?jǐn)?shù)增大(P0.05),舒張功能不全指數(shù)減小(P0.01)。(3)運(yùn)動組攀爬指數(shù)高于對照組(P0.05);過表達(dá)運(yùn)動組攀爬指數(shù)高于對照組(P0.01)和過表達(dá)對照組(P0.05)。(4)運(yùn)動組壽命高于對照組(P0.05);過表達(dá)對照組壽命高于對照組(P0.01);過表達(dá)運(yùn)動組壽命高于對照組(P0.01)、運(yùn)動組(P0.01)和過表達(dá)對照組(P0.05)。結(jié)論:耐力運(yùn)動能夠提高中老年果蠅運(yùn)動能力并且能延緩心臟功能性衰退和延長生命周期;NAD合成酶基因CG9940過表達(dá)能夠延緩果蠅心臟衰老,顯著延長果蠅壽命;耐力運(yùn)動聯(lián)合CG9940基因過表達(dá)能降低中老齡果蠅心律失常和舒張功能不全,同時能增強(qiáng)中老齡果蠅逆重力攀爬特征,提高活動能力,并且延壽效果更好,表明二者對中老齡果蠅抗衰老產(chǎn)生疊了加效應(yīng)。
[Abstract]:Objective: To investigate the effects of endurance exercise, NAD synthase related gene CG9940 and the overexpression of endurance exercise combined with CG9940 gene on the heart function, exercise ability and life span of aged fruit flies. Methods: y[1]w[67c23]P{y[+m8]=Mae-UAS.6.11}CG9940[GG01267] female Drosophila were hybridized with W1118 male Drosophila and arm-Gal4 male Drosophila melanogaster respectively for F1 generation. In the control group, the exercise group, the over expression control group and the overexpressed exercise group, 400 maiden flies were collected in the 4 groups, and the 4 groups were trained to start training at 30 days of age. After 5 days of continuous training, the following day was taken. Real-time fluorescence quantitative PCR was used to determine the expression of CG9940 gene m RNA, M-mode cardiogram was used to detect the systolic time between the heart and tube, the diameter of systole, diastole diameter, diastolic dysfunction index, and the index of diastolic dysfunction. Blood fraction and arrhythmia index, measured climbing index, and statistical life cycle. Results: (1) over expression control group CG9940 m RNA overexpression rate was 155.2%, overexpression of exercise group over expression rate of 152.0%. (2), compared with control group, heart rate decreased (P0.01), ejection fraction (P0.01), diastolic dysfunction index (P0.05); overexpression. In the control group, the heart rate decreased (P0.05) and the ejection fraction increased (P0.01); the heart rate decreased (P0.01) in the overexpressed exercise group, the average systolic velocity of the heart increased (P0.05), the ejection fraction increased (P0.01), the diastolic dysfunction index decreased (P0.01), and the arrhythmia index decreased (P0.05). Compared with the exercise group, the heart rate decreased (P0.05) and the ejection fraction increased. P0.01, diastolic dysfunction index decreased (P0.05). Compared with the overexpressed control group, the heart rate decreased (P0.05), the ejection fraction increased (P0.05), the diastolic dysfunction index decreased (P0.01). (3) the climbing index of the exercise group was higher than that of the control group (P0.05); the climbing index of overexpressed exercise group was higher than that of the control group (P0.01) and the overexpressed control group. (P0.05) (4) the life span of the exercise group was higher than that of the control group (P0.05), and the life expectancy of the overexpressed control group was higher than that of the control group (P0.01); the overexpression of the exercise group was higher than the control group (P0.01), the exercise group (P0.01) and the overexpressed control group (P0.05). Conclusion: endurance exercise can improve the exercise ability of the middle and old aged fruit flies and delay the functional decline of the heart and prolong life. The over expression of NAD synthase gene CG9940 can delay the heart senescence of Drosophila melanogaster and prolong the life of Drosophila melanogaster, and endurance exercise combined with CG9940 gene overexpression can reduce the arrhythmia and diastolic dysfunction of the aged fruit flies, enhance the anti gravity climbing characteristics of the aged fruit flies, improve the activity ability, and prolong the life effect, and show that two It has a stacking effect on the aging of Drosophila melanogaster.
【作者單位】： 湖南師范大學(xué)體適能與運(yùn)動康復(fù)湖南省重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金(31071039) 教育部博士點(diǎn)基金(20134306110009)
【分類號】：R87

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