牽張訓(xùn)練對腓腸肌拉傷大鼠損傷修復(fù)的影響研究
發(fā)布時間：2018-07-15 10:12
【摘要】：實驗?zāi)康?1、通過對腓腸肌急性拉傷后不同時間點組織切片的觀察,明確腓腸肌拉傷后組織結(jié)構(gòu)的修復(fù)過程。2、通過觀察牽張、牽張+冰敷干預(yù)后腓腸肌急性拉傷后不同時間點組織切片的修復(fù)過程,明確腓腸肌損傷后的科學(xué)處理方法。3、通過觀察牽張與牽張+冰敷治療對損傷骨骼肌COL-I蛋白表達(dá)的影響,進(jìn)一步探討牽張與牽張+冰敷對腓腸肌急性拉傷后的作用效果及機理。實驗方法:選取雄性SD大鼠64只,隨機分為正常大鼠組A組、損傷即刻B組、自然恢復(fù)組C_1組、自然恢復(fù)組C_2組、牽張組D_1組、牽張組D_2組、牽張+冰敷組E_1組、牽張+冰敷組E_2組。除正常組外,其余56只大鼠均建立腓腸肌拉傷模型。實驗?zāi)Ｐ椭谱魍瓿珊?D_1組、D_2組在模型建立12天后開始采用牽張手法進(jìn)行康復(fù),每天固定時間、人員操作。E_1組、E_2組在實驗?zāi)Ｐ徒?2天后采用牽張手法康復(fù),然后進(jìn)行冰敷,每天固定時間、人員操作。其余組正常喂養(yǎng)。實驗各組在相應(yīng)的時間進(jìn)行腓腸肌取材,并制作石蠟切片觀察并測試相關(guān)指標(biāo)。實驗結(jié)果:1、大鼠腓腸肌光鏡觀察結(jié)果:拉傷一小時,在光鏡切片下觀察,受損部位肌纖維組織結(jié)構(gòu)不整齊,同時伴隨出現(xiàn)水腫的情況,肌纖維之間的間隙情況不一致,有的明顯加大,可觀察到存在炎癥反應(yīng),并可見血管擴(kuò)張發(fā)生。在骨骼肌受損后18天組織切片觀察可見,自然恢復(fù)組受損部位肌纖維出現(xiàn)修復(fù),但沒有完全修復(fù),肌纖維存在大小不一,排列不夠整齊。牽張組與牽張+冰敷組治療后,受損部位新生的肌纖維排列更加整齊,結(jié)構(gòu)更加完整。在骨骼肌受損后25天光鏡觀察可見,自然恢復(fù)組受損部位肌纖維出現(xiàn)修復(fù),修復(fù)存在瘢痕,肌纖維存在大小不一,排列不夠整齊。牽張組與牽張+冰敷組治療后,受損部位新生的肌纖維基本排列整齊,修復(fù)較好。2、大鼠腓腸肌膠原蛋白COL-I免疫組化測試結(jié)果:損傷一小時,COL-I蛋白表達(dá)隨即下降,在損傷18天自然恢復(fù)組C1、牽張組D_1、牽張+冰敷組E_1膠原蛋白COL-I蛋白表達(dá)均出現(xiàn)最大值,自然恢復(fù)組B_1組與正常組存在極顯著性差異(P0.01),與損傷即刻組B組有極顯著性差異(P0.01),牽張組D_1組、牽張+冰敷組E_1組與損傷即刻B組、正常組COL-I蛋白表達(dá)均存在顯著性差異(P0.05)。在損傷25天自然恢復(fù)組C_2組與正常組存在顯著性差異(P0.05),牽張組D_2組、牽張+冰敷組E_2組與正常組COL-I蛋白表達(dá)均無顯著性差異。牽張組D_1牽張+冰敷組E_1無顯著性差異,牽張組D_2牽張+冰敷組E_2無顯著性差異。牽張組D_1與牽張組D_2存在顯著性差異(P0.05),牽張+冰敷組E_1與、牽張+冰敷組E_2存在顯著性差異(P0.05)。實驗結(jié)論:1、腓腸肌在發(fā)生損傷后,采用牽張、牽張+冰敷治療,有利于損傷后骨骼肌的康復(fù),具有促進(jìn)愈合的效果。2、腓腸肌急性拉傷后采用牽張與牽張+冰敷治療,腓腸肌拉傷修復(fù)過程中瘢痕組織的生成減少。3、腓腸肌修復(fù)過程中,牽張訓(xùn)練在后期治療更有利于骨骼肌修復(fù)。
[Abstract]:Objective to investigate the repair process of gastrocnemius muscle after acute stretch injury by observing tissue sections at different time points after gastrocnemius injury. The repair process of tissue sections of gastrocnemius muscle at different time points after acute stretch injury after the intervention of stretch ice compress was studied, and the scientific treatment method of gastrocnemius muscle injury was determined. The effect of stretch and stretch ice compress therapy on the expression of COL-I protein in injured skeletal muscle was observed by observing the effect of stretch and stretch ice compress therapy on the expression of COL-I protein in injured skeletal muscle. To investigate the effect and mechanism of stretch and stretch ice compress on gastrocnemius muscle after acute injury. Methods: 64 male Sprague-Dawley rats were randomly divided into normal group A, immediate injury group B, natural recovery group C1, natural recovery group C 2, distraction group D 1, distraction group D 2, distraction ice compress group E _ 1. Stretch ice compress group Es 2 group. Gastrocnemius muscle injury model was established in 56 rats except normal group. After the establishment of the experimental model, D1 group and D2 group began to recover with distraction technique 12 days after the establishment of the model. The daily fixed time was fixed. After 12 days after the establishment of the experimental model, the two groups were rehabilitated by distraction technique after the establishment of the experimental model, and then ice compress was carried out. Daily fixed time, personnel operation. The rest group was fed normally. The samples of gastrocnemius muscle