本文選題：腫節(jié)風 + 小型豬　； 參考：《廣西醫(yī)科大學》2012年碩士論文

【摘要】：目的：探討小型豬腮腺經(jīng)15Gy γ射線放射性損傷后,腫節(jié)風顆粒對外周血和腮腺ROS含量的影響,研究其可能的防護機制以及腮腺放射性損傷的作用機理。 方法：(1)采用實驗用巴馬小型豬作為實驗對象,建立腫節(jié)風干預下的腮腺放射性損傷實驗動物模型：將45頭小型豬隨機分入空白對照組(空白組)、單純照射組(單照組)以及腫節(jié)風+照射組(藥照組)3個大組,每組分成a、b、c三個平行組；在麻醉條件下單照組和藥照組均給予DT15Gy/l次γ射線照射雙側(cè)腮腺組織,空白組給予0Gy Y射線照射；藥照組照射前一周開始按照0.3g/Kg體重的藥量經(jīng)口腔給予腫節(jié)風配方顆粒溶液,空白組和單照組給予等量的生理鹽水,直至觀察結(jié)束。a、b、c三個平行組分別于照射后10d、40d、90d三個時間點取雙側(cè)腮腺,稱量后分裝。(2)在照射前1d和照射后第10d取腮腺標本前從前腔靜脈抽取小型豬的靜脈血進行血常規(guī)和血生化的檢測；(3)以豬活性氧簇(reactive oxygen species, ROS)試劑盒檢測照射后10d、40d、90d腮腺中ROS的變化。 結(jié)果：(1)成功建立起腫節(jié)風干預下的腮腺放射性損傷實驗動物模型。(2)照射后白細胞數(shù)和紅細胞明顯降低,但藥照組高于單照組,差異有統(tǒng)計學意義(P0.01)；單照組血小板明顯降低,而藥照組的血小板則高于放療前,差異有統(tǒng)計學意義(P0.01)；照射后乳酸脫氫酶的含量呈下降趨勢,與放療前差異有統(tǒng)計學意義(P0.01),單照組高于藥照組,差異有統(tǒng)計學意義(P0.01)；照射后單照組血淀粉酶明顯升高,而單照組高于藥照組,且差異有統(tǒng)計學意義(P0.01)。(3)藥照組ROS的含量在各個時點均低于單照組,差異均有統(tǒng)計學意義(P0.01),藥照a組和藥照b組、藥照c組的差異均無統(tǒng)計學意義(P0.01),藥照b組和藥照c組差異有統(tǒng)計學意義(P0.01)；在放射后10d時腫節(jié)風對ROS的清除作用較強,藥照組和與空白組的差異無統(tǒng)計學意義(P0.01)；放射后40d和90d天腫節(jié)風對ROS均有有一定的清除作用,藥照組與空白組的差異有統(tǒng)計學意義(P0.01)。 結(jié)論：(1)單次15Gy γ射線雙側(cè)單后野垂直照射腮腺組織可成功建立起腮腺放射性損傷的實驗動物模型；(2)腫節(jié)風對小型豬腮腺放射損傷后外周血的恢復有促進作用,可促進白細胞和紅細胞的恢復、減緩血淀粉酶的升高、提高血小板和乳酸脫氫酶的含量,腫節(jié)風可能通過促進外周血的恢復從而提高機體的免疫力,這可能是其對小型豬腮腺急性放射損傷具有防護作用的原因；(3)腫節(jié)風對小型豬腮腺放射損傷所致ROS有一定的清除作用,尤其對放射后10d腮腺ROS的清除作用明顯；清除放射所產(chǎn)生的ROS可能是腫節(jié)風防護腮腺放射損傷的主要機制。
[Abstract]:Objective: to investigate the effect of Zongjie Feng granule (ZJF) on ROS content in peripheral blood and parotid gland after 15Gy 緯 -ray radiation injury in miniature pig parotid gland, and to study the possible protective mechanism and the mechanism of radiation damage of parotid gland. Method 1) Bama miniature pig was used as the experimental object. To establish the experimental animal model of parotid gland radiation injury induced by TJV: 45 miniature pigs were randomly divided into three groups: blank control group (blank group), single irradiation group (single irradiation group) and radiation group (drug irradiation group). Each group was divided into three parallel groups, which were given DT15Gy/l 緯 -ray irradiation on both sides of parotid gland and 0Gy Y irradiation in blank group under anaesthesia condition. One week before irradiation, the drug dose of 0.3g/Kg was given to the oral cavity, and the blank group and the single irradiation group were given the same amount of normal saline, and the control group and the single irradiation group were given the same amount of normal saline. The bilateral parotid glands were taken from the three parallel groups at 10 days, 40 days and 90 days after irradiation until the end of observation. 1 day before irradiation and 10 days after irradiation, the venous blood of miniature pigs was extracted from the anterior vena cava for blood routine examination and biochemical examination. The changes of ROS in parotid gland were detected by reactive oxygen species, ROS) kit 10 days after irradiation. Results (1) the experimental animal model of radiation injury of parotid gland was successfully established. The number of white blood cells and red blood cells were significantly decreased after irradiation, but the difference was statistically significant (P 0.01), but the platelet count of single irradiation group was significantly lower than that of single irradiation group, and that of single irradiation group was significantly lower than that of single irradiation group (P < 0. 01). However, the platelet in the drug irradiation group was higher than that before radiotherapy, the difference was statistically significant (P 0.01), the content of lactate dehydrogenase decreased after irradiation, and the difference was statistically significant compared with that before radiotherapy, and the level of lactate dehydrogenase in the single irradiation group was higher than that in the drug irradiation group. The level of serum amylase in the single irradiation group was significantly higher than that in the single irradiation group, and the content of ROS in the single irradiation group was lower than that in the single irradiation group at all time points. There was no significant difference between group A and group C, but there was significant difference between group B and group C. there was a significant difference between group B and group C (P 0.01), and the removal of ROS was stronger than that of group C at 10 days after radiation, but there was no significant difference between group C and group B (P 0.01), but there was no significant difference between group C and group A (P 0.01). There was no significant difference between the radiopharmaceutical group and the blank group (P 0.01), but 40 d and 90 d after irradiation, the ROS was cleared in both groups, and the difference between the group and the blank group was statistically significant (P 0.01). Conclusion single 15Gy 緯 -ray irradiation on parotid gland can successfully establish the experimental animal model of parotid gland radiation injury. It can promote the recovery of peripheral blood after parotid gland radiation injury in miniature pigs. It can promote the recovery of white blood cells and red blood cells, slow down the rise of serum amylase, increase the contents of platelet and lactate dehydrogenase, and increase the immunity of the body by promoting the recovery of peripheral blood. This may be the reason why it has protective effect on acute radiation injury of parotid gland in miniature pigs. It has a certain scavenging effect on ROS induced by radiation injury of parotid gland in miniature pig, especially on ROS clearance of parotid gland 10 days after radiation. ROS caused by scavenging radiation may be the main mechanism of protecting parotid gland from radiation injury.
【學位授予單位】：廣西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R818

