本文選題：活性肽 + Calpain�。� 參考：《北京體育大學(xué)》2012年碩士論文

【摘要】：1研究目的本實驗通過觀察補充活性肽對Calpain-1.Calpain-2基因表達的影響來探討活性肽在預(yù)防骨骼肌蛋白降解中所發(fā)揮的作用,另外還期望在亞細胞水平對骨骼肌微損傷的生理調(diào)節(jié)機制進行一定程度的闡釋 2研究方法8周齡雄性SD大鼠隨機分為安靜對照組,運動后即刻、24h、48h對照組,運動后即刻、24h、48h+海洋肽實驗組,運動后即刻、24h、48h+大豆蛋白陽性對照組,共10組,每組10只,進行6周跑臺訓(xùn)練。采用放射免疫法測定血清T.IGF-1含量；采用Real Time-PCR法測定骨骼肌Calpain-1.Calpain-2mRNA含量。 3研究結(jié)果3.1運動后,海洋肽運動組血清IGF-1與單純運動組、大豆蛋白運動組無顯著性差異；隨時間變化,運動后即刻與運動前差異顯著(P0.05)。3.2運動后24h,海洋肽運動組血清睪酮與單純運動組差異顯著(P0.05)；隨時間變化無顯著性差異。3.3海洋肽運動組運動后即刻股中肌CANP-1基因表達變化與單純運動組、大豆蛋白運動組差異顯著(P0.05)；隨時間變化,差異非常顯著(P0.05)。運動后24h,股中肌CANP-2基因表達變化與大豆蛋白運動組差異顯著(P0.05)；隨時間變化,無顯著性差異。3.4海洋肽運動組運動后24h,腓腸肌CANP-1基因表達變化與單純運動組差異顯著(P0.05)；運動后48h,與大豆蛋白運動組差異顯著(P0.01)；隨時間變化,無顯著性差異。運動后即刻、運動后48h,腓腸肌CANP-2基因表達變化與大豆蛋白運動組差異顯著(P0.01);隨時間變化,差異非常顯著(P0.01)。 4研究結(jié)論4.1海洋肽與大豆蛋白均能夠縮短運動后血清IGF-1、血清T的恢復(fù)時間；海洋肽的效果好于大豆蛋白,促進睪酮恢復(fù)的速率更快。4.2運動后,骨骼肌CANP-1、CANP-2基因表達呈現(xiàn)不同變化；CANP-1基因表達隨時間延長持續(xù)下調(diào),而CANP-2基因表達上調(diào)。4.3海洋肽在運動過程中對骨骼肌有一定的保護作用,而大豆蛋白主要在運動后48h的炎癥反應(yīng)階段發(fā)揮保護作用。4.4運動后股中肌CANP-1基因表達持續(xù)下調(diào),而腓腸肌CANP-1.CANP-2基因表達呈現(xiàn)為先增高后降低,這與肌纖維類型有關(guān)。
[Abstract]:Objective to investigate the role of active peptides in the prevention of skeletal muscle protein degradation by observing the effect of supplementation of active peptides on the expression of Calpain-1.Calpain-2 gene. In addition, it is expected to explain to a certain extent the physiological regulation mechanism of skeletal muscle microdamage at the subcellular level. Methods the 8-week-old male SD rats were randomly divided into quiet control group and control group for 24 h and 48 h immediately after exercise. Immediately after exercise, 10 rats in 10 groups were treated with marine peptide for 24 hours and 48 hours after exercise, and the control group with soybean protein positive for 24 hours and 48 hours after exercise. 10 rats in each group were trained by running platform for 6 weeks. The levels of serum T.IGF-1 and Calpain-1.Calpain-2 mRNA in skeletal muscle were measured by radioimmunoassay and Real Time-PCR respectively. 3 the results showed that there was no significant difference in serum IGF-1 between marine peptide exercise group and soybean protein exercise group after 3.1 exercise. There were significant differences in serum testosterone between exercise group and exercise group (P0.05) immediately after exercise and 24 hours after exercise (P0.05). There was no significant difference with time. 3.3 the changes of CANP-1 gene expression in middle femoral muscle immediately after exercise in marine peptide exercise group were significantly different from those in simple exercise group and soybean protein exercise group (P0.05), and the difference was very significant with time (P0.05). The changes of CANP-2 gene expression in middle femoris muscle were significantly different from those in soybean protein exercise group at 24 h after exercise (P0.05), but there was no significant difference between exercise group and exercise group at 24 h after exercise (P0.05), and there was no significant difference in CANP-1 gene expression in gastrocnemius muscle between exercise group and exercise group 24 hours after exercise (P0.05). There was a significant difference between the exercise group and the soybean protein group 48 hours after exercise (P0.01), but there was no significant difference with time. The changes of CANP-2 gene expression in gastrocnemius muscle immediately after exercise and 48 hours after exercise were significantly different from those in soybean protein exercise group (P0.01). The difference was very significant (P0.01). 4 conclusion 4.1 Marine peptide and soybean protein can shorten the recovery time of serum IGF-1 and serum T after exercise, the effect of marine peptide is better than that of soybean protein, and the recovery rate of testosterone is faster after exercise .4.2. The expression of CANP-1 and CANP-2 in skeletal muscle showed different changes. CANP-1 gene expression continued to be down-regulated over time, while CANP-2 gene expression up-regulated .4.3 marine peptide had protective effect on skeletal muscle during exercise. The expression of CANP-1 gene in femur muscle decreased continuously after exercise 48 h after exercise, while CANP-1. CANP-2 gene expression in gastrocnemius muscle increased first and then decreased, which was related to the type of muscle fiber.
【學(xué)位授予單位】：北京體育大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：G804.2

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