本文選題：運(yùn)動(dòng)性低血色素　切入點(diǎn)：血紅素轉(zhuǎn)運(yùn)蛋白1　出處：《河北師范大學(xué)》2012年碩士論文

【摘要】：鐵在運(yùn)動(dòng)中發(fā)揮重要作用。運(yùn)動(dòng)性低血色素的發(fā)生往往嚴(yán)重影響運(yùn)動(dòng)員的運(yùn)動(dòng)能力。機(jī)體主要通過調(diào)控十二指腸鐵吸收來維持機(jī)體鐵穩(wěn)態(tài)。血紅素轉(zhuǎn)運(yùn)蛋白1(heme carrier protein1，HCP1)、二價(jià)金屬離子轉(zhuǎn)運(yùn)體(divalent metal transporter1，DMT1)及膜鐵轉(zhuǎn)運(yùn)蛋白1（Ferroportin1，F(xiàn)PN1）是近年來發(fā)現(xiàn)的腸鐵吸收相關(guān)蛋白，參與十二指腸鐵的吸收和釋放并在腸鐵吸收轉(zhuǎn)運(yùn)中起關(guān)鍵作用。HCP1和DMT1可分別將食物中的血紅素鐵和非血紅素鐵轉(zhuǎn)運(yùn)到十二指腸上皮吸收細(xì)胞內(nèi)，，而FPN1將十二指腸上皮細(xì)胞內(nèi)的鐵轉(zhuǎn)運(yùn)到胞外，通過血液循環(huán)將鐵運(yùn)輸至各個(gè)器官，供機(jī)體進(jìn)行各種生理活動(dòng)。然而運(yùn)動(dòng)中腸鐵吸收的具體變化過程還不甚清楚，尤其是運(yùn)動(dòng)性低血色素形成中機(jī)體通過何途徑如何調(diào)節(jié)小腸鐵轉(zhuǎn)運(yùn)蛋白的表達(dá)，還需進(jìn)一步研究。因此，本文研究了長(zhǎng)時(shí)間遞增負(fù)荷跑臺(tái)運(yùn)動(dòng)導(dǎo)致的運(yùn)動(dòng)性低血色素形成中，大鼠腸鐵吸收蛋白HCP1、DMT1和FPN1表達(dá)的動(dòng)態(tài)變化，為運(yùn)動(dòng)性低血色素的預(yù)防和治療提供實(shí)驗(yàn)和理論依據(jù)。 研究?jī)?nèi)容與方法 36只雄性Wistar大鼠，隨機(jī)分為對(duì)照組（control group，CG）和運(yùn)動(dòng)組（exercisegroup，EG）。對(duì)照組和運(yùn)動(dòng)組又分別分為3周、4周、5周組，運(yùn)動(dòng)組大鼠進(jìn)行6d/w、坡度為0、速度30m/min的遞增負(fù)荷跑臺(tái)訓(xùn)練。前2周每天訓(xùn)練1次，時(shí)間從1min開始，每次遞增2min，從第3周開始，每天訓(xùn)練2次。分別于運(yùn)動(dòng)第3、4、5周末取材，與其相對(duì)應(yīng)的對(duì)照組也同時(shí)取材。采用血細(xì)胞自動(dòng)分析儀測(cè)定血紅蛋白（Hb）含量；分光光度計(jì)測(cè)血清鐵含量；Western Blot檢測(cè)十二指腸上皮細(xì)胞HCP1、DMT1及FPN1表達(dá)。 研究結(jié)果 1、運(yùn)動(dòng)性低血色素形成中大鼠血紅蛋白（Hb）的變化：經(jīng)過長(zhǎng)時(shí)間大強(qiáng)度運(yùn)動(dòng)后大鼠Hb含量逐漸降低。運(yùn)動(dòng)組大鼠3周Hb與其對(duì)照組相比有下降趨勢(shì)，4周和5周顯著低于其對(duì)照組（P 0.05，P 0.01）。 2、運(yùn)動(dòng)性低血色素形成中大鼠血清鐵含量的變化：經(jīng)過遞增負(fù)荷運(yùn)動(dòng)后大鼠血清鐵含量先增加后降低。運(yùn)動(dòng)組大鼠3周血清鐵含量與其對(duì)照組相比顯著增加（P 0.05）；4周運(yùn)動(dòng)組大鼠血清鐵含量與其對(duì)照組相比稍有減少但變化不明顯；5周運(yùn)動(dòng)組大鼠血清鐵含量與其對(duì)照組相比極顯著減少（P 0.01）。 3、運(yùn)動(dòng)性低血色素形成中大鼠十二指腸上皮吸收細(xì)胞鐵吸收蛋白的變化：運(yùn)動(dòng)組大鼠3周十二指腸上皮細(xì)胞HCP1、DMT1及FPN1表達(dá)均顯著高于其對(duì)照組（P0.01），4周與其對(duì)照組相比均無顯著差異（P0.05），5周均顯著低于對(duì)照組（P0.01）。 研究結(jié)論 長(zhǎng)時(shí)間大強(qiáng)度運(yùn)動(dòng)開始階段，機(jī)體通過增加十二指腸鐵吸收維持運(yùn)動(dòng)機(jī)體對(duì)鐵的需求，但隨運(yùn)動(dòng)時(shí)間延長(zhǎng)，機(jī)體腸鐵吸收和釋放能力降低，導(dǎo)致血清鐵含量減少，這是引發(fā)運(yùn)動(dòng)性低血色素的重要原因之一。
[Abstract]:Iron plays an important role in sports.The occurrence of sports hypohemoglobin often seriously affects the athletic ability of athletes.The body maintains iron homeostasis by regulating the absorption of iron in the duodenum.The heme transporter 1(heme carrier protein 1 (HCP1), divalent metal transporter1 (DMT1) and membrane iron transporter (1) Ferroportin 1 (FPN1) are identified as iron absorption related proteins in the intestine.Participate in the absorption and release of iron from duodenum and play a key role in the absorption and transport of iron in intestine. HCP1 and DMT1 can transport heme iron and non heme iron from food into the absorption cells of duodenal epithelium, respectively.FPN1 transports iron from the duodenal epithelial cells to the extracellular, and transports iron to various organs through blood circulation for various physiological activities.However, the specific process of iron absorption in the intestine during exercise is not clear, especially the regulation of the expression of small intestinal iron