本文選題：琥珀酸脫氫酶 + 丙酮酸激酶��； 參考：《遼寧師范大學(xué)》2012年碩士論文

【摘要】：研究目的：通過選取8周齡雄性KM小鼠為研究材料，設(shè)計(jì)不同強(qiáng)度的跑臺訓(xùn)練和海參小分子肽補(bǔ)充的組合方案，檢測訓(xùn)練結(jié)束后小鼠骨骼肌中丙酮酸激酶（PK）、蘋果酸脫氫酶(MDH)和琥珀酸脫氫酶(SDH)活性，記錄各組小鼠訓(xùn)練完成后游泳力竭的時(shí)間，通過探討不同運(yùn)動形式和營養(yǎng)補(bǔ)充狀態(tài)下能量供應(yīng)系統(tǒng)代謝酶的變化特點(diǎn)和規(guī)律，揭示長期跑臺訓(xùn)練對小鼠骨骼肌能量代謝酶的影響，為發(fā)展無氧和有氧能力提供一定的參考。最后探討海參小分子肽對機(jī)體抗疲勞能力的影響，為運(yùn)動營養(yǎng)品的開發(fā)提供參考。 研究方法：將30只雄性KM小鼠隨機(jī)分成對照組（C）、有氧運(yùn)動組（T1）、無氧運(yùn)動組（T2）、有氧運(yùn)動營養(yǎng)補(bǔ)充組（T3）、無氧運(yùn)動營養(yǎng)補(bǔ)充組（T4），每組6只。以電動動物跑臺作為訓(xùn)練器材，首先將T1、T2、T3、T4組常規(guī)飼養(yǎng)3天，而后進(jìn)行1周的適應(yīng)性訓(xùn)練，速度為8m/min，10min/d，適應(yīng)訓(xùn)練完成后，T1、T3組小鼠進(jìn)行為期6周76%VO2max的有氧跑臺訓(xùn)練，T2、T4組小鼠進(jìn)行95%VO2max的無氧跑臺訓(xùn)練，訓(xùn)練強(qiáng)度為極限強(qiáng)度，每周訓(xùn)練6天，跑臺坡度為00。各組小鼠在訓(xùn)練結(jié)束后（6w）從眼靜脈取血0.5～1.5ml，靜脈血以2500rpm離心10分鐘，取上清液置于4°C冰箱中保存，用于測定小鼠血清SOD、MDA含量。取靜脈血完成后斷頸處死，同時(shí)迅速取右腿股四頭肌清洗、除去血液、濾紙拭干、稱重、放入小燒杯內(nèi)。用研磨器在冰浴的條件下充分研碎,使組織成為勻漿。將制備好的10%組織勻漿用低溫離心機(jī)以2500rpm速度離心10min,取上清放于樣品管中-70℃冷藏備用，上清液用于各種指標(biāo)的測定。采用酶免法測小鼠股四頭肌中MDH、SDH、PK活性。將各組數(shù)據(jù)進(jìn)行統(tǒng)計(jì)處理，進(jìn)行比較和分析。 實(shí)驗(yàn)結(jié)果： （1）經(jīng)過6周的跑臺訓(xùn)練和營養(yǎng)補(bǔ)充后，無氧訓(xùn)練組小鼠股四頭肌中PK活性有所增加，與對照組相比，，T2組有差異顯著（P0.05），T4組差異非常顯著（P0.01），T4與T2組相比差異顯著（P0.05）；T3與T1相比較無顯著變化。 （2）經(jīng)過6周的跑臺訓(xùn)練和營養(yǎng)補(bǔ)充，有氧訓(xùn)練組小鼠股四頭肌中MDH、SDH活性有所增加，與對照組相比，T3組有非常顯著性差異（P0.01），T1組有顯著性變化（P0.05），T3與T1組相比較變化顯著（P0.05）；T4與T2相比較無顯著性變化。 （3）經(jīng)過6周的跑臺訓(xùn)練和營養(yǎng)補(bǔ)充,有氧訓(xùn)練組小鼠游泳力竭時(shí)間與對照組小鼠相比較變化顯著（P0.01）；無氧訓(xùn)練組小鼠游泳力竭時(shí)間與對照組相比有所增加，T4變化顯著（P0.05），T2無顯著性變化，無統(tǒng)計(jì)學(xué)意義；T3組與T1組相比較發(fā)現(xiàn)有顯著性變化（P0.05），T4組與T2相比較有顯著性差異（P0.05）。 （4）經(jīng)過6周的跑臺訓(xùn)練和營養(yǎng)補(bǔ)充，發(fā)現(xiàn)小鼠血清SOD、MDA發(fā)生不同程度的變化。T1、T2、T4組小鼠血清SOD含量變化非常顯著（P0.01），T2組有顯著性變化（P0.05）。T1、T2、T4組小鼠血清MDA含量變化非常顯著（P0.01），T2組沒有顯著性變化。同運(yùn)動形式組別之間比較有顯著性差異（P0.05）。 結(jié)論：（1）長期的無氧運(yùn)動訓(xùn)練可以使小鼠骨骼肌PK活性增加，表明長期的無氧訓(xùn)練通過調(diào)節(jié)代謝酶的活性，提高糖酵解的能力。 （2）長期有氧訓(xùn)練可以有效地提高小鼠骨骼肌組織的SDH，MDH活性，表明長期有氧訓(xùn)練可以提高身體的有氧供能的能力，增加三羧酸循環(huán)的酶的活性。 （3）長期的有氧訓(xùn)練，有氧代謝活動顯著增加;經(jīng)常在無氧訓(xùn)練無氧代謝酶的活性也相應(yīng)增加。 （4）補(bǔ)充海參的小分子肽，可以提高SDH，MDH，PK的活性，這可能是它被吸收轉(zhuǎn)換成SDH、MDH、PK的原料或者與抗疲勞能力的改善有關(guān)，具體機(jī)制不明確需要進(jìn)一步研究。 （5）補(bǔ)充海參小分子肽，可以提高小鼠的抗疲勞能力。
[Abstract]:Objective: to determine the activity of pyruvate kinase (PK), malate dehydrogenase (MDH) and succinic dehydrogenase (SDH) in skeletal muscles of mice after training, and record the swimming ability of mice after training by selecting 8 weeks male KM mice as the research materials. At the time of exhaustion, the effects of long run platform training on energy metabolism enzymes in skeletal muscles of mice were revealed by exploring the characteristics and rules of metabolic enzymes in the energy supply system under different forms of exercise and nutritional supplement. Finally, the effect of small molecular peptides on the anti fatigue ability of the body was discussed. It provides a reference for the development of sports nutritions.
