本文選題：唾液腺　切入點(diǎn)：放射性損傷　出處：《山東醫(yī)藥》2017年41期

【摘要】：目的探討單次大劑量與多次小劑量放射線構(gòu)建小鼠放射性唾液腺損傷模型的應(yīng)用效果。方法選取C3H小鼠30只,隨機(jī)分為空白對(duì)照組(空白組)、單次大劑量照射組(單放組)、多次小劑量照射組(多放組)各10只。空白組以0 Gy放射線照射,單放組以15 Gy放射線單次照射,多放組以每天5 Gy照射,連續(xù)5 d。分別于照射前及照射后1、5、10周測(cè)定各組唾液分泌率。照射后10周采集三組小鼠下頜下腺,分別測(cè)定腺泡細(xì)胞相對(duì)面積、細(xì)胞增殖率及毛細(xì)血管密度。結(jié)果與空白組比較,單放組、多放組唾液分泌率均下降,腺泡細(xì)胞相對(duì)面積減小,下頜下腺細(xì)胞增殖率降低,下頜下腺毛細(xì)血管密度減低,差異均有統(tǒng)計(jì)學(xué)意義(P均0.05)。單放組與多放組以上觀察指標(biāo)比較差異無統(tǒng)計(jì)學(xué)意義(P均0.05)。結(jié)論單次大劑量與多次小劑量放射線構(gòu)建小鼠放射性唾液腺損傷模型的效果相當(dāng)。
[Abstract]:Objective to investigate the effect of single dose and multiple low dose radiation on the model of radioactive salivary gland injury in mice.Methods Thirty C3H mice were randomly divided into blank control group (blank group), single large dose irradiation group (single dose group) and multiple low dose irradiation group (multiple dose group).The blank group was irradiated with 0 Gy radiation, the single radiation group with 15 Gy single dose radiation, and the multiple radiation group with 5 Gy radiation per day for 5 days.Saliva secretion rate was measured before irradiation and 10 weeks after irradiation.The submandibular glands of the three groups were collected 10 weeks after irradiation, and the relative area of acinar cells, cell proliferation rate and capillary density were measured.Results compared with the blank group, the salivary secretion rate, the relative acinar cell area, the proliferation rate and the capillary density of the submandibular gland in the single and multiple radiation groups were all decreased (P < 0.05).There was no significant difference in the above indexes between the single group and the multiple group (P < 0.05).Conclusion the effects of single large dose irradiation and multiple low dose radiation on mice with radioactive salivary gland injury are similar.
【作者單位】： 勝利油田中心醫(yī)院;
【分類號(hào)】：R-332;R730.55
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本文編號(hào)：1700496