本文選題：~(125)I + 分子探針�。� 參考：《川北醫(yī)學院》2012年碩士論文

【摘要】：目的：探索125I-Mouse-Anti-Muc4標記方法，選擇最佳標記條件，并進行質量鑒定，為進一步了解分子探針在體內的生物學行為以及胰腺癌的放射免疫顯像和治療奠定基礎。 方法：本實驗分別采用Iodogen、NBS作為氧化劑制備分子探針—125I-Mouse-Anti-Muc4，其它條件固定時，依次改變反應溫度、時間和碘與抗體、Iodogen/ NBS與抗體用量，用紙層析法測定標記產物的標記率，探索最佳的標記反應條件；然后將反應物經PD-10脫鹽柱淋洗，收集蛋白峰管，測定放射性活度，并測定純化后的放化純；并測定標記物在不同儲存溶劑中的體外穩(wěn)定性。 結果：Iodogen、NBS法標記Mouse-Anti-Muc4的最高標記率分別為（81.3±0.97）%、（50.7±0.79）%，Iodogen為比較理想的氧化劑；不同的反應溫度、時間和碘與抗體、Iodogen/ NBS與抗體用量可影響標記效果，當在室溫（25~0C）下反應15min，，碘/抗體比值為1.5，Iodogen/抗體比值為10時標記率最高；~(125)I- Mouse-anti-Muc4的放化純達（95.6±1.26）%；125I-Mouse-anti-Muc4在生理鹽水及1%胎牛血清中放置48小時內放化純維持在90%左右。 結論：首次使用~(125)I標記Mouse-anti-Muc4成功獲得了分子探針—125I-Mouse-anti-Muc4；Iodogen法標記Mouse-anti-Muc4是比較理想的方法，能夠得到比較理想的標記率及純化率；不同的反應溫度、時間和碘與抗體、Iodogen/ NBS與抗體用量可影響其標記效果；~(125)I-Mouse-anti-Muc4體外穩(wěn)定性好；分子探針—~(125)I-Mouse-anti-Muc4的免疫活性、生物學行為及其胰腺癌的放射免疫顯像和治療等方面值得進一步研究。
[Abstract]:Objective: to explore the method of 125I-Mouse-Anti-Muc4 labeling, to select the best labeling conditions, and to identify the quality of the probe, so as to lay a foundation for further understanding the biological behavior of the molecular probe in vivo and the radioimmunoimaging and treatment of pancreatic cancer. Methods: the molecular probe -125I-Mouse-Anti-Muc4 was prepared by using Iodogen-NBS as oxidant. When the other conditions were fixed, the reaction temperature, reaction time and the amount of iodide / NBS and antibody were changed in turn. The labeling rate of labeled products was determined by paper chromatography. The best labeling reaction conditions were explored, then the reactants were eluted by PD-10 desalination column, protein peak tubes were collected, radioactivity was determined, radiochemical purity was determined, and the stability of the reactants in different storage solvents in vitro was determined. Results the highest labeling rates of Mouse-Anti-Muc4 labeled by the 7% Iodogen method were 81.3 鹵0.97 and 50.7 鹵0.79, respectively, and Iodogen was the ideal oxidant. Different reaction temperature, reaction time, iodine / NBS and antibody dosage could affect the labeling effect. The radiochemical purity of 125I-Mouse-anti-Muc4 was 95.6 鹵1.26C125I-Mouse-anti-Muc4 in normal saline and 1% fetal bovine serum for about 90% within 48 hours after the reaction at room temperature for 15 min, and the ratio of iodine / antibody was 1.5% Iodogen-antibody / antibody ratio was 10:00. The radiochemical purity of 125I-Mouse-anti-Muc4 in normal saline and 1% fetal bovine serum was about 90%. Conclusion: the molecular probe -125I-Mouse-anti-Muc4- Iodogen method was successfully obtained for the first time in the labeling of Mouse-anti-Muc4, and the labeling rate and purification rate were obtained, and the reaction temperature was different, and the molecular probe -125I-Mouse-anti-Muc4- Iodogen method was used to label Mouse-anti-Muc4 successfully for the first time. Time and dosage of Iodogen / NBS and antibody could affect the stability of 125I-Mouse-anti-Muc4 in vitro, and the immunological activity, biological behavior and radioimmunoimaging and treatment of pancreatic cancer could be further studied by molecular probe -125I-Mouse-anti-Muc4.
【學位授予單位】：川北醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R817.4;R735.9

【參考文獻】

相關期刊論文 前10條

1 馬霄虹;周純武;;磁共振功能成像在胰腺癌診斷中的應用及新進展[J];癌癥進展;2009年06期

2 張維;趙建農;;聯(lián)合運用肝臟三維容積超快速多期動態(tài)增強技術及擴散加權成像在胰腺癌診斷中的價值[J];第三軍醫(yī)大學學報;2008年20期

3 張興龍;吳育連;;5種影像學檢查方法對胰腺癌診斷價值的比較[J];肝膽胰外科雜志;2009年02期

4 李亮;袁家長;靳二虎;;磁共振膽胰管成像顯示分支胰管擴張的意義[J];國際醫(yī)學放射學雜志;2009年02期

5 吳玉純,許吟秋,孫貴海,鄭一丘;螺旋CT動態(tài)掃描對胰腺癌的早期診斷[J];哈爾濱醫(yī)藥;2004年02期

6 汪靜,王喜青,王連剛,李富軍,鄧敬蘭;~(125)I-RC-160的標記方法研究及其正常小鼠體內的分布特征[J];核技術;2002年11期

7 翟士軍;章斌;袁航;蘇成海;;Iodogen法~(125)I標記單克隆抗體10D9[J];核技術;2009年05期

8 李曉輝;熊炯p

本文編號：1849363