本文選題：放射性肺損傷 + 氫吡咯二硫代氨基甲酸酯　； 參考：《山東醫(yī)藥》2017年32期

【摘要】：目的探討氫吡咯二硫代氨基甲酸酯(PDTC)對(duì)大鼠放射性肺損傷的保護(hù)作用及其對(duì)肺組織核因子κB(NF-κB)、細(xì)胞間黏附分子1(ICAM-1)表達(dá)的影響。方法將60只Wistar大鼠隨機(jī)分為正常對(duì)照組、模型組和PDTC組,每組20只。模型組、PDTC組采用6 MV X射線照射雙肺制作放射性肺損傷模型。PDTC組照射前3天開始皮下注射PDTC 10 mg/kg,持續(xù)至照射后30天。正常對(duì)照組和模型組同期注射等體積生理鹽水。各組照射開始后8、24周處死后取肺組織,部分右肺組織行HE、Masson染色,觀察肺組織病理變化;取右肺中上葉,檢測(cè)羥脯氨酸表達(dá);取左肺組織,稱濕重,計(jì)算肺系數(shù);取右肺下葉,檢測(cè)肺組織ICAM-1、NF-κB mRNA表達(dá)。結(jié)果正常對(duì)照組右肺組織均未見明顯異常。照射開始后8周,模型組肺組織病變以滲出為主,并出現(xiàn)少許膠原纖維沉積;照射開始后24周,肺泡壁增厚,肺泡腔明顯變小,肺間質(zhì)內(nèi)各種細(xì)胞成分增多,可見明顯的局部灶性纖維化區(qū)域。PDTC組照射開始后8、24周肺組織病變較模型組同期明顯減輕,纖維化增厚主要集中在支氣管及血管外膜,肺泡區(qū)域幾乎未見明顯纖維化。模型組照射開始后8、24周肺組織羥脯氨酸表達(dá)、肺系數(shù)均高于正常對(duì)照組(P均0.05),PDTC組各時(shí)間點(diǎn)肺組織羥脯氨酸表達(dá)、肺系數(shù)較模型組同時(shí)間點(diǎn)明顯降低(P均0.05),但仍高于正常對(duì)照組同時(shí)間點(diǎn)(P均0.05)。模型組照射開始后8、24周肺組織ICAM-1、NF-κB mRNA相對(duì)表達(dá)量均高于正常對(duì)照組同時(shí)間點(diǎn)(P均0.05),PDTC組各時(shí)間點(diǎn)肺組織ICAM-1、NF-κB mRNA相對(duì)表達(dá)量均較模型組同時(shí)間點(diǎn)降低(P均0.05),且高于正常對(duì)照組(P均0.05)。結(jié)論 PDTC能減輕放射性肺損傷大鼠的肺部炎癥和纖維化程度,其機(jī)制可能與抑制NF-κB活化、調(diào)控ICAM-1表達(dá)有關(guān)。
[Abstract]:Objective to investigate the protective effect of hydropyrrolidine dithiocarbamate (PDTC) on radiation induced lung injury in rats and its effect on the expression of nuclear factor 魏 B (NF- 魏 B) NF- 魏 B, an intercellular adhesion molecule (ICAM-1). Methods 60 Wistar rats were randomly divided into normal control group, model group and PDTC group with 20 rats in each group. Radiation-induced lung injury model was induced by 6 MV X-ray irradiation in PDTC group. PDTC 10 mg / kg was injected subcutaneously 3 days before irradiation in PDTC group until 30 days after irradiation. The normal control group and the model group were injected with the same volume of saline at the same time. Eight weeks after irradiation, lung tissue was taken out, and some of the right lung tissues were stained with HE Masson to observe the pathological changes of the lung tissue; the middle and upper lobe of the right lung was taken to detect the expression of hydroxyproline; the left lung tissue was taken to weigh wet and calculate the lung coefficient; the lower lobe of the right lung was taken from the lower lobe of the right lung. The expression of NF- 魏 B mRNA in lung tissue was detected. Results there was no obvious abnormality in the right lung tissue in the normal control group. At 8 weeks after irradiation, the pathological changes of lung tissue in the model group were mainly exudate, and a little collagen fiber was deposited. 24 weeks after irradiation, the alveolar wall was thickened, the alveolar cavity was obviously smaller, and the various cellular components in the interstitial lung were increased. The pathological changes of lung tissue in PDTC group at 824 weeks after irradiation were obviously reduced compared with that in model group. The thickening of fibrosis was mainly concentrated in the bronchi and adventitia, and there was almost no obvious fibrosis in alveolar region. The lung hydroxyproline expression in the model group was significantly higher than that in the normal control group at 24 weeks after irradiation. Compared with the model group, the lung coefficient was significantly lower than that of the model group at the same time point (P < 0.05), but still higher than that of the normal control group at the same time point (P < 0.05). The relative expression of ICAM-1 NF- 魏 B mRNA in lung tissue in the model group was significantly higher than that in the normal control group at 24 weeks after irradiation (P < 0.05). The relative expression of ICAM-1NF- 魏 B mRNA in the lung tissue in the model group was lower than that in the model group at the same time point (P 0.05) and higher than that in the normal control group (P < 0.05). Conclusion PDTC can attenuate the degree of pulmonary inflammation and fibrosis in rats with radiation-induced lung injury. The mechanism may be related to the inhibition of NF- 魏 B activation and the regulation of ICAM-1 expression.
【作者單位】： 沈陽市第七人民醫(yī)院;鄭州大學(xué)第五附屬醫(yī)院;
【分類號(hào)】：R730.55

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