發(fā)布時(shí)間：2018-06-25 16:24

  本文選題：法醫(yī)物證學(xué) + 等位基因分型標(biāo)準(zhǔn)物�。� 參考：《山西醫(yī)科大學(xué)》2013年碩士論文

【摘要】：目的 調(diào)查D11S4463基因座和D17S974基因座在中國(guó)漢族人群中的遺傳多態(tài)性,并用分子克隆技術(shù)制備其等位基因分型標(biāo)準(zhǔn)物。 方法 用熒光PCR和ABI3130XL遺傳分析儀對(duì)中國(guó)漢族600份無(wú)關(guān)個(gè)體血斑樣本進(jìn)行D11S4463和D17S974基因座遺傳多態(tài)性調(diào)查,并用分子克隆的方法構(gòu)建其等位基因分型標(biāo)準(zhǔn)物。首先在人群中分別篩選出D11S4463基因座的9個(gè)等位基因和D17S974基因座的8個(gè)等位基因,經(jīng)PCR擴(kuò)增,并將純化后的擴(kuò)增產(chǎn)物與pMD18-T質(zhì)粒載體連接后轉(zhuǎn)化到DH5α感受態(tài)大腸桿菌中；篩選重組子及提取重組質(zhì)粒,對(duì)等位基因重組質(zhì)粒進(jìn)行序列測(cè)定,并對(duì)等位基因進(jìn)行標(biāo)準(zhǔn)命名；以等位基因重組質(zhì)粒為模板制備等位基因分型標(biāo)準(zhǔn)物。 結(jié)果 D11S4463基因座和D17S974基因座在中國(guó)漢族人群中具有較高的遺傳多態(tài)性,分別檢測(cè)出9個(gè)和8個(gè)等位基因,其觀察雜合度分別為0.735和0.747；匹配概率分別為0.089和0.115；個(gè)體識(shí)別能力分別為0.911和0.885；多態(tài)性信息含量分別為0.73和0.70；非父排除概率分別為0.485和0.505。應(yīng)用分子克隆方法成功構(gòu)建了D11S4463基因座和D17S974基因座等位基因分型標(biāo)準(zhǔn)物。 結(jié)論 D11S4463基因座和D17S974基因座具有較高的遺傳多態(tài)性,是一個(gè)適合于法醫(yī)學(xué)和群體遺傳學(xué)研究的遺傳標(biāo)記,應(yīng)用分子克隆的方法構(gòu)建其等位基因分型標(biāo)準(zhǔn)物是一種實(shí)現(xiàn)其等位基因分型標(biāo)準(zhǔn)化的可行辦法。
[Abstract]:Objective to investigate the genetic polymorphism of D11S4463 locus and D17S974 locus in Chinese Han population and to prepare their alleles by molecular cloning. Methods the genetic polymorphisms of D11S4463 and D17S974 loci were investigated by fluorescence PCR and ABI3130XL genetic analyzer in 600 unrelated individuals of Han nationality in China, and their alleles were constructed by molecular cloning. At first, 9 alleles of D11S4463 locus and 8 alleles of D17S974 locus were screened out in the population. The purified products were amplified by PCR and transformed into DH5 偽 competent Escherichia coli by ligation with pMD18-T plasmid vector. The allelic recombinant plasmids were sequenced and the alleles were named as standard alleles, and the allelic genotyping standard compounds were prepared by using allelic recombinant plasmids as template. Results D11S4463 locus and D17S974 locus were highly polymorphic in Chinese Han population, 9 and 8 alleles were detected respectively, the observed heterozygosity were 0.735 and 0.747, the matching probability were 0.089 and 0.115, respectively. The recognition ability of individuals was 0.911 and 0.885, the polymorphic information content was 0.73 and 0.70, and the non-paternal exclusion probability was 0.485 and 0.505, respectively. The alleles of D11S4463 locus and D17S974 locus were successfully constructed by molecular cloning. Conclusion D11S4463 locus and D17S974 locus are highly polymorphic, which is a suitable genetic marker for forensic and population genetics research. It is a feasible method to construct its allelic genotyping standard by molecular cloning.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：D919.4

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本文編號(hào)：2066708