were taken at the corresponding time, and paraffin sections were made to observe and test the related indexes. The results of the experiment were as follows: 1. The results of the observation of gastrocnemius muscle light microscope in rats were as follows: after one hour of tensile injury, the tissue structure of muscle fibers in the damaged area was irregular, accompanied by edema, and the gap between muscle fibers was not consistent. Some obviously enlarged, the existence of inflammation can be observed, and vasodilation can be seen. On the 18th day after skeletal muscle injury, it was observed that the muscle fibers were repaired in the injured part of the natural recovery group, but not completely repaired. The size of the muscle fibers was different and the arrangement was not neat. After treatment, the new muscle fibers in the injured areas were more neatly arranged and more complete in structure after treatment in the distraction group and the stretch ice group. After 25 days of skeletal muscle injury, it can be seen that the muscle fibers in the natural recovery group were repaired, scar was repaired, the size of muscle fiber was different, and the arrangement was not neat. After treatment, the neonate muscle fibers in the injured areas were arranged neatly and repaired well. The results of immunohistochemical staining of collagen COL-I in gastrocnemius muscle of rats showed that the expression of COL-I protein decreased immediately after one hour of injury. On the 18th day after injury, the expression of collagen COL-I protein in the natural recovery group, D1 group and ice compress group showed the maximum expression. There was significant difference between group B1 and normal group (P0.01), and between group B and group B immediately after injury (P0.01). The expression of COL-I protein in group D1, group E1, group E1 and group B immediately after injury in distraction group was significantly different from that in group B (P0.01). There was a significant difference in the expression of COL-I protein in normal group (P0.05). There was no significant difference in COL-I protein expression between the two groups and the normal group on the 25th day after injury (P0.05). There was no significant difference in the expression of COL-I protein between the two groups (P 0.05), the distraction group (D2), the stretch ice compress group (E2 group) and the normal group (P 0.05). There was no significant difference in E _ 1 between D _ 1 and D _ 1 stretch ice compress group, but no significant difference in E _ (2) in D _ 2 stretch ice compress group. There was significant difference between D1 and D2 in distraction group (P0.05), significant difference in ESP 1 and E2 in distraction ice compress group (P0.05). Conclusion: 1. After injury, the gastrocnemius muscle was treated with stretch and stretch ice compress, which was beneficial to the recovery of skeletal muscle after injury, and had the effect of promoting healing. The gastrocnemius muscle was treated with stretch and stretch ice compress after acute injury of gastrocnemius. During the repair of gastrocnemius, the scar tissue formation decreased by 0.3. During the repair of gastrocnemius muscle, stretch training was more beneficial to skeletal muscle repair.