transporter by the body in the process of exercise hypohemoglobin formation, which needs further study.Therefore, the dynamic changes of the expression of iron absorption protein HCP1DMT1 and FPN1 during the formation of exercise hypohemoglobin induced by prolonged incremental treadmill exercise in rats were studied in order to provide experimental and theoretical basis for the prevention and treatment of exercise hypohemoglobin.Research contents and methodsThirty-six male Wistar rats were randomly divided into two groups: control group (CGG) and exercise group (exercise group).The control group and the exercise group were divided into 3 weeks and 4 weeks and 5 weeks groups respectively. The rats in the exercise group were trained with incremental load running for 6 d / w with a slope of 0 and a speed of 30m/min.The first 2 weeks were trained once a day, the time began from 1min, each time increased by 2 mins, from the 3rd week, 2 times a day.The materials were collected at the end of the 5th week of exercise, and the control group was also taken at the same time.The content of hemoglobin (HB) and the expression of HCP1DMT1 and FPN1 in duodenal epithelial cells were determined by automatic blood cell analyzer and serum iron content by spectrophotometer and Western Blot respectively.Research results1. The changes of hemoglobin (HB) in rats during exercise induced hypohemoglobin formation: after a long time and intensive exercise, the HB content of rats decreased gradually.Compared with the control group, HB in the exercise group decreased significantly at 4 and 5 weeks, which was significantly lower than that in the control group at 4 and 5 weeks.2. Changes of serum iron content in exercise induced hypohemoglobin formation: after increasing load exercise, the serum iron content of rats increased first and then decreased.The serum iron content in the exercise group was significantly higher than that in the control group at 3 weeks. The serum iron content in the exercise group was slightly lower than that in the control group, but there was no significant change in the serum iron content between the exercise group and the control group.Compared with the control group, there was a significant decrease of P 0.01.3, changes of iron absorption protein in duodenal epithelial resorption cells of rats during exercise hypohemoglobin formation: the expression of HCP1DMT1 and FPN1 in duodenal epithelial cells in exercise group was significantly higher than that in control group at 4 weeks after 3 weeks of exercise compared with that in control group.There was no significant difference in P0. 05 and P0. 01 in the control group at 5 weeks.Research conclusionAt the beginning of a long period of high intensity exercise, the body maintained the iron demand by increasing the absorption of iron in duodenum, but with the extension of exercise time, the absorption and release ability of iron in intestine decreased, which resulted in the decrease of iron content in serum.This is one of the important causes of exercise-induced hypohemoglobin.
【學(xué)位授予單位】：河北師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R87

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