發(fā)布時(shí)間：2018-06-21 11:34

  本文選題：帕金森癥 + 行為學(xué)測試�。� 參考：《揚(yáng)州大學(xué)》2014年碩士論文

【摘要】：目的： 在成功建立小鼠慢性帕金森模型的基礎(chǔ)上,研究運(yùn)動對帕金森癥小鼠在行為學(xué)上的促進(jìn)作用,探究運(yùn)動對小鼠腦部谷胱甘肽、以及神經(jīng)營養(yǎng)因子的干預(yù)作用。 方法： 選用清潔級雄性C57BL/6J小鼠,8周齡40只,體重24+3g,常規(guī)條件喂養(yǎng),隨機(jī)將小鼠分為5組,分別為：健康安靜組(A組,n=8)、MPTP注射組(B組,n=8)、健康運(yùn)動組(C組,n=8)、運(yùn)動+MPTP注射組(D組,n=8)、MPTP注射+運(yùn)動組(E組,n=8),對實(shí)驗(yàn)組小鼠注射MPTP,建立小鼠慢性帕金森癥模型。各組運(yùn)動組小鼠每天進(jìn)行中等強(qiáng)度轉(zhuǎn)輪訓(xùn)練(20轉(zhuǎn)/份),每次30分鐘,連續(xù)4周。對各階段小鼠進(jìn)行行為學(xué)測試及體重測定,取材前12小時(shí)禁食水,并對小鼠進(jìn)行體重、行為學(xué)檢測。最后一次運(yùn)動刺激后進(jìn)行取材,檢測各組小鼠腦部GSH含量、GSH-PX活性、BDNF、GDNF蛋白表達(dá)水平等指標(biāo)。 結(jié)果： 1.通過對小鼠腹腔注射MPTP (溶于9g/L生理鹽水中,終濃度為1g、L),每周2次,間隔3天,每次15mg/kg,連續(xù)4周成功建立慢性帕金森癥小鼠模型。 2.運(yùn)動組行為學(xué)測試成績與安靜組相比差異顯著(P0.01),D、E兩組與B組相比較有顯著性差異(P0.01)。而D組與E組相比,有運(yùn)動基礎(chǔ)的D組小鼠行為學(xué)測試的數(shù)據(jù)稍好于E組小鼠(P0.05)。 3.MPTP組動物腦內(nèi)GSH含量明顯減少、GSH-PX活性明顯降低,安靜健康組小鼠與安靜運(yùn)動組小鼠在GSH含量及GSH-PX活性上都存在顯著性差異(P0.01),而D組小鼠與E組小鼠在兩者比較中都顯示出一定差異(P0.05),并體現(xiàn)出一定趨勢。 4.B組與D、E組小鼠蛋白表達(dá)存在差異(P0.01),顯示運(yùn)動可以增加帕金森小鼠腦內(nèi)BDNF、GDNF的蛋白表達(dá),同時(shí)D組小鼠與E組小鼠腦部BDNF、GDNF蛋白相對含量也存在差異(P0.05),顯示有長期運(yùn)動基礎(chǔ)及運(yùn)動習(xí)慣的小鼠,腦內(nèi)BDNF、GDNF蛋白含量高于康復(fù)運(yùn)動組小鼠。 結(jié)論： 1.有氧運(yùn)動可改善帕金森癥小鼠行為學(xué)表現(xiàn)及其肢體運(yùn)動功能。 2.有氧運(yùn)動的練習(xí),對PD小鼠腦內(nèi)GSH含量的提高,GSH-PX活性的增強(qiáng)有顯著的作用,使對神經(jīng)細(xì)胞有害的羥自由基受到抑制,神經(jīng)細(xì)胞得到保護(hù)或恢復(fù)。 3.通過運(yùn)動可提高PD小鼠腦內(nèi)對神經(jīng)元具有顯著保護(hù)和促進(jìn)修復(fù)作用的BDNF、GDNF蛋白表達(dá)水平,揭示運(yùn)動對多巴胺能神經(jīng)元的保護(hù)和修復(fù)作用。
[Abstract]:Aim: on the basis of the successful establishment of chronic Parkinson's model in mice, the effects of exercise on the behavior of mice with Parkinson's disease were studied, and the effects of exercise on glutathione and neurotrophic factor in brain of mice were investigated. Methods: 40 C57BL / 6J C57BL / 6J C57BL / 6J C57BL / 6J mice were randomly divided into 5 groups. The rats in the healthy and quiet group were injected with MPTP in group A, group B in group B, group C in healthy exercise group, group D in group D and group E, respectively. The model of chronic Parkinson's disease was established by injection of MPTPin the experimental group. The mice in the exercise group were treated with moderate intensity wheel training for 20 cycles per day for 30 minutes for 4 weeks. Behavioral test and body weight measurement were carried out in each stage of mice, and fasting water was taken 12 hours before sampling, and the body weight and behavior of mice were measured. The GSH content and GSH-PX activity of the brain and the expression level of BDNF GDNF protein were measured after the last exercise stimulation. Results: 1. A mouse model of chronic Parkinson's disease was successfully established by intraperitoneal injection of MPTP (dissolved in 9 g / L normal saline with a final concentration of 1 g / L) twice a week for 3 days at a interval of 15 mg / kg for 4 weeks. The scores of behavioral test in exercise group were significantly different from those in quiet group (P 0.01). There was significant difference between two groups (P 0.01) and group B (P 0.01). Compared with group E, the behavioral test data of group D with exercise basis were slightly better than that of group E (P 0.05). 3. The activity of GSH-PX in the brain of rats in MPTP group was significantly reduced, and the activity of GSH-PX was significantly decreased in group D than that in group E, and the activity of GSH-PX in group D was significantly lower than that in group E. There was a significant difference in GSH content and GSH-PX activity between the healthy group and the rest exercise group, while there was a certain difference between group D and E mice in the content of GSH and the activity of GSH-PX, and showed a certain trend. 4. There was a difference in protein expression between group B and group DNE, indicating that exercise could increase the expression of BDNF GDNF in the brain of Parkinson's mice. At the same time, the relative content of BDNF GDNF protein in the brain of group D and E mice was also different (P 0.05), which showed that the content of BDNFN GDNF protein in brain of group D mice was higher than that of mice with rehabilitation exercise group. Conclusion: 1. Aerobic exercise can improve the behavior of Parkinson's disease mice and limb motor function. 2. The exercise of aerobic exercise had a significant effect on the increase of GSH content in brain of PD mice and the increase of GSH-PX activity, which inhibited the harmful hydroxyl radicals and protected or restored the nerve cells. Exercise can improve the expression level of BDNF GDNF protein which can significantly protect and promote the repair of neurons in the brain of PD mice, and reveal the protective and repair effects of exercise on dopaminergic neurons.
【學(xué)位授予單位】：揚(yáng)州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：G804.2;R87

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