【參考文獻】

相關期刊論文 前10條

1 利國威,冼超貴,雷穗妮,陳昆田;黃岑中酚性甙類對小鼠放射損傷防護作用的研究[J];癌癥;1999年04期

2 冼超貴,王漢渝,利國威,劉甘泉,古模發(fā),李曉君;黃芩中酚性甙類對小鼠頜下腺放射損傷防護的研究[J];癌癥;2000年08期

3 單兆臣,王松靈,李鈞,祁森榮,張春梅;小型豬腮腺放射損傷唾液流率和血常規(guī)及生化動態(tài)觀察[J];北京口腔醫(yī)學;2003年04期

4 陳月,王寶貴,張桂英,顏煒群;刺五加皂苷的抗輻射損傷作用[J];吉林大學學報(醫(yī)學版);2005年03期

5 張海東;王仁生;馬姍姍;梁菲菲;肖帥;黎國尖;;腫節(jié)風顆粒對小型豬腮腺放射損傷所致活性氧簇的清除作用[J];南方醫(yī)科大學學報;2011年01期

6 湯春園;秦儉;王仁生;;腫節(jié)風水提物抗放射誘導氧化損傷的臨床觀察[J];國際檢驗醫(yī)學雜志;2008年10期

7 韋波;王仁生;秦儉;張勇;滕家安;;腫節(jié)風水提物減輕放射性口干的臨床療效觀察[J];廣西醫(yī)科大學學報;2009年02期

8 王仁生;黃國軍;韋波;蔣偉哲;吳芳;劉文其;;腫節(jié)風浸膏溶液對鼻咽癌細胞系CNE-2的放射增敏作用[J];廣西醫(yī)科大學學報;2009年02期

9 翁侯年;;中醫(yī)藥治療口干癥126例[J];湖北中醫(yī)雜志;1983年04期

10 李培峰，，方允中;抗壞血酸－Fe（Ⅲ）對銅鋅超氧化物歧化酶理化性質(zhì)的影響[J];軍事醫(yī)學科學院院刊;1994年04期

相關會議論文 前1條

1 王文凱;雷丹;;中藥腫節(jié)風的研究概況[A];中華中醫(yī)藥學會中藥炮制分會2008年學術(shù)研討會論文集[C];2008年

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