Study methods: 30 male KM mice were randomly divided into control group (C), aerobic exercise group (T1), anaerobic exercise group (T2), aerobic exercise supplement group (T3), anaerobic exercise supplement group (T4), each group was used as training equipment, and the group of T1, T2, T3, T4 was raised for 3 days, and then 1 weeks of adaptive training, and then the speed of adaptive training, and the speed of 1 weeks. For 8m/min, 10min/d, after the completion of training, T1, T3 group mice were trained for 6 weeks 76%VO2max, T2, T4 group were trained with 95%VO2max without oxygen, training intensity was the ultimate strength, training 6 days a week, and 0 groups of mice at the end of training (6W) from the eye vein, 0.5 ~ 1.5ml, and venous blood for 2500rpm. For 10 minutes centrifugation, the supernatant was stored in the 4 degree C refrigerator and used to determine the serum SOD and MDA content of the mice. After the venous blood was completed, the neck was executed and the four head of the right leg was cleaned quickly. The blood was removed, the filter paper was dried and weighed in a small beaker. The tissue became a homogenate under the condition of the ice bath to make the tissue a homogenate. The tissue would be made to be a homogenate. The 10% tissue homogenate was centrifuged at the speed of 2500rpm with low temperature centrifuge for 10min, and the supernatant was stored in the sample tube at -70 C for cold storage, and the supernatant was used for the determination of various indexes. The activity of MDH, SDH and PK in the four muscles of the mouse femoris was measured by enzyme immunoassay. The data of each group were statistically processed to compare and analyze.
Experimental results:
(1) after 6 weeks of treadmill training and nutrition supplement, the activity of PK in the four head muscles of the mice of the anaerobic training group increased. Compared with the control group, there was significant difference in the T2 group (P0.05), the difference in the T4 group was very significant (P0.01), and the difference between T4 and T2 group was significant (P0.05), and there was no significant change between T3 and T1.
(2) after 6 weeks of training and nutrition supplement, the activity of MDH and SDH increased in the four head muscle of the mice of the aerobic training group. Compared with the control group, there was a significant difference in the T3 group (P0.01), the T1 group had a significant change (P0.05), and the T3 and T1 group had a significant change (P0.05), and there was no significant change in T4 and T2.
(3) after 6 weeks of treadmill training and nutrition supplement, the swimming exhaustion time of the mice in the aerobic training group was significantly higher than that of the control group (P0.01). The time of swimming exhaustion in the anaerobic training group was increased compared with the control group, and the change of T4 was significant (P0.05), and there was no significant change in T2. The T3 group and the T1 group found that there was no significant difference. Significant change (P0.05), T4 group and T2 compared with significant difference (P0.05).
(4) after 6 weeks of treadmill training and nutritional supplement, the changes of serum SOD and MDA in mice were found to vary in varying degrees.T1, T2, and the serum SOD content of the mice in group T4 was very significant (P0.01). There was a significant change in the T2 group (P0.05).T1, T2. There was no significant change in the serum content of the mice. There were significant differences (P0.05).
Conclusion: (1) long-term anaerobic exercise can increase the PK activity of skeletal muscle in mice, indicating that long-term anaerobic training can improve the ability of glycolysis by regulating the activity of metabolic enzymes.
(2) long-term aerobic training can effectively improve the SDH and MDH activity of skeletal muscle tissue in mice, indicating that long-term aerobic training can improve the capacity of aerobic energy supply of the body and increase the activity of the enzyme of the three carboxylic acid cycle.
(3) aerobic exercise has significantly increased aerobic metabolism, and the activity of anaerobic enzymes in anaerobic training has also increased correspondingly.
(4) supplementation of small molecular peptides from sea cucumbers can improve the activity of SDH, MDH and PK, which may be related to the conversion of the raw materials of SDH, MDH, PK to the improvement of anti fatigue ability.
(5) supplementation of small peptides from sea cucumber can improve the anti fatigue ability of mice.
【學(xué)位授予單位】：遼寧師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：G804.2

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