【學(xué)位授予單位】：河南師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R87
本文編號：2123730
[Abstract]:Objective to investigate the repair process of gastrocnemius muscle after acute stretch injury by observing tissue sections at different time points after gastrocnemius injury. The repair process of tissue sections of gastrocnemius muscle at different time points after acute stretch injury after the intervention of stretch ice compress was studied, and the scientific treatment method of gastrocnemius muscle injury was determined. The effect of stretch and stretch ice compress therapy on the expression of COL-I protein in injured skeletal muscle was observed by observing the effect of stretch and stretch ice compress therapy on the expression of COL-I protein in injured skeletal muscle. To investigate the effect and mechanism of stretch and stretch ice compress on gastrocnemius muscle after acute injury. Methods: 64 male Sprague-Dawley rats were randomly divided into normal group A, immediate injury group B, natural recovery group C1, natural recovery group C 2, distraction group D 1, distraction group D 2, distraction ice compress group E _ 1. Stretch ice compress group Es 2 group. Gastrocnemius muscle injury model was established in 56 rats except normal group. After the establishment of the experimental model, D1 group and D2 group began to recover with distraction technique 12 days after the establishment of the model. The daily fixed time was fixed. After 12 days after the establishment of the experimental model, the two groups were rehabilitated by distraction technique after the establishment of the experimental model, and then ice compress was carried out. Daily fixed time, personnel operation. The rest group was fed normally. The samples of gastrocnemius muscle were taken at the corresponding time, and paraffin sections were made to observe and test the related indexes. The results of the experiment were as follows: 1. The results of the observation of gastrocnemius muscle light microscope in rats were as follows: after one hour of tensile injury, the tissue structure of muscle fibers in the damaged area was irregular, accompanied by edema, and the gap between muscle fibers was not consistent. Some obviously enlarged, the existence of inflammation can be observed, and vasodilation can be seen. On the 18th day after skeletal muscle injury, it was observed that the muscle fibers were repaired in the injured part of the natural recovery group, but not completely repaired. The size of the muscle fibers was different and the arrangement was not neat. After treatment, the new muscle fibers in the injured areas were more neatly arranged and more complete in structure after treatment in the distraction group and the stretch ice group. After 25 days of skeletal muscle injury, it can be seen that the muscle fibers in the natural recovery group were repaired, scar was repaired, the size of muscle fiber was different, and the arrangement was not neat. After treatment, the neonate muscle fibers in the injured areas were arranged neatly and repaired well. The results of immunohistochemical staining of collagen COL-I in gastrocnemius muscle of rats showed that the expression of COL-I protein decreased immediately after one hour of injury. On the 18th day after injury, the expression of collagen COL-I protein in the natural recovery group, D1 group and ice compress group showed the maximum expression. There was significant difference between group B1 and normal group (P0.01), and between group B and group B immediately after injury (P0.01). The expression of COL-I protein in group D1, group E1, group E1 and group B immediately after injury in distraction group was significantly different from that in group B (P0.01). There was a significant difference in the expression of COL-I protein in normal group (P0.05). There was no significant difference in COL-I protein expression between the two groups and the normal group on the 25th day after injury (P0.05). There was no significant difference in the expression of COL-I protein between the two groups (P 0.05), the distraction group (D2), the stretch ice compress group (E2 group) and the normal group (P 0.05). There was no significant difference in E _ 1 between D _ 1 and D _ 1 stretch ice compress group, but no significant difference in E _ (2) in D _ 2 stretch ice compress group. There was significant difference between D1 and D2 in distraction group (P0.05), significant difference in ESP 1 and E2 in distraction ice compress group (P0.05). Conclusion: 1. After injury, the gastrocnemius muscle was treated with stretch and stretch ice compress, which was beneficial to the recovery of skeletal muscle after injury, and had the effect of promoting healing. The gastrocnemius muscle was treated with stretch and stretch ice compress after acute injury of gastrocnemius. During the repair of gastrocnemius, the scar tissue formation decreased by 0.3. During the repair of gastrocnemius muscle, stretch training was more beneficial to skeletal muscle repair.
【學(xué)位授予單位】：河南師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R87
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