本文選題：交替低氧訓(xùn)練　切入點(diǎn)：骨骼肌　出處：《中國運(yùn)動醫(yī)學(xué)雜志》2017年01期

【摘要】：目的:探討不同海拔高度交替低氧訓(xùn)練對大鼠骨骼肌線粒體呼吸功能的影響。方法:50只雄性Wistar大鼠隨機(jī)分為5組:常氧對照組(C組)、常氧訓(xùn)練組(NT組)、2500 m低氧訓(xùn)練組(2500 m HT組)、3500 m低氧訓(xùn)練組(3500 m HT組)、2500 m~3500 m交替低氧訓(xùn)練組(2500 m~3500 m HT組),每組10只。除C組大鼠不進(jìn)行運(yùn)動訓(xùn)練外,其余各組大鼠分別在常氧,模擬海拔2500m、3500 m及2500m~3500 m交替環(huán)境下進(jìn)行遞增負(fù)荷跑臺訓(xùn)練和居住,每周訓(xùn)練6天,共4周。訓(xùn)練結(jié)束后處死大鼠,即刻取全血和骨骼肌樣本,梯度離心法提取線粒體,測定血液紅細(xì)胞數(shù)目(RBC)、血紅蛋白(Hb)含量和骨骼肌線粒體呼吸鏈酶復(fù)合體(CⅠ~Ⅳ)活性。結(jié)果:(1)RBC和Hb:與C組比較,各訓(xùn)練組都有顯著提高(P0.01,P0.05)。與NT組比較,低氧訓(xùn)練組都有顯著提高(P0.01)。與2500 m HT組和3500 m HT組比較,2500 m~3500 m HT組均顯著提高(P0.01)。(2)CⅠ~Ⅳ活性:與C組相比,NT組CⅢ活性顯著提高(P0.05),2500 m HT組CI活性顯著提高(P0.05),3500 m HT組CⅠ和CⅢ活性顯著提高(P0.01,P0.05),2500 m~3500 m HT組CⅠ~Ⅳ活性均顯著提高(P0.01,P0.05)。與NT組相比,3500 m HT組CⅠ活性顯著提高(P0.01),2500 m~3500 m HT組CⅠ、CⅡ活性顯著提高(P0.01,P0.05)。與2500 m HT組相比,2500 m~3500 m HT組CⅡ活性顯著提高(P0.05)。結(jié)論:低氧訓(xùn)練在提高骨骼肌線粒體呼吸鏈功能方面較常氧訓(xùn)練更具優(yōu)勢,且交替低氧訓(xùn)練效果最佳。
[Abstract]:Aim: to investigate the effects of alternate hypoxia training at different altitudes on mitochondrial respiratory function in rat skeletal muscle.Methods Fifty male Wistar rats were randomly divided into 5 groups: control group (n = 10), control group (n = 10), NT group (n = 10), hypoxia training group (n = 2 500 m), hypoxia training group (n = 3 500 m HT), alternate hypoxic training group (n = 2 500 m) and control group (n = 10).Except for group C rats who did not exercise training, the rats in other groups were trained and lived under the alternating environment of 2500mg, 3500m and 2500m~3500 m, respectively, 6 days a week for 4 weeks.After training, the rats were killed and the whole blood and skeletal muscle samples were collected immediately. Mitochondria were extracted by gradient centrifugation. The contents of RBCU, HB) and the activity of mitochondrial respiratory chain enzyme complex (C 鈪，

本文編號：1727164