本文選題：間歇性禁食 + 骨骼肌質量�。� 參考：《中國運動醫(yī)學雜志》2017年08期

【摘要】：目的:探討4周間歇性禁食后大鼠體重、體脂及骨骼肌質量的變化與大鼠骨骼肌自噬的關系,為間歇性禁食提供理論依據(jù)。方法:20只雄性SD大鼠隨機分為對照組(Con組)和間歇性禁食組(IF組),每組各10只。采取每周三、周五間歇性禁食方案,每周按時記錄大鼠體重,4周干預后雙能X線吸收測量法(DEXA)分析體脂含量,之后分離雙側比目魚肌稱量濕重,免疫熒光法檢測比目魚肌laminin蛋白體現(xiàn)肌肉橫截面積,透射電鏡觀察比目魚肌自噬泡形態(tài),Western Blot檢測自噬相關蛋白LC3、p62及調控蛋白AMPKα、p-AMPKα、ULK1的表達情況。結果:4周間歇性禁食后,間歇性禁食組大鼠體重與體脂含量顯著低于對照組(P0.01),但各組比目魚肌濕重及肌纖維橫截面積并無明顯差異(P0.05)。間歇性禁食組比目魚肌AMPKα、p-AMPKα、ULK1蛋白表達水平明顯高于對照組(P0.01),間歇性禁食組比目魚肌內自噬標記LC3-Ⅱ/LC3-Ⅰ水平明顯高于對照組,p62表達水平明顯低于對照組,均具有非常顯著性差異(P0.01)。結論:4周間歇性禁食明顯降低體脂水平,具有控制體重的作用,可激活AMPK-ULK1通路適度促進骨骼肌自噬,以維持骨骼肌質量,可作為一種潛在的"減肥"方法進行深入研究。
[Abstract]:Objective: to investigate the relationship between the changes of body weight, body fat and skeletal muscle mass and the autophagy of skeletal muscle in rats after intermittent fasting for 4 weeks, and to provide a theoretical basis for intermittent fasting. Methods Twenty male Sprague-Dawley rats were randomly divided into control group (Con group) and intermittent fasting group (10 rats in each group). The rats' body fat content was measured by dual energy X-ray absorptiometry after 4 weeks of intervention, and the wet weight of bilateral soleus muscle was separated. The cross sectional area of soleus muscle laminin protein was detected by immunofluorescence, and the expression of autophagy associated protein LC3p62 and the regulatory protein AMPK 偽 p-AMPK 偽 mULK1 in soleus muscle were observed by transmission electron microscope (TEM). Results after 4 weeks of intermittent fasting, the body weight and body fat content of rats in the intermittent fasting group were significantly lower than those in the control group (P 0.01), but there was no significant difference in wet weight of soleus muscle and cross sectional area of muscle fiber in each group. The expression of AMPK 偽 -p-AMPK 偽 -UULK1 protein in the soleus muscle of intermittent fasting group was significantly higher than that in the control group (P 0.01), and the expression level of LC- 鈪，

本文編號：2007488